07/20/2020

Publications

Basic Science and Technology

1. Gruhot, T.R., Rempel, L.A., White, B.R., Mote, B.E. 2020. The effect of varicocele on semen quality in boars exposed to heat stress. Translational Animal Science. 4(1):1-6. https://doi.org/10.1093/tas/txaa003.


2. Kerns K, Sharif M, Zigo M, Miller D, Sutovsky S. 2020. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. Int J Mol Sci 21:2121.


3. Rubessa, M., Feugang, J.M., Kandel, M.E., Schreiber, S., Hessee, J., Salerno, F., Meyers, S., Chu, I., Popescu, G., Wheeler, M.B. 2020. High-throughput sperm assay using label-free microscopy: morphometric comparison between different sperm structures of boar and stallion spermatozoa. Animal Reproduction Science. 106509. https://doi.org/10.1016/j.anireprosci.


4. Sharif M, Kerns K, Sutovsky P, Bovin N, Miller. DJ Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner. Submitted.


5. Walsh, S.C., Miles, J.R., Yao, L., Broeckling, C.D., Rempel, L.A., Wright-Johnson, E.C., Pannier, A.K. 2020. Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation. Journal of Molecular Reproduction and Development. 87:174-190. https://doi.org/10.1002/mrd.23306.


6. Boettcher AN, Kiupel M, Adur MK, Cocco E, Santin AD, Bellone S, Charley SE, Blanco-Fernandez B, Risinger JI, Ross JW, Tuggle CK, Shapiro EM. Human Ovarian Cancer Tumor Formation in Severe Combined Immunodeficient (SCID) Pigs. Front Oncol. 2019 Jan 22;9:9. doi: 10.3389/fonc.2019.00009. eCollection 2019. PMID: 30723704.


7. Schultz, R., Serao, N.L., Ross, J.W. 2019. Genetic improvement of livestock, from conventional breeding to biotechnological approaches. In: Animal Agriculture:Sustainability, Challenges and Innovations. Eds. Bazer, F.W., Wu, G. In Press (Elsevier).


8. Mayorga E.J., Renaudeau, D., Ramirez, B.C., Ross, J.W., Baumgard, L.H. 2019. Heat stress adaptation in pigs. Animal Frontiers 9:54-61.

Boar Performance

1. Feugang JM, Rhoads CE, Mustapha PO, Tardif S, Parrish JJ, Willard ST, Ryan PL. 2019. Treatment of boar sperm with nanoparticles for improved fertility. Theriogenology 137:75-81.


2. Hufana-Duran D, Duran PG, Venturina E, Peralta MD, Parrish JJ. 2019. Predicting bull fertility by sperm nuclear shape in water buffalo (bubalus bubalis). Thai J. Vet Med 49:75-81.


5. Lugar, D.W., Harlow, K.E., J. Hundley, M. Goncalves, J. Bergstrom, and K.R. Stewart. 2019. Effects of increased levels of supplemental vitamins during the summer in a commercial AI boar stud. Animal 1-13. doi:10.1017/S1751731119001150

Genetics and Genomics

1. Wijesena, H.R., Rohrer, G.A., Nonneman, D.J., Keel, B.N., Petersen, J.L., Kachman, S.D., Ciobanu, D.C. 2019. Evaluation of genotype quality parameters for SowPro90, a novel genotyping platform for swine. Journal of Animal Science. 97(8):3262-3273. https://doi.org/10.1093/jas/skz185.


2. Lents, C.A., Lindo, A.N., Hileman, S.M., Nonneman, D.J. 2020. Physiological and genomic insight into neuroendocrine regulation of puberty in gilts. Domest Anim Endocrinol. 11:106446. doi: 10.1016/j.domaniend.2020.106446.

Sow and Gilt Performance

1. Harlow, K., Ferreira, C.R., Sobreira, T.J.P., Casey, T. and K.R. Stewart.   Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet. PLoS ONE 14(9): e0215186. https://doi.org/10.1371/journal.pone.0215186


2. Harlow, K., Suarez-Trujillo, A., Hedrick, V., Sobreira, T.J.P., Aryal, U.K., Stewart, K.R. and T. Casey. Temporal analysis of vaginal proteome reveals developmental changes in lower reproductive tract of gilts across the first two weeks of postnatal.  Sci Rep. 2019 Sep 13;9(1):13241. doi: 10.1038/s41598-019-49597-w. 


3. Leonard, S.M., Xin, H., Brown-Brandl, T.M., Ramirez, B.C., Dutta, S., Rohrer, G.A. 2020. Effects of farrowing stall layout and number of heat lamps on sow and piglet production performance. Animals. 10:348. https://doi.org/doi:10.3390/ani10020348.


4. Seibert, J.T., Adur, M.K., Schultz, R.B., Thomas, P.Q., Kiefer, Z.K., Keating, A.F., Baumgard, L.H., Ross, J.W. 2019. Differentiating between the effects of heat stress and lipopolysaccharide on the porcine ovarian heat shock protein response. J Anim Sci. 97:4965-4973. doi: 10.1093/jas/skz343. PMID: 31782954.


5. Bidne KL, Romoser MR, Ross JW, Baumgard LH, Keating AF. 2019. Heat stress during the luteal phase decreases luteal size but does not affect circulating progesterone in gilts1. J Anim Sci. 97:4314-4322. doi: 1093/jas/skz251.


6. Hines EA, Romoser MR, Kiefer ZE, Keating AF, Baumgard LH, Niemi J, Gabler NK, Patience JF, Haberl B, Williams NH, Kerr BJ, Touchette KJ, Ross JW. 2019. The impact of dietary supplementation of arginine during gestation in a commercial swine herd: I. Gilt reproductive performance. J Anim Sci. 97:3617-3625. doi: 10.1093/jas/skz233.

