NCERA_old57: Swine Reproductive Physiology

(Multistate Research Coordinating Committee and Information Exchange Group)

Status: Active

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SAES-422 Reports

Annual/Termination Reports:

[07/20/2020] [07/12/2021] [07/21/2022] [07/21/2023]

Date of Annual Report: 07/20/2020

Report Information

Annual Meeting Dates: 05/19/2020 - 05/19/2020
Period the Report Covers: 10/01/2019 - 09/30/2020

Participants

Participant List (only include members and visitors that attended the annual meeting)
Geisert, Rod; University of Missouri
Hamernik, Deb; University of Nebraska-Lincoln
Hines, Elizabeth A; Penn State University
Feugang, Jean M.N.; Mississippi State University
Knox, Rob; University of Illinois
Lugar, Drew; Illinois State University
Maddox, Carol; University of Illinois
Miles, Jeremy; USMARC
Miller, David; University of Illinois
Parrish, John; University of Wisconsin
Rempel, Lea; USMARC
Ross, Jason; Iowa State University
Safranski, Tim; University of Missouri
Stewart, Kara; Purdue University
White, Brett; University of Nebraska-Lincoln

Visitors:
Hamilton, Christina; NCERA Assistant Director
Mathew, Alan; Purdue Animal Science Department Head
Minton, Amanda; The Mashoffs

Brief Summary of Minutes

Brief Summary of Minutes of Annual Meeting

Meeting called to order on Tuesday, May 19^th at 9:00 am CST via WebEx by institutional host, Dr. Kara Stewart. Business meeting immediately commenced. Due to the COVID19 pandemic, the scheduled symposium was postponed until next year and it was decided that it would be held mid-May of 2021 at Purdue University. The committee members thanked Kara Stewart for organizing the symposium and are looking forward to the symposium next year.

Recruitment was discussed, Brett White agreed to reach out to Amy Schweitzer as potential new member. Others agreed to consider potential new members and reach out.

John Parrish will serve as secretary for 2021, as self-nominated and approved by committee members. Chair for 2021 will be Elizabeth Hines, as promoted from current secretary.

Extensive discussion was held among members regarding current state of the swine industry due to COVID19, with updates provided from Indiana, Missouri, Pennsylvania, and Iowa.

It was brought up that member Benny Mote was unable to attend due to family emergency, Kara Stewart offered to organize a card from the committee for Benny.

Meeting proceeded with update from Christina Hamilton. Brief notes: Chris encouraged the use of the North Central Regional Association (NCRA) State Ag Experiment Station Directors website (www.ncra-saes.org) as well as the use of impact writing resources for the group. These resources likely improve efficiency of reporting and in acquiring grant funding for the group. Brief overview of the Multistate Research System: Funded from the Hatch Act, at least 25% of Hatch funds need to be spent on multi-state projects. Project committees, organized through NIMSS, goal is to focus on multi-state collaborations in specified research areas. NCERA57 focus on swine reproductive physiology research, as well as dissemination of that research. North Central virtual Handbook: Guidelines for Multistate Research Activites, provides instructions for developing annual reports. Renewed emphasis from NIFA for ‘shorter reports that really showcase multistate portfolio and emphasize importance of work to stakeholders’. Reports due 60 days after annual meeting. Utilize template that was circulated for building station reports. Highly encouraged us to look into visiting with Multistate Research Fund Impacts (site) for assistance in writing powerful impact statements through workshop at our next meeting.

Station reports on research were given by the following parties: Brett White; Kara Stewart; Jeremy Miles; Rod Geisert; Guest- Amanda Minton; Elizabeth Hines; Jason Ross; Dave Miller; Rob Knox; John Parrish.

Discussion on current teaching climate was initiated by John Parrish regarding student and educational transition from in-person to online classes. Due to yet unknown circumstances with education and progression of COVID19 pandemic, open source online learning resources were discussed. Encourage collaboration on materials with possibility of restricted in-person lectures. If any insight on keeping students on task or aid in online classroom learning, please share. Rod Geisert and John Parrish offered to share video resources with the committee.

Discussion of pursuing funding sources was held, review of current needs of the swine industry in COVID19 and how experts on the team can best serve those needs. Some focus recommended to be placed on resiliency of food system as well as precision agriculture. Will revisit at a later date.

Adjourn 4:35 pm, CST.

Accomplishments

Accomplishments Supporting Objectives <ul> <li>In support of Objective 1, researchers determined boars with varicocele had decreased semen quality during and after a heat stress event (USMARC and UNL ). </li> <li>In support of Objective 2 and 4, investigators reported on pubertal neuroendocrine development in gilts (USMARC and WVU ).</li> <li>In support of Objective 3 and 4, a team of researchers evaluated the metabolite profile of uterine flushings during early embryo elongation in swine (USMARC and UNL ).</li> <li>In support of Objective 3 and 5, SowBridge (IPIC) talk on Dealing with Delayed Puberty in Gilts. Expert Panel: R Knox, K. Stewart, and T. Safranski. SowBridge. Aug. 7, 2019.(Iowa State, Univ. Illinois, Purdue, Univ. Missouri). </li> <li>In support of Objective 1 and 4, researchers identified potential biomarkers in sperm that may provide early indication of fertility reduction due to elevated environmental temperature (Univ. of Illinois, Purdue, Univ. Wisc). </li> <li>In support of Objective 1 and 4, researchers developed predictors and mediation methods for sperm quality in agricultural species (Univ Wisc, Miss. State).</li> <li>In support of Objective 1 and 3, researchers collaborated with industry on the implications of low-dose semen (Purdue, Univ. of Illinois). </li> <li>In support of Objective 3, Experts hosted an all-day workshop at Purdue University for heritage Large Black swine breeders (Purdue, Univ. of Missouri). </li> <li>In support of Objective 1, researchers validated a novel microscopic imaging approach for high-throughput evaluation of sperm quality (MSSTATE and University of Illinois, UC publication) and demonstrated the usefulness of nanotechnology-based approaches to improving sperm quality (MSSTATE and Wisconsin University).</li> <li>In support of Objective 1, investigators from different stations examined the mechanisms underlying LH-independent testosterone production. (UNL)</li> <li>In support of Objective 1 and 3, collaborators evaluated sperm kinematics in semen from GnRH-II receptor knockdown and littermate control boars. (UNL)</li> <li>In support of Objective 2, research stations collaborated to compare endocrine profiles of GnRH-II receptor knockdown vs. littermate control gilts. (UNL)</li> <li>In support of Objective 2 and 5, researchers created of vulva scoring scale and industry wide preliminary investigation into pelvic-organ-prolapse (POP) among sows(ISU)</li> <li>In support of Objective 2 and 4, researchers evaluated the impact of heat stress, and potential heat-stress mitigation, on ovarian function and reproductive success (ISU)</li> </ul>

Publications

Publications Basic Science and Technology <ol> <li>Gruhot, T.R., Rempel, L.A., White, B.R., Mote, B.E. 2020. The effect of varicocele on semen quality in boars exposed to heat stress. Translational Animal Science. 4(1):1-6. https://doi.org/10.1093/tas/txaa003.</li> <li>Kerns K, Sharif M, Zigo M, Miller D, Sutovsky S. 2020. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. Int J Mol Sci 21:2121.</li> <li>Rubessa, M., Feugang, J.M., Kandel, M.E., Schreiber, S., Hessee, J., Salerno, F., Meyers, S., Chu, I., Popescu, G., Wheeler, M.B. 2020. High-throughput sperm assay using label-free microscopy: morphometric comparison between different sperm structures of boar and stallion spermatozoa. Animal Reproduction Science. 106509. <a href="https://doi.org/10.1016/j.anireprosci">https://doi.org/10.1016/j.anireprosci</a>.</li> <li>Sharif M, Kerns K, Sutovsky P, Bovin N, Miller. DJ Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner. Submitted.</li> <li>Walsh, S.C., Miles, J.R., Yao, L., Broeckling, C.D., Rempel, L.A., Wright-Johnson, E.C., Pannier, A.K. 2020. Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation. Journal of Molecular Reproduction and Development. 87:174-190. <a href="https://doi.org/10.1002/mrd.23306">https://doi.org/10.1002/mrd.23306</a>.</li> <li>Boettcher AN, Kiupel M, Adur MK, Cocco E, Santin AD, Bellone S, Charley SE, Blanco-Fernandez B, Risinger JI, Ross JW, Tuggle CK, Shapiro EM. Human Ovarian Cancer Tumor Formation in Severe Combined Immunodeficient (SCID) Pigs. Front Oncol. 2019 Jan 22;9:9. doi: 10.3389/fonc.2019.00009. eCollection 2019. PMID: 30723704.</li> <li>Schultz, R., Serao, N.L., Ross, J.W. 2019. Genetic improvement of livestock, from conventional breeding to biotechnological approaches. In: Animal Agriculture:Sustainability, Challenges and Innovations. Eds. Bazer, F.W., Wu, G. In Press (Elsevier).</li> <li>Mayorga E.J., Renaudeau, D., Ramirez, B.C., Ross, J.W., Baumgard, L.H. 2019. Heat stress adaptation in pigs. Animal Frontiers 9:54-61.</li> </ol> Boar Performance <ol> <li>Feugang JM, Rhoads CE, Mustapha PO, Tardif S, Parrish JJ, Willard ST, Ryan PL. 2019. Treatment of boar sperm with nanoparticles for improved fertility. Theriogenology 137:75-81.</li> <li>Hufana-Duran D, Duran PG, Venturina E, Peralta MD, Parrish JJ. 2019. Predicting bull fertility by sperm nuclear shape in water buffalo (bubalus bubalis). Thai J. Vet Med 49:75-81.</li> <li value="5">Lugar, D.W., Harlow, K.E., J. Hundley, M. Goncalves, J. Bergstrom, and K.R. Stewart. 2019. Effects of increased levels of supplemental vitamins during the summer in a commercial AI boar stud. Animal 1-13. doi:10.1017/S1751731119001150</li> </ol> Genetics and Genomics <ol> <li>Wijesena, H.R., Rohrer, G.A., Nonneman, D.J., Keel, B.N., Petersen, J.L., Kachman, S.D., Ciobanu, D.C. 2019. Evaluation of genotype quality parameters for SowPro90, a novel genotyping platform for swine. Journal of Animal Science. 97(8):3262-3273. <a href="https://doi.org/10.1093/jas/skz185">https://doi.org/10.1093/jas/skz185</a>.</li> <li>Lents, C.A., Lindo, A.N., Hileman, S.M., Nonneman, D.J. 2020. Physiological and genomic insight into neuroendocrine regulation of puberty in gilts. Domest Anim Endocrinol. 11:106446. doi: 10.1016/j.domaniend.2020.106446.</li> </ol> Sow and Gilt Performance <ol> <li>Harlow, K., Ferreira, C.R., Sobreira, T.J.P., Casey, T. and K.R. Stewart.   Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet. PLoS ONE 14(9): e0215186. https://doi.org/10.1371/journal.pone.0215186</li> <li>Harlow, K., Suarez-Trujillo, A., Hedrick, V., Sobreira, T.J.P., Aryal, U.K., Stewart, K.R. and T. Casey. Temporal analysis of vaginal proteome reveals developmental changes in lower reproductive tract of gilts across the first two weeks of postnatal.  Sci Rep. 2019 Sep 13;9(1):13241. doi: 10.1038/s41598-019-49597-w. </li> <li>Leonard, S.M., Xin, H., Brown-Brandl, T.M., Ramirez, B.C., Dutta, S., Rohrer, G.A. 2020. Effects of farrowing stall layout and number of heat lamps on sow and piglet production performance. Animals. 10:348. <a href="https://doi.org/doi:10.3390/ani10020348">https://doi.org/doi:10.3390/ani10020348</a>.</li> <li>Seibert, J.T., Adur, M.K., Schultz, R.B., Thomas, P.Q., Kiefer, Z.K., Keating, A.F., Baumgard, L.H., Ross, J.W. 2019. Differentiating between the effects of heat stress and lipopolysaccharide on the porcine ovarian heat shock protein response. J Anim Sci. 97:4965-4973. doi: 10.1093/jas/skz343. PMID: 31782954.</li> <li>Bidne KL, Romoser MR, Ross JW, Baumgard LH, Keating AF. 2019. Heat stress during the luteal phase decreases luteal size but does not affect circulating progesterone in gilts1. J Anim Sci. 97:4314-4322. doi: 1093/jas/skz251.</li> <li>Hines EA, Romoser MR, Kiefer ZE, Keating AF, Baumgard LH, Niemi J, Gabler NK, Patience JF, Haberl B, Williams NH, Kerr BJ, Touchette KJ, Ross JW. 2019. The impact of dietary supplementation of arginine during gestation in a commercial swine herd: I. Gilt reproductive performance. J Anim Sci. 97:3617-3625. doi: 10.1093/jas/skz233.</li> </ol> Teaching and Extension <ol> <li>Boyd, R., Zier-Rush, C., Moeser, A., Culbertson, M., Stewart, K., Rosero, D., & Patience, J. 2019. Review: Innovation through research in the North American pork industry. Animal, 13(12), 2951-2966. doi:10.1017/S1751731119001915</li> </ol>   Abstracts/Posters/Professional Presentations <ol> <li>Adur, M.K., Romoser, M.R., Bidne, K.L., Schultz, R.B., Keating, A.F., Baumgard, L.H., Ross, J.W. 2019 Proteomic assessment of the intra-uterine secretome during the peri- implantation phase to understand effects of increased ambient temperature on the porcine heat stress 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.</li> <li>Corredor, F.A., Sanglard, L.P., Ross, J.W., Keating, A.F., Serao, N.L. 2019. Phenotypic and genomic relationships between vulva score and reproductive performance in first- parity sows. American Society of Animal Science Midwest Section Meeting. Omaha, March 11-13.</li> <li>Desaulniers, A., Cederberg, R., Knox, R., Lents, C.A., White, B. 2019. Use of genetic engineering to elucidate reproductive physiology of the boar. In: Missouri Physiological Society 2019 Annual Meeting Abstracts, 26Oct2019, Columbia, MO. pp. 6.</li> <li>Doungkamchan K, Parrish JJ, Miller DJ. 2020. Proteomic identification of molecular pathways and biomarkers related to porcine seasonal heat-induced infertility in males. 53rd Annual Meeting of the Society for the Study of Reproduction.</li> <li>Ebrecht, M.A., Ross, C.E., Cederberg, R.A., Lovercamp, K.W., Desaulniers, A.T., White, B.R. 2019. Effects of GnRH-II on spermatogenic function of swine. Proceedings of the 16th Annual Gilbert S. Greenwald Symposium on Reproduction and Perinatal Research. p. 44.</li> <li>Kerns K, Sharif M, Zigo M, Miller DJ, Sutovsky P. 2019. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. 52th Annual Meeting of the Society for the Study of Reproduction.</li> <li>Kiefer, Z.E., Chipman, A.L., Studer, J., Johnson, C., Rademacher, C.J., Linhares, D.C.L., Ross, J.W. 2019. Update on Prolapses and Pig Survivability Initiative. James D. McKean Swine Disease Conference. Ames, Iowa. November 7-8.</li> <li>Kiefer, Z.E., Chipman, A.L., Studer, J.M., Koester, L.R., Showman, L., Keating, A.F., Schmitz-Esser, , Ross, J.W. 2019. Identification of putative factors associated with pelvic organ prolapse in sows during late gestation. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.</li> <li>Knox RV, Belstra BA, Willenburg KL, D Gómez-López DH, and Stewart KR.   Effects of number of sperm and site of uterine semen deposition on conception rate and number of embryos in weaned sows receiving a single fixed time insemination. IXth International Conference on Boar Semen Preservation. Sydney, Australia</li> <li>Mills, K., U.K. Aryal, T. Sobreira, A.M. Minton, T. Casey, K.R. Stewart. 2020. Shotgun proteomics reveal seminal plasma proteomes are reflective of boar reproductive performance. 2020 Midwest ASAS.</li> <li>Park, S.B., Gruhot, T.R., Liao, S.F., Rude, B.J., Mote, B.E., Feugang, J.M. 2019. Favorable seminal plasma environment to sperm fertility after L-arginine addition to boar diet. 42nd International Conference of Boar Semen Preservation (ICBSP), Hunter Valley, NSW, Australia.</li> <li>Rohrer, G.A., Cross, A.J., Keel, B.N., Brown-Brandl, T.M., Cassady, J.P. 2019. Genetic factors associated with changes in feeding behavior due to elevated temperature. In: Proceedings of the Plant and Animal Genome Conference, San Diego, CA, Jan. 12-15, 2019. pg. 8, W034. Available: <a>https://www.intlpag.org/2019/images/pdf/2019/PAGXXVII-abstracts-workshops.pdf</a></li> <li>Ross, C.E., Choat, F.H., Plager, K.N., Desaulniers, A.T., Cederberg, R.A., Mills, G.A., White, B.R. 2019. Effect of GnRH-II and its receptor on boar semen quality. Reproduction, Fertility and Development. 32(2):201. <a href="https://doi.org/10.1071/RDv32n2Ab53">https://doi.org/10.1071/RDv32n2Ab53</a>.</li> <li>Ross, J.W., Hale, B.J., Seibert, J.T., Adur, M.K., Keating, A.F., Baumgard, L.H. 2019. Physiological mechanisms through which heat stress compromises reproduction in pigs. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.</li> <li>Schultz, R.B., Bidne, K.L., Romoser, M.R., Adur, M.K., Baumgard, L.H., Keating A.F., Ross, J.W. 2019. Effect of heat stress on the corpus luteum proteome during early pregnancy establishment in pigs. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.</li> <li>Sharp, K., Mills, K., Garcia, R., K.R. Stewart.   Impacts of various farrowing induction protocols in attended farrowings.  Midwest ASAS, Omaha, NE.</li> <li>Shirley, L.K., L.K. Shirley, M. Burgett,A.P. Schinckel, T. Field, R. Garcia, J.S. Johnson, K.R. Stewart, R.M. Stwalley. Evaluations of cooling pads on late lactation sows.  Midwest ASAS, Omaha, NE.</li> <li>Stewart, K.R., T. Casey, K. Mills, U.K. Aryal and A.M. Minton. Shotgun proteome anaylsis of seminal plasma proteins differentiate boars by reproductive performance.  ICBSP, Hunter Valley, Australia.</li> <li>Studer, J.M., Kiefer, Z.E., Keating, A.F., Baumgard, L.H., Olsen, K.M., Rambo, Z., Wilson, M.E., Rapp, C., Ross, J.W. 2019. Investigating the impact of manganese supplementation on corpus luteum function. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.</li> <li>Supakorn, C., Lents, C.A., Martinez, X., Vallet, J.L., Boyd, R.D., Rohrer, G.A., Dedecker, A.E., Stalder, K.J. 2019. Associations between day one piglet serum immunocrit ratio and subsequent growth during gilt development [abstract]. Journal of Animal Science. 97 (Supplement 2):146. Abstract 352. <a href="https://doi.org/10.1093/jas/skz122.258">https://doi.org/10.1093/jas/skz122.258</a>.</li> <li>Walsh, S., Miles, J.R., Wright-Johnson, E.C., Keel, B.N., Rempel, L.A., Pannier, A.K. 2019. Transcriptomic profiles of uniform populations of in vivo-produced spherical, ovoid, or tubular porcine embryos during the initiation of elongation. Reproduction, Fertility and Development. 32(2):152. <a href="https://doi.org/10.1071/RDv32n2Ab53">https://doi.org/10.1071/RDv32n2Ab53</a>.</li> </ol>