Teaching and Extension

1. Boyd, R., Zier-Rush, C., Moeser, A., Culbertson, M., Stewart, K., Rosero, D., & Patience, J. 2019. Review: Innovation through research in the North American pork industry. Animal, 13(12), 2951-2966. doi:10.1017/S1751731119001915

 

Abstracts/Posters/Professional Presentations

1. Adur, M.K., Romoser, M.R., Bidne, K.L., Schultz, R.B., Keating, A.F., Baumgard, L.H., Ross, J.W. 2019 Proteomic assessment of the intra-uterine secretome during the peri- implantation phase to understand effects of increased ambient temperature on the porcine heat stress 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.


2. Corredor, F.A., Sanglard, L.P., Ross, J.W., Keating, A.F., Serao, N.L. 2019. Phenotypic and genomic relationships between vulva score and reproductive performance in first- parity sows. American Society of Animal Science Midwest Section Meeting. Omaha, March 11-13.


3. Desaulniers, A., Cederberg, R., Knox, R., Lents, C.A., White, B. 2019. Use of genetic engineering to elucidate reproductive physiology of the boar. In: Missouri Physiological Society 2019 Annual Meeting Abstracts, 26Oct2019, Columbia, MO. pp. 6.


4. Doungkamchan K, Parrish JJ, Miller DJ. 2020. Proteomic identification of molecular pathways and biomarkers related to porcine seasonal heat-induced infertility in males. 53rd Annual Meeting of the Society for the Study of Reproduction.


5. Ebrecht, M.A., Ross, C.E., Cederberg, R.A., Lovercamp, K.W., Desaulniers, A.T., White, B.R. 2019. Effects of GnRH-II on spermatogenic function of swine. Proceedings of the 16th Annual Gilbert S. Greenwald Symposium on Reproduction and Perinatal Research. p. 44.


6. Kerns K, Sharif M, Zigo M, Miller DJ, Sutovsky P. 2019. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. 52^th Annual Meeting of the Society for the Study of Reproduction.


7. Kiefer, Z.E., Chipman, A.L., Studer, J., Johnson, C., Rademacher, C.J., Linhares, D.C.L., Ross, J.W. 2019. Update on Prolapses and Pig Survivability Initiative. James D. McKean Swine Disease Conference. Ames, Iowa. November 7-8.


8. Kiefer, Z.E., Chipman, A.L., Studer, J.M., Koester, L.R., Showman, L., Keating, A.F., Schmitz-Esser, , Ross, J.W. 2019. Identification of putative factors associated with pelvic organ prolapse in sows during late gestation. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.


9. Knox RV, Belstra BA, Willenburg KL, D Gómez-López DH, and Stewart KR.   Effects of number of sperm and site of uterine semen deposition on conception rate and number of embryos in weaned sows receiving a single fixed time insemination. IXth International Conference on Boar Semen Preservation. Sydney, Australia


10. Mills, K., U.K. Aryal, T. Sobreira, A.M. Minton, T. Casey, K.R. Stewart. 2020. Shotgun proteomics reveal seminal plasma proteomes are reflective of boar reproductive performance. 2020 Midwest ASAS.


11. Park, S.B., Gruhot, T.R., Liao, S.F., Rude, B.J., Mote, B.E., Feugang, J.M. 2019. Favorable seminal plasma environment to sperm fertility after L-arginine addition to boar diet. 42^nd International Conference of Boar Semen Preservation (ICBSP), Hunter Valley, NSW, Australia.


12. Rohrer, G.A., Cross, A.J., Keel, B.N., Brown-Brandl, T.M., Cassady, J.P. 2019. Genetic factors associated with changes in feeding behavior due to elevated temperature. In: Proceedings of the Plant and Animal Genome Conference, San Diego, CA, Jan. 12-15, 2019. pg. 8, W034. Available: https://www.intlpag.org/2019/images/pdf/2019/PAGXXVII-abstracts-workshops.pdf


13. Ross, C.E., Choat, F.H., Plager, K.N., Desaulniers, A.T., Cederberg, R.A., Mills, G.A., White, B.R. 2019. Effect of GnRH-II and its receptor on boar semen quality. Reproduction, Fertility and Development. 32(2):201. https://doi.org/10.1071/RDv32n2Ab53.


14. Ross, J.W., Hale, B.J., Seibert, J.T., Adur, M.K., Keating, A.F., Baumgard, L.H. 2019. Physiological mechanisms through which heat stress compromises reproduction in pigs. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.


15. Schultz, R.B., Bidne, K.L., Romoser, M.R., Adur, M.K., Baumgard, L.H., Keating A.F., Ross, J.W. 2019. Effect of heat stress on the corpus luteum proteome during early pregnancy establishment in pigs. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.


16. Sharp, K., Mills, K., Garcia, R., K.R. Stewart.   Impacts of various farrowing induction protocols in attended farrowings.  Midwest ASAS, Omaha, NE.


17. Shirley, L.K., L.K. Shirley, M. Burgett,A.P. Schinckel, T. Field, R. Garcia, J.S. Johnson, K.R. Stewart, R.M. Stwalley. Evaluations of cooling pads on late lactation sows.  Midwest ASAS, Omaha, NE.


18. Stewart, K.R., T. Casey, K. Mills, U.K. Aryal and A.M. Minton. Shotgun proteome anaylsis of seminal plasma proteins differentiate boars by reproductive performance.  ICBSP, Hunter Valley, Australia.


19. Studer, J.M., Kiefer, Z.E., Keating, A.F., Baumgard, L.H., Olsen, K.M., Rambo, Z., Wilson, M.E., Rapp, C., Ross, J.W. 2019. Investigating the impact of manganese supplementation on corpus luteum function. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.


20. Supakorn, C., Lents, C.A., Martinez, X., Vallet, J.L., Boyd, R.D., Rohrer, G.A., Dedecker, A.E., Stalder, K.J. 2019. Associations between day one piglet serum immunocrit ratio and subsequent growth during gilt development [abstract]. Journal of Animal Science. 97 (Supplement 2):146. Abstract 352. https://doi.org/10.1093/jas/skz122.258.