Impact Statements

• Enhance development of novel technologies for sperm selection to improve male fertility.

Date of Annual Report: 07/12/2021

Report Information

Annual Meeting Dates: 05/17/2021 - 05/18/2021
Period the Report Covers: 10/01/2020 - 09/30/2021

Participants

Elizabeth HInes, Penn State University;
John Parrish, University of Wisconsins-Madison;
Andre Furugen, University of Illinois;
Rod Geisert, University of Missouri;
Dave Miller, University of Illinois;
Adele Turzillo, Penn State University;
Bret White, University of Nebraska-Lincoln;
Jeremy Miles, USDA, MARC;
Kara Stewart, Purdue University;
Lea Rempel, USDA, MARC ;
Lee Johnston, University of Minnesota;
Lidia Sbaraini Arend, University of Illinois;
Rob Knox, University of Illinois;
Raquel Fernandez, University of Illinois;
Carol Madox, University of Illinois;
Mark Mirando, USDA;
Jean Feugang, Mississippi State University;
Jason Ross, Iowa State University;

Brief Summary of Minutes

The meeting was called to order at 1:00 pm by Elizabeth Hines via a zoom connection. We were welcomed by Adele Turzillo, Department head at Penn State Department of Animal Sciences who described the campus and overview of the department.

John Parrish presented an opportunity to the group to submit a special collection on Pig Reproduction and the history of the committee to the Journal of Animal Science. He is also now the Editor and Chief of the Image Gallery for ASAS and welcomed people to submit images and videos for teaching.

Station Reports: Wisconsin by John Parrish; Nebraska by Brett White, Missouri - Rod Geisert; Illinois - Rob Knox, Purdue - Kara Stewart, Penn State - Elizabeth Hines;

Lastly for the first day Troy Ott and Joy Pate discussed the Center for reproductive biology and health at Penn state. It is cross campus, have a group meeting every other week with ideas on grants wth critic and comments given. They also have journal clubs for graduate students and post docs. Has physical facilites with group shared equipment and lab space.

The meeting then came to an end for Monday May 17.

The meeting resummed on May 18 at 9:00.

Station Reports: MARC Nebraska - Lea Rempel; Iowa State University - Jason Ross

Buisness meeting:

Business meeting started at 10 AM. The symposium was a no go this year and so will be next year, 2022 on "Managing the Highly Prolic Sow." The motion was moved and seconded and approved. The comitted for the symposium is Kara Stewart, Tim Safranski, Lea Rempel and Jason Ross. It was suggested that a component of the symposium should be to go over basics of reproductive management, such as why and how to use the basics of pg600 and matrix etc. The symposium will be on May 24, 2022, Tuesday with some station reports to follow and finish with a half day of buisness meeting and reports on May 25, 2022. Will have a opening event at Fair Oaks on Monday evening, May 23, 2022.

Mark Mirando next presented the NIFA report and the new administrator changes at USDA.

Recruitment was next discussed and Amy Switzer Desaulers at UNL Vet Science was suggested as a target. This was followed by John Parrish discussing further the opportunity for a special collection in Journal of Animal Science. The committee then thanked Elizabeth Hines for hosting the on line meeting. Lastly Jeremy Miles was appointed as next years secretary.

Station Reports: Illinois - Dave Miller; Other stations had nothing to report

Many thanks were expressed to Elizabeth Hines for chairing the committee and hosting the meeting.

Overall it was noted that Covid had major impacts on the ability of the comittee to work with other members on research in the last year and it is expected to improve in the next year.

John Parrish was moved from Secretary to Chair of the committee and the meeting was adjourned at 11:30 AM.

Accomplishments

Accomplishments supporting objectives: <ol> <li>Objective 1, 3 and 4 - Impact of heat stress on the boar and impact on placental gene expression (USDA-MARC, Wisconsin)</li> <li>Objective 1 and 4 - developed predictors and mediation methods for sperm quality in agricultural species (Wisconsin, Miss. State).</li> <li>Objective 2 and 4 - managing growth and puberty development in replacement gilts (USDA-MARC, NC)</li> <li>Objective 2 and 4 - mapping genetic variants associated with age at puberty and sow fertility (USDA-MARC, Missouri)</li> <li>Objective 3 - GnRH2 in the boar (UNL, USDA-MARC</li> <li>Objective 3 - GnRH2 in the gilt ovary (UNL, USDA-MARC)</li> <li>Objective 2 and 4- effects of heat stress on the hepatic and ovarian proteome in gilts (Iowa State, USDA-MARC)</li> <li>Objective 5 - Extension program targeted to translating science into usable information for swine farms (Purdue, Missouri, Illinois)</li> <li>Objective 3 - Investigating survivability in swine (Purdue, Iowa)</li> <li>Objective 1, 3 and 4 - determined the effects on the sperm proteome of a mild acute increase in scrotal temperature in pigs with over 800 proteins identified (Illinois, Wisconsin)</li> <li>Objective 1, 3 and 4 - identified 4 potential biomarkers found in sperm that may provide early indication of fertility reduction due to elevated environmental temperature (Illinois, Wisconsin</li> <li>Objective 1 - Number of sperm in AI and impacts on fertilization (Illinois, Purdue, Wisconsin)</li> <li>Objective 2 - In utero heat stress alters postnatal phenotypes in swine (Missouri, Purdue, Iowa)</li> <li>Objective 3 and 4 - Gene Editing to Investigate Role of Conceptus Factors in Establishment of Pregnancy (Missouri, Tx)</li> <li>In support of Objective 1, researchers validated a novel microscopic imaging approach for high-throughput evaluation of sperm quality (MSSTATE and University of Illinois) and demonstrated the usefulness of nanotechnology-based approaches to improving sperm quality (MSSTATE and Wisconsin University).</li> </ol>