21. Walsh, S., Miles, J.R., Wright-Johnson, E.C., Keel, B.N., Rempel, L.A., Pannier, A.K. 2019. Transcriptomic profiles of uniform populations of in vivo-produced spherical, ovoid, or tubular porcine embryos during the initiation of elongation. Reproduction, Fertility and Development. 32(2):152. https://doi.org/10.1071/RDv32n2Ab53.

07/12/2021

Basic Science and Technology

1. Wijesena, H.R., Kachman, S.D., Lents, C.A., Riethoven, J.J., Trenhaile-Grannemann, M.D., Safranski, T.J., Spangler, M.L., Ciobanu, D.C. 2020. Fine mapping genetic variants associated with age at puberty and sow fertility using Sowpro90 genotyping array. Journal of Animal Science. 98(10):1-12. https://doi.org/10.1093/jas/skaa293.


2. Azain, M., Cline, P.M., Tsai, T.C., Lents, C.A., Stelzleni, A.M., Dove, C.R. 2020. Interaction of dietary carbohydrate and fat on glucose metabolism in growing pigs. Research Square. pp. 22. https://doi.org/10.21203/rs.3.rs-48869/v1.


3. Nastasijevic, I., Schmidt, J.W., Boskovic, M., Glisic, M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M., Bosilevac, J.M. 2020. Seasonal prevalence of Shiga toxin-producing Escherichia coli on pork carcasses for three steps of the harvest process at two commercial processing plants in the United States. Applied and Environmental Microbiology. 87(1):e01711-20. https://doi.org/10.1128/AEM.01711-20.


4. Chaudhari, J., Liew, C., Workman, A.M., Riethoven, J.M., Steffen, D., Sillman, S., Vu, H.L.X. 2020. Host transcriptional response to persistent infection with a live-attenuated porcine reproductive and respiratory syndrome virus strain. Viruses. 12(8):817. https://doi.org/10.3390/v12080817.


5. Vande Pol, K.D., Tolosa, A.F., Shull, C.M., Brown, C.B., Alencar, S.A., Lents, C.A., Ellis, M. 2021. Effect of drying and/or warming piglets at birth under warm farrowing room temperatures on piglet rectal temperature over the first 24 h after birth. Translational Animal Science. txab060. https://doi.org/10.1093/tas/txab060.


6. Kerns K, Sharif M, Zigo M, Miller D, Sutovsky S. 2020. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. Int J Mol Sci 21:2121.


7. Sharif M, Kerns K, Sutovsky P, Bovin N, Miller. DJ  2021. Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner. Reproduction. 161:449-457.


8. Johnson, JS, JM Maskal, AW Duttlinger, KR. Kpodo, BR McConn, CJ Byrd, BT Richert, JN Marchant-Forde, DC Lay, SD Perry, MC Lucy, TJ Safranski.   In utero heat stress alters the postnatal innate immune response of pigs. J. Anim. Sci. 98 (12):1-13.


9. Bernhard, CJ, KG Sharp, TJ Safranski, WR Lamberson and MC Lucy.   Reproduction and reproductive tract morphology of male and female pigs whose mothers were heat stressed during the second month of gestation.  J. Anim Sci. 98(11): 1-12.


10. Maskal, J.M., AW Duttlinger, KR Kpodo, BR McConn, CJ Byrd, JN Marchant-Forde, DC Lay, SD Perry, MC Lucy, TJ Safranski, JS Johnson.   Evaluation and mitigation of the effects of in utero heat stress on piglet growth performance, postabsorptive metabolism, and stress response following weaning and transport.  J. Anim. Sci., 98(9):1-13.


11. Ciccarelli M, Giassetti MI, Miao D, Oatley MJ, Robbins C, Lopez-Biladeau B, Waqas MS, Tibary A, Whitelaw B, Lillico S, Park CH, Park KE, Telugu B, Fan Z, Liu Y, Regouski M, Polejaeva IA, Oatley JM. Donor-derived spermatogenesis following stem cell transplantation in sterile NANOS2knockout males. Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24195-24204.  PMID: 32929012


12. Park CH, Jeoung YJ, Uh KJ, Park KE, Bridge J, Powell A, Li J, Pence L, Zhang Y, Liu T, Sun H, Gu Y, Shen Y, Wu J, Belmonte J-C, Telugu BP*. Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos. Stem Cell Reports. 2021 Jan 12;16(1):212-223. doi: 10.1016/j.stemcr.2020.11.011. PMID: 33338433


13. Geisert R.D., A.E. Meyer, C.A. Pfeiffer, D.N. Johns, K. Lee, K.D. Wells, T.E. Spencer, and R.S. Prather. 2021. Gene Editing to Investigate Role of Conceptus Factors in Establishment of Pregnancy. Reproduction 161: R79-R88.


14. Pfeiffer C.A., A.E. Meyer, K.E. Brooks, P.R. Chen, J. Milano-Foster, L.D. Spate, J.A. Benne, R. Cecil, M.S. Samuel, L.A. Ciernia, M.F. Smith, K.D. Wells, T.E. Spencer, R.S. Prather, and D. Geisert. 2020. Ablation of conceptus PTGS2 provides a new understanding of early pregnancy events in the pig. Biol Reprod 102:475-488. doi.org/10.1093/biolre/ioz192.


15. Cho J, Uh K, Ryu J, Fang X, Bang S, Lee K. Development of PCR based approach to detect potential mosaicism in porcine embryos. Journal of Animal Reproduction and Biotechnology. 2020 Dec. 35. 4.


16. Uh K, Ryu J, Farrell K, Wax N, Lee K. TET family regulates the embryonic pluripotency of porcine preimplantation embryos by maintaining the DNA methylation level of NANOG. Epigenetics. 2020 Nov;15(11):1228-1242. doi: 10.1080/15592294.2020.1762392. Epub 2020 May 13.


17. Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P (2020) Is the ubiquitin-proteasome system involved in the degradation of porcine beta-microseminoprotein during sperm capacitation? Int. J. Mol. Sci., 21(11):4151.


18. Kerns K, Sharif M, Zigo M, Xu W, Hamilton L, Sutovsky M, Ellersieck M, Drobnis EZ, Oko R, Miller D, Sutovsky P (2020) Sperm cohort-specific zinc signature acquisition and capacitation-induced zinc flux regulate sperm-oviduct and sperm-zona pellucida interactions. Int. J. Mol. Sci., 21(6):2121; Special Issue on Advances in Molecular Regulation of Spermatozoa Function,


19. Zigo M, Maňásková-Postlerová P, Zuidema D, Kerns K, Jonáková V, Tůmová L, Bubeníčková F, Sutovsky P (2020) Porcine model for the study of sperm capacitation, fertilization and male fertility. Cell Tissue Res., 380(2):237-262.


20. Zuidema D, Sutovsky P (2020) The domestic pig as a model for the study of mitochondrial inheritance. Cell Tissue Res., 80(2):237-262. Special Issue on Animal Models,


21. Pfeiffer, C.A., A.E. Meyer, K.E. Brooks, P.R. Chen, J.M. Foster, L.D. Spate, J.A. Benner, R. Cecil, M.S. Samuel, L.A. Ciernia, M.F. Smith, K.D. Wells, T.E. Spencer, R.S. Prather. 2020. Ablation of conceptus PTGS2 provides a new understanding of early pregnancy events in the pig. Biol. Reprod. Doi: 10/.1093/biolre/ioz192 PMID: 31616930, PMCID: PMC7523696.


22. Stoian, A., R.R.R. Rowland, V. Petrovan, M. Sheahan, M.S. Samuel, K.M. Whitworth, K.D. Wells, J. Zhang, B. Beaton, M. Cigan, R.S. Prather. 2020. The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV). Virology. 2020 Feb; 541:136-140. doi: 10.1016/j.virol.2019.12.007. Epub 2019 Dec 24. PMID: 32056711, PMCID: PMC7112016


23. Chen, P.R., L.D. Spate, E.C. Leffeler, J.A. Benne, R.F. Cecil, T.K. Hord, R.S. Prather. 2020. Removal of hypotaurine from porcine embryo culture medium does not impair development of in vitro fertilization or somatic cell nuclear transfer-derived embryos at low oxygen tension. Molecular Reproduction and Development https:/doi: 10.1002/mrd.23393. PMID 32495478, PMCID: PMC7496716.


24. Cecil, R.F., J.A. Benne, P.R. Chen, T.K. Hord, L.D. Spate, M.S. Samuel, R.S. Prather. 2020. Chemical stimulation of hypoxia in somatic cell nuclear transfer donor cells permits metabolic reprogramming and improved SCNT efficiency. Molecular Reproduction and Development https:/doi: 10.1002/mrd.23392 PMID 32558023, PMCID: PMC7496615.


25. Lucas, C.G., A.M. Spate, M.S. Samuel, L.D. Spate, W.C. Warren, R.S. Prather, K.D. Wells. 2020. A novel swine sex-linked marker and its application across different mammalian species. Transgenic Research doi: 10.1007/s11248-020-00204-z. PMID: 32607872, PMCID: PMC7423754


26. Ostedgaard, L.S., M.P. Price, K.M. Whitworth, M.H.A. Alaiwa, A.J. Fischer, A. Warrier, M. Samuel, L.D. Spate, P.D. Allen, B.M. Hilkin, G.S. R. Ibarra, M.E. Ortiz, B.J. Goodelll, S.E. Mather, L.S. Powers, M.R. Stroik, N.D. Gansmer, C.E. Hippee, K. Zarei, J.A. Goeken, T.R. Businga, E. A. Hoffman, D.K. Meyerholz, R.S. Prather, D.A. Stoltz, M.J. Welsh. 2020. Lack of submucosal glands impairs respiratory host defenses in pigs. eLife 2020;9:e59653. DOI: https://doi.org/10.7554/eLife.59653. PMID:33026343, PMCID: PMC7541087


27. Koppes, E., B.K. Redel, M.A. Johnson, K.J. Shvorak, L. Ghaloul-Gonzalez, M.E. Yates, D.W. Lewis, S.M. Gollin, Y. Wu, S.E. Christ, M. Yerle, A. Leshinski, L.D. Spate, J. Benne, S. Murphy, M.S. Samuel, E.M. Walters, S.A. Hansen, K.D. Wells, J. Vockley, R.S. Prather, R.D. Nicholls. 2020. A porcine model of phenylketonuria generated by CRISPR/Cas9 genome editing. JCI Insight doi: 10.1172/jci.insight.141523. PMID: 33055427 PMCID: PMC7605535.

 

Boar Performance

1. Mills, K.M., Aryal, U.K., Sobreira, T., Minton, A.M., Casey, T., and K.R. Stewart. 2020. Shotgun proteome analysis of seminal plasma differentiate boars by reproductive performance.  Theriogenology 157: 130-139.  doi.org/10.1016/j.theriogenology.2020.07.013


2. Lents, C.A., Supakorn, C., DeDecker, A.E., Phillips, C.E., Boyd, R.D., Vallet, J.L., Rohrer, G.A., Foxcroft, G.R., Flowers, W.L., Trottier, N.L., Salak-Johnson, J.L., Bartol, F.F., Stalder, K.J. 2020. Dietary lysine-to-energy ratios for managing growth and pubertal development in replacement gilts. Applied Animal Science. 36(5):701-714. https://doi.org/10.15232/aas.2020-02016. DOI: https://doi.org/10.15232/aas.2020-02016


3. Rempel, L.A., Parrish, J.J., Miles, J.R. 2020. Genes associated with chromatin modification within the swine placenta are differentially expressed due to factors associated with season. Frontiers in Genetics. 11:1019. https://doi.org/10.3389/fgene.2020.01019.