Publications

Basic Science and Technology <ol> <li>Wijesena, H.R., Kachman, S.D., Lents, C.A., Riethoven, J.J., Trenhaile-Grannemann, M.D., Safranski, T.J., Spangler, M.L., Ciobanu, D.C. 2020. Fine mapping genetic variants associated with age at puberty and sow fertility using Sowpro90 genotyping array. Journal of Animal Science. 98(10):1-12. <a href="https://doi.org/10.1093/jas/skaa293">https://doi.org/10.1093/jas/skaa293</a>.</li> <li>Azain, M., Cline, P.M., Tsai, T.C., Lents, C.A., Stelzleni, A.M., Dove, C.R. 2020. Interaction of dietary carbohydrate and fat on glucose metabolism in growing pigs. Research Square. pp. 22. <a href="https://doi.org/10.21203/rs.3.rs-48869/v1">https://doi.org/10.21203/rs.3.rs-48869/v1</a>.</li> <li>Nastasijevic, I., Schmidt, J.W., Boskovic, M., Glisic, M., Kalchayanand, N., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M., Bosilevac, J.M. 2020. Seasonal prevalence of Shiga toxin-producing Escherichia coli on pork carcasses for three steps of the harvest process at two commercial processing plants in the United States. Applied and Environmental Microbiology. 87(1):e01711-20. <a href="https://doi.org/10.1128/AEM.01711-20">https://doi.org/10.1128/AEM.01711-20</a>.</li> <li>Chaudhari, J., Liew, C., Workman, A.M., Riethoven, J.M., Steffen, D., Sillman, S., Vu, H.L.X. 2020. Host transcriptional response to persistent infection with a live-attenuated porcine reproductive and respiratory syndrome virus strain. Viruses. 12(8):817. <a href="https://doi.org/10.3390/v12080817">https://doi.org/10.3390/v12080817</a>.</li> <li>Vande Pol, K.D., Tolosa, A.F., Shull, C.M., Brown, C.B., Alencar, S.A., Lents, C.A., Ellis, M. 2021. Effect of drying and/or warming piglets at birth under warm farrowing room temperatures on piglet rectal temperature over the first 24 h after birth. Translational Animal Science. txab060. <a href="https://doi.org/10.1093/tas/txab060">https://doi.org/10.1093/tas/txab060</a>.</li> <li>Kerns K, Sharif M, Zigo M, Miller D, Sutovsky S. 2020. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. Int J Mol Sci 21:2121.</li> <li>Sharif M, Kerns K, Sutovsky P, Bovin N, Miller. DJ  2021. Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner. Reproduction. 161:449-457.</li> <li>Johnson, JS, JM Maskal, AW Duttlinger, KR. Kpodo, BR McConn, CJ Byrd, BT Richert, JN Marchant-Forde, DC Lay, SD Perry, MC Lucy, TJ Safranski.   In utero heat stress alters the postnatal innate immune response of pigs. J. Anim. Sci. 98 (12):1-13.</li> <li>Bernhard, CJ, KG Sharp, TJ Safranski, WR Lamberson and MC Lucy.   Reproduction and reproductive tract morphology of male and female pigs whose mothers were heat stressed during the second month of gestation.  J. Anim Sci. 98(11): 1-12.</li> <li>Maskal, J.M., AW Duttlinger, KR Kpodo, BR McConn, CJ Byrd, JN Marchant-Forde, DC Lay, SD Perry, MC Lucy, TJ Safranski, JS Johnson.   Evaluation and mitigation of the effects of in utero heat stress on piglet growth performance, postabsorptive metabolism, and stress response following weaning and transport.  J. Anim. Sci., 98(9):1-13.</li> <li>Ciccarelli M, Giassetti MI, Miao D, Oatley MJ, Robbins C, Lopez-Biladeau B, Waqas MS, Tibary A, Whitelaw B, Lillico S, Park CH, Park KE, Telugu B, Fan Z, Liu Y, Regouski M, Polejaeva IA, Oatley JM. <a href="https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F32929012%2F&data=04%7C01%7C%7Cba0ab4d4dec04781120d08d918a68081%7Ce3fefdbef7e9401ba51a355e01b05a89%7C0%7C0%7C637567925660886506%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2FCO2ZQnjYR8%2BEY3cZtmmY4dvA9GeOB4yOHXI1mOtnJk%3D&reserved=0">Donor-derived spermatogenesis following stem cell transplantation in sterile NANOS2knockout males. </a>Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24195-24204.  PMID: 32929012</li> <li>Park CH, Jeoung YJ, Uh KJ, Park KE, Bridge J, Powell A, Li J, Pence L, Zhang Y, Liu T, Sun H, Gu Y, Shen Y, Wu J, Belmonte J-C, Telugu BP*. Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos. Stem Cell Reports. 2021 Jan 12;16(1):212-223. doi: 10.1016/j.stemcr.2020.11.011. PMID: 33338433</li> <li>Geisert R.D., A.E. Meyer, C.A. Pfeiffer, D.N. Johns, K. Lee, K.D. Wells, T.E. Spencer, and R.S. Prather. 2021. Gene Editing to Investigate Role of Conceptus Factors in Establishment of Pregnancy. Reproduction 161: R79-R88.</li> <li>Pfeiffer C.A., A.E. Meyer, K.E. Brooks, P.R. Chen, J. Milano-Foster, L.D. Spate, J.A. Benne, R. Cecil, M.S. Samuel, L.A. Ciernia, M.F. Smith, K.D. Wells, T.E. Spencer, R.S. Prather, and D. Geisert. 2020. Ablation of conceptus PTGS2 provides a new understanding of early pregnancy events in the pig. Biol Reprod<a href="https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.1093%2Fbiolre%2Fioz192&data=04%7C01%7C%7C8a2d946e44d24b4a54b708d916203c38%7Ce3fefdbef7e9401ba51a355e01b05a89%7C0%7C0%7C637565149969496019%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2BPGC6jMBNV2oISOZ13OzZ%2Bb04X4i%2FIFYBp9OT1cOgfs%3D&reserved=0"> 102:475-488. doi.org/10.1093/biolre/ioz192</a>.</li> <li>Cho J, Uh K, Ryu J, Fang X, Bang S, Lee K. Development of PCR based approach to detect potential mosaicism in porcine embryos. Journal of Animal Reproduction and Biotechnology. 2020 Dec. 35. 4.</li> <li>Uh K, Ryu J, Farrell K, Wax N, Lee K. TET family regulates the embryonic pluripotency of porcine preimplantation embryos by maintaining the DNA methylation level of NANOG. Epigenetics. 2020 Nov;15(11):1228-1242. doi: 10.1080/15592294.2020.1762392. Epub 2020 May 13.</li> <li>Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P (2020) Is the ubiquitin-proteasome system involved in the degradation of porcine beta-microseminoprotein during sperm capacitation? Int. J. Mol. Sci., 21(11):4151.</li> <li>Kerns K, Sharif M, Zigo M, Xu W, Hamilton L, Sutovsky M, Ellersieck M, Drobnis EZ, Oko R, Miller D, Sutovsky P (2020) Sperm cohort-specific zinc signature acquisition and capacitation-induced zinc flux regulate sperm-oviduct and sperm-zona pellucida interactions. Int. J. Mol. Sci., 21(6):2121; Special Issue on Advances in Molecular Regulation of Spermatozoa Function,</li> <li>Zigo M, Maňásková-Postlerová P, Zuidema D, Kerns K, Jonáková V, Tůmová L, Bubeníčková F, Sutovsky P (2020) Porcine model for the study of sperm capacitation, fertilization and male fertility. Cell Tissue Res., 380(2):237-262.</li> <li>Zuidema D, Sutovsky P (2020) The domestic pig as a model for the study of mitochondrial inheritance. Cell Tissue Res., 80(2):237-262. Special Issue on Animal Models,</li> <li>Pfeiffer, C.A., A.E. Meyer, K.E. Brooks, P.R. Chen, J.M. Foster, L.D. Spate, J.A. Benner, R. Cecil, M.S. Samuel, L.A. Ciernia, M.F. Smith, K.D. Wells, T.E. Spencer, R.S. Prather. 2020. Ablation of conceptus PTGS2 provides a new understanding of early pregnancy events in the pig. Biol. Reprod. Doi: 10/.1093/biolre/ioz192 PMID: 31616930, PMCID: PMC7523696.</li> <li>Stoian, A., R.R.R. Rowland, V. Petrovan, M. Sheahan, M.S. Samuel, K.M. Whitworth, K.D. Wells, J. Zhang, B. Beaton, M. Cigan, R.S. Prather. 2020. The use of cells from ANPEP knockout pigs to evaluate the role of aminopeptidase N (APN) as a receptor for porcine deltacoronavirus (PDCoV). Virology. 2020 Feb; 541:136-140. doi: 10.1016/j.virol.2019.12.007. Epub 2019 Dec 24. PMID: 32056711, PMCID: PMC7112016</li> <li>Chen, P.R., L.D. Spate, E.C. Leffeler, J.A. Benne, R.F. Cecil, T.K. Hord, R.S. Prather. 2020. Removal of hypotaurine from porcine embryo culture medium does not impair development of in vitro fertilization or somatic cell nuclear transfer-derived embryos at low oxygen tension. Molecular Reproduction and Development https:/doi: 10.1002/mrd.23393. PMID 32495478, PMCID: PMC7496716.</li> <li>Cecil, R.F., J.A. Benne, P.R. Chen, T.K. Hord, L.D. Spate, M.S. Samuel, R.S. Prather. 2020. Chemical stimulation of hypoxia in somatic cell nuclear transfer donor cells permits metabolic reprogramming and improved SCNT efficiency. Molecular Reproduction and Development https:/doi: 10.1002/mrd.23392 PMID 32558023, PMCID: PMC7496615.</li> <li>Lucas, C.G., A.M. Spate, M.S. Samuel, L.D. Spate, W.C. Warren, R.S. Prather, K.D. Wells. 2020. A novel swine sex-linked marker and its application across different mammalian species. Transgenic Research doi: 10.1007/s11248-020-00204-z. PMID: 32607872, PMCID: PMC7423754</li> <li>Ostedgaard, L.S., M.P. Price, K.M. Whitworth, M.H.A. Alaiwa, A.J. Fischer, A. Warrier, M. Samuel, L.D. Spate, P.D. Allen, B.M. Hilkin, G.S. R. Ibarra, M.E. Ortiz, B.J. Goodelll, S.E. Mather, L.S. Powers, M.R. Stroik, N.D. Gansmer, C.E. Hippee, K. Zarei, J.A. Goeken, T.R. Businga, E. A. Hoffman, D.K. Meyerholz, R.S. Prather, D.A. Stoltz, M.J. Welsh. 2020. Lack of submucosal glands impairs respiratory host defenses in pigs. eLife 2020;9:e59653. DOI: https://doi.org/10.7554/eLife.59653. PMID:33026343, PMCID: PMC7541087</li> <li>Koppes, E., B.K. Redel, M.A. Johnson, K.J. Shvorak, L. Ghaloul-Gonzalez, M.E. Yates, D.W. Lewis, S.M. Gollin, Y. Wu, S.E. Christ, M. Yerle, A. Leshinski, L.D. Spate, J. Benne, S. Murphy, M.S. Samuel, E.M. Walters, S.A. Hansen, K.D. Wells, J. Vockley, R.S. Prather, R.D. Nicholls. 2020. A porcine model of phenylketonuria generated by CRISPR/Cas9 genome editing. JCI Insight doi: 10.1172/jci.insight.141523. PMID: 33055427 PMCID: PMC7605535.</li> </ol>   Boar Performance <ol> <li>Mills, K.M., Aryal, U.K., Sobreira, T., Minton, A.M., Casey, T., and K.R. Stewart. 2020. Shotgun proteome analysis of seminal plasma differentiate boars by reproductive performance.  Theriogenology 157: 130-139.  <a href="https://doi.org/10.1016/j.theriogenology.2020.07.013">doi.org/10.1016/j.theriogenology.2020.07.013</a></li> <li>Lents, C.A., Supakorn, C., DeDecker, A.E., Phillips, C.E., Boyd, R.D., Vallet, J.L., Rohrer, G.A., Foxcroft, G.R., Flowers, W.L., Trottier, N.L., Salak-Johnson, J.L., Bartol, F.F., Stalder, K.J. 2020. Dietary lysine-to-energy ratios for managing growth and pubertal development in replacement gilts. Applied Animal Science. 36(5):701-714. <a href="https://doi.org/10.15232/aas.2020-02016">https://doi.org/10.15232/aas.2020-02016</a>. DOI: <a href="https://doi.org/10.15232/aas.2020-02016">https://doi.org/10.15232/aas.2020-02016</a></li> <li>Rempel, L.A., Parrish, J.J., Miles, J.R. 2020. Genes associated with chromatin modification within the swine placenta are differentially expressed due to factors associated with season. Frontiers in Genetics. 11:1019. <a href="https://doi.org/10.3389/fgene.2020.01019">https://doi.org/10.3389/fgene.2020.01019</a>.</li> <li>Belstra, B.A., Willenburg, K.L., Gómez-López, D.H., Knox, R.V., Stewart, K.R., 2020. Effects of the number of sperm and site of uterine semen deposition on conception rate and the number of embryos in weaned sows receiving a single fixed-time insemination. Journal of Animal Science 98.</li> <li>Kerns K, Jankovitz J, Minton A, Kuster C, Robinson J, Sutovsky P (2020) Relationship between the length of sperm tail mitochondrial sheath and fertility traits in boars used for artificial insemination. Antioxidants, 9(11):1033. doi: 10.3390/antiox9111033</li> <li>Parrish JJ, PREDICITION OF FERTILITY IN MALES, US Patent App. 16/024,335; Patent granted May 2020.</li> </ol>   Genetics and Genomics <ol> <li>Johnson, J.S., Stewart, K.R., Safranski, T.J., Ross, J.W. and L.H. Baumgard.  2020.  In utero heat stress alters postnatal phenotypes in swine.  Theriogenology, 154:110-119. <a href="https://doi.org/10.1016/j.theriogenology.2020.05.013">doi.org/10.1016/j.theriogenology.2020.05.013</a></li> <li>Wijesena, H.R., SD Kachman, CA Lents, JJ Riethoven, MD Trenhaile-Grannemann, TJ Safranski, ML Spangler, DC Ciobanu. Fine mapping genetic variants associated with age at puberty and sow fertility using SowPro90 genotyping array.   J. Anim. Sci. 98(10): 1-12.</li> <li>Johnson, J.S., KR Stewart, TJ Safranski, JW Ross, LH Baumgard. In utero heat stress alters postnatal phenotypes in swine. 2020. Theriogenology, 154: 110-119</li> </ol>   Sow and Gilt Performance <ol> <li>Belstra, B.A., Willenburg, K.L, Gómez-López, D.H., Knox, R.V., and K.R. Stewart. 2020. Effects of number of sperm and site of uterine deposition on conception rate and number of embryos in weaned sows receiving a single fixed time insemination.  J Anim Sci.,98:9, 1-10. doi:10.1093/jas/skaa260</li> <li>Suarez-Trujillo, A., Senn, K., Teeple, K., Casey, T., and K.R. Stewart. 2020. Technical Note: A standardized model to study effects of varying 24 h colostrum dose on postnatal growth and development. TAS, txaa212, <a href="https://doi.org/10.1093/tas/txaa212">doi.org/10.1093/tas/txaa212</a>.</li> <li>Leonard, S.M., Xin, H., Brown-Brandl, T.M., Ramirez, B.C., Johnson, A.K., Dutta, S., Rohrer, G.A. 2021. Effects of farrowing stall layout and number of heat lamps on sow and piglet behavior. Applied Animal Behaviour Science. 239:105334. <a href="https://doi.org/10.1016/j.applanim.2021.105334">https://doi.org/10.1016/j.applanim.2021.105334</a>.</li> </ol>   Teaching and extension <ol> <li>Mills, K.M., Schinckel, A.P., Casey, T.M., and K.R. Stewart. 2020. Evaluation of on-farm indicators of gilt reproductive performance potential at 21 days of age.  TAS, txaa210, <a href="https://doi.org/10.1093/tas/txaa210">doi.org/10.1093/tas/txaa210</a>.  </li> </ol>