4. Belstra, B.A., Willenburg, K.L., Gómez-López, D.H., Knox, R.V., Stewart, K.R., 2020. Effects of the number of sperm and site of uterine semen deposition on conception rate and the number of embryos in weaned sows receiving a single fixed-time insemination. Journal of Animal Science 98.


5. Kerns K, Jankovitz J, Minton A, Kuster C, Robinson J, Sutovsky P (2020) Relationship between the length of sperm tail mitochondrial sheath and fertility traits in boars used for artificial insemination. Antioxidants, 9(11):1033. doi: 10.3390/antiox9111033


6. Parrish JJ, PREDICITION OF FERTILITY IN MALES, US Patent App. 16/024,335; Patent granted May 2020.

 

Genetics and Genomics

1. Johnson, J.S., Stewart, K.R., Safranski, T.J., Ross, J.W. and L.H. Baumgard.  2020.  In utero heat stress alters postnatal phenotypes in swine.  Theriogenology, 154:110-119. doi.org/10.1016/j.theriogenology.2020.05.013


2. Wijesena, H.R., SD Kachman, CA Lents, JJ Riethoven, MD Trenhaile-Grannemann, TJ Safranski, ML Spangler, DC Ciobanu. Fine mapping genetic variants associated with age at puberty and sow fertility using SowPro90 genotyping array.   J. Anim. Sci. 98(10): 1-12.


3. Johnson, J.S., KR Stewart, TJ Safranski, JW Ross, LH Baumgard. In utero heat stress alters postnatal phenotypes in swine. 2020. Theriogenology, 154: 110-119

 

Sow and Gilt Performance

1. Belstra, B.A., Willenburg, K.L, Gómez-López, D.H., Knox, R.V., and K.R. Stewart. 2020. Effects of number of sperm and site of uterine deposition on conception rate and number of embryos in weaned sows receiving a single fixed time insemination.  J Anim Sci.,98:9, 1-10. doi:10.1093/jas/skaa260


2. Suarez-Trujillo, A., Senn, K., Teeple, K., Casey, T., and K.R. Stewart. 2020. Technical Note: A standardized model to study effects of varying 24 h colostrum dose on postnatal growth and development. TAS, txaa212, doi.org/10.1093/tas/txaa212.


3. Leonard, S.M., Xin, H., Brown-Brandl, T.M., Ramirez, B.C., Johnson, A.K., Dutta, S., Rohrer, G.A. 2021. Effects of farrowing stall layout and number of heat lamps on sow and piglet behavior. Applied Animal Behaviour Science. 239:105334. https://doi.org/10.1016/j.applanim.2021.105334.

 

Teaching and extension

1. Mills, K.M., Schinckel, A.P., Casey, T.M., and K.R. Stewart. 2020. Evaluation of on-farm indicators of gilt reproductive performance potential at 21 days of age.  TAS, txaa210, doi.org/10.1093/tas/txaa210.  

 

 

07/21/2022

Basic Science and Technology

1. Cline, P.M., Tsai, T.C., Lents, C.A., Stelzleni, A.M., Dove, C.R., Azain, M. 2021. Interaction of dietary carbohydrate and fat on glucose metabolism in growing pigs. Domestic Animal Endocrinology. 78. Article 106655. https://doi.org/10.1016/j.domaniend.2021.106655.


2. Meinen, R.J., Spiegal, S.A., Kleinman, P.J., Flynn, K.C., Goslee, S.C., Mikesell, R.E., Church, C., Bryant, R.B., Boggess, M.V. 2022. Opportunities to implement manureshed management in the Iowa, North Carolina, and Pennsylvania swine industry. Journal of Environmental Quality. https://doi.org/10.1002/jeq2.20340.


3. Velez, F.J., Bosilevac, J.M., Singh, P. 2021. Validation of high-resolution melting assays for the detection of virulent strains of Escherichia coli O26 and O111 in beef and pork enrichment broths. Food Control. 128. Article 108123. https://doi.org/10.1016/j.foodcont.2021.108123.

 Boar Performance

1. Andrade, A., Grossfeld, R., Knox, R. V. 2021 In vitro effects of commercial freezing and thawing extenders on boar sperm quality. Anim. Reprod. Sci. Pages 106906 DOI: https://doi.org/10.1016/j.anireprosci.2021.106906.


2. Sharif, M., Hickl, V., Juarez, G., Di, X., Kerns, K., Sutovsky, P., Bovin, N., Miller, D. J.   Hyperactivation is sufficient to release sperm from immobilized oviduct glycans.  Sci Rep 12, 6446. https://doi.org/10.1038/s41598-022-10390-x


3. Sharif, M., Kerns, K., Sutovsky, P., Bovin, N., Miller, D.J.   Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner.  Reproduction. 161(4):449-457. doi: 10.1530/REP-20-0474.

 Sow and Gilt Performance

1. Arend, L.S., Knox, R.V. 2021. Fertility responses of melatonin-treated gilts before and during the follicular and early luteal phases when there are different temperatures and lighting conditions in the housing area. Anim Reprod Sci, 230, 106769. doi:10.1016/j.anireprosci.2021.106769


2. Knox, R.V., Arend, L.S., Buerkley, A.L., Patterson J.L., Foxcroft, G.R. 2021. Effect of physical or fenceline boar exposure and gonadotropins on fertility in gilts. J. Anim. Sci. DOI: 10.1093/jas/skab348.