Impact Statements

Seasonal alterations to chromatin modifiers within the placenta are impacted by breeding season and season of semen collection.

Date of Annual Report: 07/21/2022

Report Information

Annual Meeting Dates: 05/24/2022 - 05/25/2022
Period the Report Covers: 10/01/2021 - 09/30/2022

Participants

John Parrish, University of Wisconsin-Madison (Chair); Jeremy Miles (Secretary), USDA-USMARC; Kara Stewart (Local Host, chair of Biannual Symposium), Purdue University; Brett White, University of Nebraska-Lincoln; Tim Safranski, University of Missouri; Kayla Mills, USDA-BARC; Lea Rempel, USDA-USMARC; Jason Ross, Iowa State University (second day); Visitor - Wayne Singleton

Brief Summary of Minutes

May 24, 2022: The meeting was called to order at 9:00 am by John Parrish (Chair) and Kara Stewart (Local Host). Kara introduced Kayla Mills, her past PhD student, who would be serving as a new member to NCEA57 from USDA-BARC. There was some discussion on the historical aspects of the committee by various members with John Parrish highlighting the historical document located on the NIMSS system. John Parrish also discussed some details on submitting the final report and to get access to the NIMSS system via Christian Hamilton. It was also discussed that we need to officially add Kayla Mills as a representative and work with Christian Hamilton on this process.

Station reports started at 9:15 am: 1) USDA-USMARC, Nebraska, Lea Rempel; 2) Purdue University, Indiana, Kara Stewart; 3) USDA-BARC, Maryland, Kayla Mills. Following these morning station reports, John Parrish nominated Kayla Mills for the secretary for next year’s annual meeting, seconded by Jeremy Miles, and approved by all representative presence. There was discussion about adding Karl Kerns from Iowa State University and someone from NC State University. John Parrish also mentioned his willingness to host the 2023 NCERA57 meeting and highlighted that it could be his last meeting. At 11:00 am Mark Mirando, USDA-NIFA National Science Liaison, joined via Zoom and introduced Kamilah Grant, the new USDA-NIFA Animal Reproduction NPL. Mark then provided the USDA-NIFA update. After concluding the USDA-NIFA update, John Parrish initiated a discussion about predatory journals and publications, following this discussion the Committee had lunch with producers, speakers, and attendees of the NCERA57 Biannual Symposium.

At 12:30 pm, the Committee reconvened with Kara Stewart (Symposium Organizing Chair) kicking off the 2022 NCERA57 Biannual Symposium: Managing the Hyperprolific Sow. This symposium was originally scheduled for 2020, but due to the COVID pandemic had been postponed 2 years. The speaker and their presentation titles are as followed:

Changes in the physiology and re-thinking management of the modern sow – Dr. Nicoleme Soede

An overview of sow performance-challenges we are facing today – Mr. Ron Ketchem

Practical approaches to feeding the modern sow – Dr. Laura Greiner

What’s happening between sow and her litter? – Dr. Walt Hurley

Optimizing litter performance pre-weaning and beyond – Dr. Greg Krahn

Dietary management of the transition animal: Perspective from dairy cows to sows – Dr. Bob Vansaun

The Symposium concluded at 5:00 pm and the Committee adjourned for the day.

May 25, 2022: Prior to the Committee reconvening at 7:45 am, Wayne Singleton setup a display of historical reproduction equipment used for animal research and production. The meeting was called to ordered by John Parrish and the other station reports were given: 1) University of Wisconsin, Wisconsin, John Parrish; 2) University of Nebraska – Lincoln, Nebraska, Brett White; 3) Iowa State University, Iowa, Jason Ross. Following the station reports, there was a brief business meeting where the committee confirmed Kayla Mill as next year’s secretary and set the 2023 meeting date for May 23-24, 2023 at the University of Wisconsin hosted by John Parrish. In addition, further discussion about inviting Karl Kerns was provided and noted that Kayla Mills and any new participants would need to get appropriate forms filled out under the participant tab on the NIMSS website. Jason Ross also noted the Iowa State University might be a good option for host the next symposium in 2024. Finally, the Committee thanked Kara Stewart for all her efforts in organizing the Symposium and all the speakers and sponsors (United Animal Health, Merk, AASV, Summit Smart Farms, Topigs Norsvin, CSA Animal Nutrition). The Committee adjourned at 10:00 am and Kara Stewart provided the group a tour of the Creighton Hall Animal Science building at Purdue University.