3. Lindo, A.N., Thorson, J., Bedenbaugh, M.N., Mccosh, R.B., Lopez, J.A., Young, S.A., Meadows, L.J., Bowdridge, E.C., Fergani, C., Freking, B.A., Lehman, M.N., Hileman, S.M., Lents, C.A. 2021. Localization of kisspeptin, NKB, and NK3R in the hypothalamus of gilts treated with the progestin altrenogest. Biology of Reproduction. Article 103. https://doi.org/10.1093/biolre/ioab103.


4. Miles, J.R., Walsh S.C., Rempel L.A., Pannier A.K.. 2022. Mechanisms regulating the initiation of porcine conceptus elongation.  Mol Reprod Dev.   DOI: 10.1002/mrd.23623.


5. Mills, K.M., Ferreira, C.R., Stewart, K.R., Casey T.M.   Biomarkers predictive of long-term fertility found in vaginal lipidome of gilts at weaning.  J Anim Sci DOI: https://doi.org/10.21203/rs.3.rs-210669/v1


6. Mills, K.M., Shirley, L.K., Sharp, K., Garcia, R., Suarez-Trujillo, A., Stewart, K.R. 2021. Effects of induction on the farrowing process and piglet blood parameters at time of birth. TAS 5:108.  doi: 10.1093/tas/txab032 


7. Vande Pol, K.D., Tolosa, A.F., Bautista, R.O., Willard, N.C., Gates, R.S., Shull, C.M., Brown, C.B., Alencar, S.A., Lents, C.A., Ellis, M. 2021. Effects of drying and providing supplemental oxygen to piglets at birth on rectal temperature over the first 24 h after birth. Translational Animal Science. 5(3). Article txab095. https://doi.org/10.1093/tas/txab095.


8. Walsh, S.C., Miles, J.R., Keel-Mercer, B.N., Rempel, L.A., Wright-Johnson, E.C., Lindholm-Perry, A.K., Oliver, W.T., Pannier, A.K. 2022. Global analysis of differential gene expression within the porcine conceptus transcriptome as it transitions through spherical, ovoid, and tubular morphologies during the initiation of elongation. Molecular Reproduction and Development. Article 23553. https://doi.org/10.1002/mrd.23553

 Genetics and Genomics

1. Herrera-Uribe, J., Wiarda, J., Sivasankaran, S.K., Daharsh, L., Liu, H., Byrne, K.A., Smith, T.P., Lunney, J.K., Loving, C.L., Tuggle, C.K. 2021. Reference Transcriptomes of Porcine Peripheral Immune Cells Created Through Bulk and Single-Cell RNA Sequencing. Frontiers in Genetics. 12. Article 689406. https://doi.org/10.3389/fgene.2021.689406.

Teaching and Extension

1. Hines, E., Romoser, M., Cover, J. Improving the gilt selection process.  Penn State Extension Report.  https://extension.psu.edu/improving-the-gilt-selection-process


2. Knox, R. 2021. The New Building Designs on Swine Farms in China. June 2021. Illinois AgriNews.


3. Knox, R. 2021.  New technology used in the swine breeding barn. Dec. 2021. Illinois AgriNews.

 Abstracts/Posters/Book Chapter/Professional Presentations

1. Arend, L.S., Vinas, R.F., Silva, G., Lower, A., Connor, J.F., Knox. R.V.  Effects of nursing a large litter and ovarian response to gonadotropins at weaning on subsequent fertility in first parity sows. Journal of Animal Science, Volume 100, Issue Supplement_2, May 2022, Page 85, https://doi.org/10.1093/jas/skac064.137


2. Hauxwell, K., Ostrand, L.M., Schmidt, T.B., Psota, E., Mote, B.E., Rohrer, G.A. 2021. Activity and bone lesion analysis on gilt retention to the breeding herd [abstract]. Journal of Animal Science. 99(Supplement 1):154. https://doi.org/10.1093/jas/skab054.264.


3. Hines E., Stewart, K.R.   Farrowing induction and your sows.  Extension Webinar.  Penn State Extension.  https://extension.psu.edu/farrowing-induction-and-your-sows-webinar


4. Knox, R. V. 2021. Overview of Management of Reproduction: Pigs. Merck Veterinary Manual. pp 1-22. Management of Reproduction: Pigs - Merck Veterinary Manual (merckvetmanual.com)


5. Knox, R.V. 2021. Gilt puberty, subsequent fertility, and incidence of silent heat in response to boar exposure and hormone induction, A.D. Leman Pre-Conference Workshop.


6. Knox, R.V. 2022. Tools used and needed in the gilt pool. Journal of Animal Science, Volume 100, Issue Supplement_2, May 2022, Pages 9–10, https://doi.org/10.1093/jas/skac064.013


7. Ostrand, L.M., Tranhaile-Grannemann, M.D., See, G., Schmidt, T.B., Psota, E., Rohrer, G.A., Mote, B.E. 2021. Utilization of NUtrack to assess variance components and heritability of activity traits [abstract]. Journal of Animal Science. 99(Supplement 1):17. https://doi.org/10.1093/jas/skab054.030.


8. Parrish, J.J., Gall, T., Wilson, M., Ballard, M. 2022. Salmate fish oil supplement to boars and impact on sperm production and spermatogenesis.


9. Stewart, K.R., Knox, R.V. 2022. Applied animal andrology: Boar in Manual of Animal Andrology, edited by P.J. Chenoweth and S.P. Lorton, CAB International, 2022, pp95-107.


10. Studer, J.M., Kiefer, Z.E., Gianluppi, R., Adur, M.K., Shultz, R.B., Horst, E.A., Mayorga, E.J., Keating, A.F., Baumgard, L.H., Lents, C.A., Ross, J.W. 2021. Direct and indirect effects of heat stress on the hepatic and ovarian proteome in gilts [abstract]. Journal of Animal Science. 99(Supplement 1):106-107. https://doi.org/10.1093/jas/skab054.173.