Accomplishments

<ol> <li>In support of Objective 1 & 3 – Reported the influence of fish oil supplementation on boar sperm quantity and spermatogenesis (Wisconsin, Iowa, and Ohio).</li> <li>In support of Objective 1 & 3– Published a book chapter on Animal Andrology (Illinois and Indiana).</li> <li>In support of Objectives 1, 3, & 4 – Identified sperm proteome following acute scrotal temperature in pigs (Missouri and Illinois).</li> <li>In support of Objective 1, 3, & 4 – Identified metabolites involved in binding of sperm to oviduct glycans to extend sperm lifespan (Missouri and Illinois).</li> <li>In support of Objective 1, 3, 4 & 8 – Continued collaboration on USDA-NIFA grant: Proteomic identification of molecular pathways and biomarker related to porcine seasonal heat-induced infertility in males (Illinois and Wisconsin).</li> <li>In support of Objective 1, 3, 4 & 8 – Continued collaboration on NIH grant: Accumulation, storage, and release of sperm in the oviduct (Illinois and Missouri).</li> <li>In support of Objective 2, 3 & 4 – Characterized kisspeptin, NKB, and NK3R in the hypothalamus of gilts following Altrenogest treatment (USMARC, West Virginia, Ohio, and Mississippi).</li> <li>In support of Objective 2, 3 & 4 – Identified biomarkers of fertility in gilts (BARC & Indiana)</li> <li>In support of Objective 2, 3, 4 & 8 – Continued collaboration on National Pork Board Grant: Investigating survivability in swine (BARC, Indiana, and Iowa)  </li> <li>In support of Objective 3 & 4 – Characterized extensive transcriptomic differences in spherical, ovoid and tubular conceptus during the initiation of elongation in gilts (USMARC and Nebraska).</li> <li>In support of Objective 3, 4 & 8 – New USDA-NIFA grant awarded: Influence of maternal and embryonic extracellular vesicles on the initiation of porcine conceptus elongation, #2021-67015-34416 (Nebraska and USMARC).</li> <li>In support of Objective 5 – Planning committee for the SowBridge program, an extension program targeted to translating science into usable information for swine farms (Indiana, Missouri, and Illinois).</li> <li>In support of Objective 5 – Organized and presented an Extension Webinar on farrowing induction in sows (Indiana and Pennsylvania).</li> <li>In support of Objective 5 & 7 – Organized and hosted the 2022 NCERA57 Biannual Symposium: Managing the Hyperprolific Sow (Indiana, Wisconsin, Missouri).</li> </ol>