11. Vinas, R.F., Arend, L.S., Silva, G., Lower, A.J., Connor, J.F., Kaustubh, B., Furugen, A., Andrade, A., Knox. R.V. Evaluation of electrical impedance spectroscopy to determine swine herd reproductive status using the accuracy of ultrasound and hormonal assays as diagnostic tools. Journal of Animal Science, Volume 100, Issue Supplement_2, May 2022, Page 84, https://doi.org/10.1093/jas/skac064.135

 

07/21/2023

Publications:

Basis Science and Technology

1. Li, Y., Adur, M.K., Lonergan, S.M., Keating, A.F., Ross, J.W.   MicroRNA21 inhibition affects porcine oocyte maturation and alters protein expression critical for metabolic pathway function.  Mol Reprod Dev.  89(10):443-458. https://doi:10.1002/mrd.23641.


2. Miller, K.A., Johnson, E.M., Matchan, S.A., Goehring, D., Ross, J.W., Gabler, N.K.   Strategies to manage barn feed supply to prolong and hold late finishing pigs during a supply chain disruption.  Transl Anim Sci. 7(1):txac166. https://doi:10.1093/tas/txac166.


3. Studer, J.M., Schweer, W.P., Gabler, N.K., Ross, J.W.   Functions of manganese in reproduction. Anim Reprod Sci. 238:106924. https://doi:10.1016/j.anireprosci.2022.106924.

 Boar Performance

1. de Andrade, A.F.C., Balogun, K., Machaty, Z., Knox, R.V.  Effects of supplemental antioxidants on in vitro fertility measures for cryopreserved boar spermatozoa. Theriogenology. 200:33-42. https://doi:10.1016/j.theriogenology.


2. Andrade, A.F.C., Knox, R.V., Torres, M.A., Pavaneli, A.P.P.   What is the relevance of seminal plasma from a functional and preservation perspective? Anim Reprod Sci. 246:106946. https://doi:10.1016/j.anireprosci.2022.106946.   


3. Lawlor, M., Zigo, M., Kerns, K., Cho, I.K., Easley, C.A., Sutovsky, P.  Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease. Intl J of Molc Sci. 23(13):7163.  https://doi:10.3390/ijms23137163.


4. Nogueira, E., Tirpák, F., Hamilton, L., Zigo, M., Kerns, K., Sutovsky, M., Kim, J., Volkmann, D., Jovine, L., Taylor, J., Schnabel, R., Sutovsky, P.  A Non-Synonymous Point Mutation in a WD-40 Domain Repeat of EML5 Leads to Decreased Bovine Sperm Quality and Fertility. Front. Cell Dev. Biol.  https://doi:10.3389/fcell.2022.872740.


5. Sharif, M., Hickl, V., Juarez, G., Di, X., Kerns, K., Sutovsky, P., Bovin, N., Miller, D.J.   Hyperactivation is sufficient to release sperm from immobilized oviduct glycans.  Sci Rep.  12:6446. https://doi.org/10.1038/s41598-022-10390-x.


6. Spooner-Harris, M., Kerns, K., Zigo, M., Sutovsky, P., Balboula, A., Patterson, A. A re‑appraisal of mesenchymal‑epithelial transition (MET) in endometrial epithelial remodeling. Cell Tissue Res. 391(2):393-408. https://doi:10.1007/s00441-022-03711-z.


7. Zigo, M., Kerns, K., Sen, S., Essien, C., Oko, R., Xu, D., Sutovsky, P.  Zinc is a master-regulator of sperm function associated with binding, motility, and metabolic modulation during porcine sperm capacitation. Commun. Biol. 5(1):538.  https://10.1038/s42003-022-03485-8.

 Sow and Gilt Performance

1. Arend, L.S., Vinas, R.F., Silva, G.S., Lower, A.J., Connor JF, Knox RV.   Effects of nursing a large litter and ovarian response to gonadotropins at weaning on subsequent fertility in first parity sows. J Anim Sci. 101:skac398. https://doi:10.1093/jas/skac398.


2. Bhatia, V., Stevens, T., Derks, M.F.L., Dunkelberger, J., Knol, E.F., Ross, J.W., Dekkers, J.C.M.   Identification of the genetic basis of sow pelvic organ prolapse.  Front Genet.  14:1154713.  https://doi:10.3389/fgene.2023.1154713.


3. Walsh, S.C., Miles, J.R., Broeckling, C.D., Rempel, L.A., Wright-Johnson, E.C., Pannier, A.K.  Secreted metabolome of porcine blastocysts encapsulated within in vitro 3D alginate hydrogel culture systems undergoing morphological changes provides insights into specific mechanisms involved in the initiation of porcine conceptus elongation. Reprod Fertil Dev. 35(5):375-394. https://doi.org/10.1071/RD22210.

 Seasonality and Heat Stress

1. Adur, M.K., Seibert, J.T., Romoser, M.R., Bidne, K.L., Baumgard, L.H., Keating, A.F., Ross, J.W.   Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period.  J Anim Sci. 100(7):skac129. https://doi:10.1093/jas/skac129.


2. Brown-Brandl, T.M., Hayes, M.D., Rohrer, G.A., Eigenberg, R.A.  Thermal comfort evaluation of three genetic lines of nursery pigs using thermal images. Biosystems Engineering. 225:1-12. https://doi.org/10.1016/j.biosystemseng.2022.11.002.


3. Raber, V.L., Pritchett, R.K., Robbins, L.A., Stewart, K.R., Gaskill, B.N., Green-Miller, A.R., Johnson, J.S.  Evaluating the temperature preferences of sexually mature Duroc, Landrace, and Yorkshire boars.  Transl Anim Sci.  May 29;7(1):txad060. https://doi:10.1093/tas/txad060.


4. Ramirez, B.C., Hoff, S.J., Hayes, M.D., Brown-Brandl, T.M., Harmon, J.D., Rohrer, G.A. 2022.  A review of swine heat production: 2003 to 2020. Frontiers in Animal Science. 3. Article 908434. https://doi.org/10.3389/fanim.2022.908434.


5. Roach, C.M., Bidne, K.L., Romoser, M.R., Ross, J.W., Baumgard, L.H., Keating, A.F.  Impact of heat stress on prolactin-mediated ovarian JAK-STAT signaling in postpubertal gilts.  J Anim Sci. 100(7):skac129. https://doi:10.1093/jas/skac129.


6. Romoser, M.R., Bidne, K.L., Baumgard, L.H., Keating, A.F., Ross, J.W.   Effects of increased ambient temperature and supplemental altrenogest before pregnancy establishment in gilts. J Anim Sci. 100(2):skac007. https://doi:10.1093/jas/skac007.


7. Rudolph TE, Roach CM, Baumgard LH, Ross JW, Keating AF, Selsby JT.   The impact of Zearalenone on heat-stressed skeletal muscle in pigs.  J Anim Sci. 100(8):skac215. https://doi:10.1093/jas/skac215.

 Abstracts/Posters/Professional Presentations

1. Geisert, R. Gene editing provides a tool to investigate the role of conceptus factors involved in the establishment of pregnancy.  Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)


2. Kerns, K.   Capacitation Patterns.  Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)


3. Knox, R.   Follicle Development in Pigs:  State of the Art.  Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)


4. Loucks, A., Hines, E.A.   Processed soy ingredients may adequately replace soybean meal in sow transition diets.  Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium.  Abst. 36. (Poster & Flash talk)


5. Miles, J.   Mechanisms regulating the initiation of porcine conceptus elongation. Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)


6. Miles, J.R., Rempel, L.A., Snider, A.P., Pannier, A.K. 2023 Characterization of mRNA expression for key glycerophospholipid and sphingolipid enzymes within trophectoderm and embryonic disc during initiation of porcine conceptus elongation. Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium.  57. (Poster) 


7. Nordell*, N.E., C.J. Bernhard, M. Wegner*, R. Metcalf*, J. Rupert*, D. Lagundžin, Sharma, K.G. Sharp, T.J. Safranski, M.C. Lucy, Desaulniers, A.T. 2023. In utero exposure to maternal heat stress impairs Leydig cell development and function in the boar. ASAS Midwest Animal Science. Madison, WI. (Oral presentation)


8. Nordell, N.E., C.J. Bernhard, M. Wegner, R. Metcalf, J. Rupert, D. Lagundžin, K. Sharma, K.G. Sharp, T.J. Safranski, M.C. Lucy, Desaulniers, A.T. 2022. In utero heat stress impairs Leydig cell development and function in a porcine model. 19th Annual Gilbert S. Greenwald Symposium on Reproductive and Developmental Sciences, Kansas City, MO. Abst 13.  (Poster)


9. Parrish, J.J., Gall, T., Wilson, M., Parsley, M., Ballard, M.   Protected fish oil supplement to boars and the impact on sperm production and spermatogenesis.  ASAS Midwest Animal Science. Madison, WI.  (Oral presentation)


10. Studer, J., Kiefer, Z.E., Koester, L., Johnson, E., Baumgard, L.H., Schmitz-Esser, S., Greiner, L.L., Keating, A.F., Farkas, A., Galina-Pantoja, L., Vonnahme, K.A., Ross, J.W.   Metabolites in Insulin Resistance and Metabolic Syndrome Altered in Sows Suffering from Postpartum Dysgalactia Syndrome.  Proceedings of the Society for the Study of Reproduction 56^th Annual Meeting, Ottawa, Canada.  Abst P489 (Poster) 


11. Taechamaeteekul, P., Sang-Gassanee, K., Lin H.Y., Collell, M., Komolpis, K., Knox, R.V., Tummaruk, P. 2023 A clinical application of serum progesterone strip test to determine reproductive status in replacement gilts. Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium. 28. (Poster)


12. White, B.   Gonadal steroidogenesis in boars and gilts with reduced endogenous levels of GnRH-II receptor.  Proceedings of the 11^th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)


13. Zigo, M., Kerns, K., Ahlering, P., Sutovsky, P.   Comparison of Human and Porcine Sperm Zincoproteomes with Focus on Clinical and Evolutionary Implications.  Proceedings of the Society for the Study of Reproduction 56^th Annual Meeting, Ottawa, Canada.  Abst P456 (Poster)  

Multi-station Funded Grants (Active):

1. NIH, R01HD095841, “Accumulation, Storage, and Release of Sperm in the Oviduct” Miller¹, D. (PD), Prather², R,. Sutovsky², P. (¹University of Illinois; ²University of Missouri)


2. USDA-AFRI-NIFA Grant #2021-67015-34416, $500,000, Jul 1, 2021 – Oct 1, 2024, “Influence of Maternal and Embryonic Extracellular Vesicles on the Initiation of Porcine Conceptus Elongation” Pannier¹, A.K. (PD), Miles², J.R. (Co-PD), Rempel², L.A. (¹University of Nebraska; ²USDA, ARS, USMARC)


3. USDA-AFRI-NIFA Grant #2022-08916, $999,618, May 1, 2023 – May 1, 2027, “Identifying AMR gene reservoirs and bacterial host-AMR gene associations to identify bacterial host range of AMR genes in swine production systems” Fernando¹, S., Burkey¹, T., Miller¹, P., Schmidt², A., Anderson², C., Oliver³, W., Rempel³, L., Wells³, J. (¹University of Nebraska; ²Iowa State University; ³USDA, ARS, USMARC)


4. USDA-AFRI-NIFA Grant #2023-XXXXX, $650,000, May 1, 2023 – May 1, 2026, “Enhancing boar fertility in the face of climate change through the mitigation of in utero heat stress” Desaulniers¹, A.T. (PD), Lents², C., White¹, B., Brito³, L, Johnson⁴, J. (¹University of Nebraska; ²USDA, ARS, USMARC, ³Purdue University; ⁴USDA, ARS, West Lafayette)