Publications

Basic Science and Technology <ol> <li>Cline, P.M., Tsai, T.C., Lents, C.A., Stelzleni, A.M., Dove, C.R., Azain, M. 2021. Interaction of dietary carbohydrate and fat on glucose metabolism in growing pigs. Domestic Animal Endocrinology. 78. Article 106655. <a href="https://doi.org/10.1016/j.domaniend.2021.106655">https://doi.org/10.1016/j.domaniend.2021.106655</a>.</li> <li>Meinen, R.J., Spiegal, S.A., Kleinman, P.J., Flynn, K.C., Goslee, S.C., Mikesell, R.E., Church, C., Bryant, R.B., Boggess, M.V. 2022. Opportunities to implement manureshed management in the Iowa, North Carolina, and Pennsylvania swine industry. Journal of Environmental Quality. <a href="https://doi.org/10.1002/jeq2.20340">https://doi.org/10.1002/jeq2.20340</a>.</li> <li>Velez, F.J., Bosilevac, J.M., Singh, P. 2021. Validation of high-resolution melting assays for the detection of virulent strains of Escherichia coli O26 and O111 in beef and pork enrichment broths. Food Control. 128. Article 108123. <a href="https://doi.org/10.1016/j.foodcont.2021.108123">https://doi.org/10.1016/j.foodcont.2021.108123</a>.</li> </ol>  Boar Performance <ol> <li>Andrade, A., Grossfeld, R., Knox, R. V. 2021 In vitro effects of commercial freezing and thawing extenders on boar sperm quality. Anim. Reprod. Sci. Pages 106906 DOI: <a href="https://doi.org/10.1016/j.anireprosci.2021.106906">https://doi.org/10.1016/j.anireprosci.2021.106906</a>.</li> <li>Sharif, M., Hickl, V., Juarez, G., Di, X., Kerns, K., Sutovsky, P., Bovin, N., Miller, D. J.   Hyperactivation is sufficient to release sperm from immobilized oviduct glycans.  Sci Rep 12, 6446. <a href="https://doi.org/10.1038/s41598-022-10390-x">https://doi.org/10.1038/s41598-022-10390-x</a></li> <li>Sharif, M., Kerns, K., Sutovsky, P., Bovin, N., Miller, D.J.   Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner.  Reproduction. 161(4):449-457. doi: 10.1530/REP-20-0474.</li> </ol>  Sow and Gilt Performance <ol> <li>Arend, L.S., Knox, R.V. 2021. Fertility responses of melatonin-treated gilts before and during the follicular and early luteal phases when there are different temperatures and lighting conditions in the housing area. Anim Reprod Sci, 230, 106769. doi:10.1016/j.anireprosci.2021.106769</li> <li>Knox, R.V., Arend, L.S., Buerkley, A.L., Patterson J.L., Foxcroft, G.R. 2021. Effect of physical or fenceline boar exposure and gonadotropins on fertility in gilts. J. Anim. Sci. DOI: 10.1093/jas/skab348.</li> <li>Lindo, A.N., Thorson, J., Bedenbaugh, M.N., Mccosh, R.B., Lopez, J.A., Young, S.A., Meadows, L.J., Bowdridge, E.C., Fergani, C., Freking, B.A., Lehman, M.N., Hileman, S.M., Lents, C.A. 2021. Localization of kisspeptin, NKB, and NK3R in the hypothalamus of gilts treated with the progestin altrenogest. Biology of Reproduction. Article 103. <a href="https://doi.org/10.1093/biolre/ioab103">https://doi.org/10.1093/biolre/ioab103</a>.</li> <li>Miles, J.R., Walsh S.C., Rempel L.A., Pannier A.K.. 2022. Mechanisms regulating the initiation of porcine conceptus elongation.  Mol Reprod Dev.   DOI: 10.1002/mrd.23623.</li> <li>Mills, K.M., Ferreira, C.R., Stewart, K.R., Casey T.M.   Biomarkers predictive of long-term fertility found in vaginal lipidome of gilts at weaning.  J Anim Sci DOI: <a href="https://doi.org/10.21203/rs.3.rs-210669/v1">https://doi.org/10.21203/rs.3.rs-210669/v1</a></li> <li>Mills, K.M., Shirley, L.K., Sharp, K., Garcia, R., Suarez-Trujillo, A., Stewart, K.R. 2021. Effects of induction on the farrowing process and piglet blood parameters at time of birth. TAS 5:108.  doi: 10.1093/tas/txab032 </li> <li>Vande Pol, K.D., Tolosa, A.F., Bautista, R.O., Willard, N.C., Gates, R.S., Shull, C.M., Brown, C.B., Alencar, S.A., Lents, C.A., Ellis, M. 2021. Effects of drying and providing supplemental oxygen to piglets at birth on rectal temperature over the first 24 h after birth. Translational Animal Science. 5(3). Article txab095. <a href="https://doi.org/10.1093/tas/txab095">https://doi.org/10.1093/tas/txab095</a>.</li> <li>Walsh, S.C., Miles, J.R., Keel-Mercer, B.N., Rempel, L.A., Wright-Johnson, E.C., Lindholm-Perry, A.K., Oliver, W.T., Pannier, A.K. 2022. Global analysis of differential gene expression within the porcine conceptus transcriptome as it transitions through spherical, ovoid, and tubular morphologies during the initiation of elongation. Molecular Reproduction and Development. Article 23553. <a href="https://doi.org/10.1002/mrd.23553">https://doi.org/10.1002/mrd.23553</a></li> </ol>  Genetics and Genomics <ol> <li>Herrera-Uribe, J., Wiarda, J., Sivasankaran, S.K., Daharsh, L., Liu, H., Byrne, K.A., Smith, T.P., Lunney, J.K., Loving, C.L., Tuggle, C.K. 2021. Reference Transcriptomes of Porcine Peripheral Immune Cells Created Through Bulk and Single-Cell RNA Sequencing. Frontiers in Genetics. 12. Article 689406. <a href="https://doi.org/10.3389/fgene.2021.689406">https://doi.org/10.3389/fgene.2021.689406</a>.</li> </ol> Teaching and Extension <ol> <li>Hines, E., Romoser, M., Cover, J. Improving the gilt selection process.  Penn State Extension Report.  <a href="https://extension.psu.edu/improving-the-gilt-selection-process">https://extension.psu.edu/improving-the-gilt-selection-process</a></li> <li>Knox, R. 2021. The New Building Designs on Swine Farms in China. June 2021. Illinois AgriNews.</li> <li>Knox, R. 2021.  New technology used in the swine breeding barn. Dec. 2021. Illinois AgriNews.</li> </ol>  Abstracts/Posters/Book Chapter/Professional Presentations <ol> <li>Arend, L.S., Vinas, R.F., Silva, G., Lower, A., Connor, J.F., Knox. R.V.  Effects of nursing a large litter and ovarian response to gonadotropins at weaning on subsequent fertility in first parity sows. Journal of Animal Science, Volume 100, Issue Supplement_2, May 2022, Page 85, <a href="https://doi.org/10.1093/jas/skac064.137">https://doi.org/10.1093/jas/skac064.137</a></li> <li>Hauxwell, K., Ostrand, L.M., Schmidt, T.B., Psota, E., Mote, B.E., Rohrer, G.A. 2021. Activity and bone lesion analysis on gilt retention to the breeding herd [abstract]. Journal of Animal Science. 99(Supplement 1):154. <a href="https://doi.org/10.1093/jas/skab054.264">https://doi.org/10.1093/jas/skab054.264</a>.</li> <li>Hines E., Stewart, K.R.   Farrowing induction and your sows.  Extension Webinar.  Penn State Extension.  <a href="https://extension.psu.edu/farrowing-induction-and-your-sows-webinar">https://extension.psu.edu/farrowing-induction-and-your-sows-webinar</a></li> <li>Knox, R. V. 2021. Overview of Management of Reproduction: Pigs. Merck Veterinary Manual. pp 1-22. Management of Reproduction: Pigs - Merck Veterinary Manual (merckvetmanual.com)</li> <li>Knox, R.V. 2021. Gilt puberty, subsequent fertility, and incidence of silent heat in response to boar exposure and hormone induction, A.D. Leman Pre-Conference Workshop.</li> <li>Knox, R.V. 2022. Tools used and needed in the gilt pool. Journal of Animal Science, Volume 100, Issue Supplement_2, May 2022, Pages 9–10, <a href="https://doi.org/10.1093/jas/skac064.013">https://doi.org/10.1093/jas/skac064.013</a></li> <li>Ostrand, L.M., Tranhaile-Grannemann, M.D., See, G., Schmidt, T.B., Psota, E., Rohrer, G.A., Mote, B.E. 2021. Utilization of NUtrack to assess variance components and heritability of activity traits [abstract]. Journal of Animal Science. 99(Supplement 1):17. <a href="https://doi.org/10.1093/jas/skab054.030">https://doi.org/10.1093/jas/skab054.030</a>.</li> <li>Parrish, J.J., Gall, T., Wilson, M., Ballard, M. 2022. Salmate fish oil supplement to boars and impact on sperm production and spermatogenesis.</li> <li>Stewart, K.R., Knox, R.V. 2022. Applied animal andrology: Boar in Manual of Animal Andrology, edited by P.J. Chenoweth and S.P. Lorton, CAB International, 2022, pp95-107.</li> <li>Studer, J.M., Kiefer, Z.E., Gianluppi, R., Adur, M.K., Shultz, R.B., Horst, E.A., Mayorga, E.J., Keating, A.F., Baumgard, L.H., Lents, C.A., Ross, J.W. 2021. Direct and indirect effects of heat stress on the hepatic and ovarian proteome in gilts [abstract]. Journal of Animal Science. 99(Supplement 1):106-107. <a href="https://doi.org/10.1093/jas/skab054.173">https://doi.org/10.1093/jas/skab054.173</a>.</li> <li>Vinas, R.F., Arend, L.S., Silva, G., Lower, A.J., Connor, J.F., Kaustubh, B., Furugen, A., Andrade, A., Knox. R.V. Evaluation of electrical impedance spectroscopy to determine swine herd reproductive status using the accuracy of ultrasound and hormonal assays as diagnostic tools. Journal of Animal Science, Volume 100, Issue Supplement_2, May 2022, Page 84, <a href="https://doi.org/10.1093/jas/skac064.135">https://doi.org/10.1093/jas/skac064.135</a></li> </ol>

Impact Statements

Hosted the 2022 NCERA57 Biannual Symposium: Managing the Hyperprolific Sow

Date of Annual Report: 07/21/2023

Report Information

Annual Meeting Dates: 05/23/2023 - 05/24/2023
Period the Report Covers: 10/01/2022 - 09/30/2023

Participants

Amy Desaulniers, Nebraska - University of Nebraska (new member); Jean Feugang, Mississippi - Mississippi State University; Kamilah Grant, USDA-NIFA Representative (afternoon session); Rodney Geisert, Missouri - University of Missouri; Elizabeth Hines, Pennsylvania - Penn State University; Jay Johnson, Indiana - USDA-ARS; Karl Kerns, Iowa - Iowa State University; Robert Knox, Illinois - University of Illinois; Jeremy Miles (Chair/Secretary), Nebraska - USDA-ARS; David Miller, Illinois - University of Illinois; Kayla Mills, Maryland - USDA-ARS; Mark Mirando, USDA-NIFA Representative (afternoon session); Benny Mote, Nebraska - University of Nebraska; John Parrish, Wisconsin - University of Wisconsin; Lea Rempel, Nebraska - USDA-ARS; Jason Ross, (Administrative Advisor), Iowa - Iowa State University; Timothy Safranski, Missouri - University of Missouri.

Brief Summary of Minutes

Brief Summary of Minutes for the Annual Meeting:

May 23, 2023: The virtual meeting hosted on Teams was called to order by Jeremy Miles (Chair) at 9:00 am. After a short welcome for the committee members, an apology was given by the Chair for having to move the meet to a virtual format due to the limited number of members that could make an in-person meeting (only 4 total could make it). A brief discussion was given that the lack of travel capacity was likely due to many of the members traveling to Europe within two weeks for the International Conference on Pig Reproduction (ICPR), which had been postponed since 2020 because the COVID pandemic. Several of the committee members were presenting and serving as organizers/chairs at the ICPR meeting, which provide good international exposure for the research for many of the committee members. Thanks was also given to John Parrish for originally offering to host the meeting at the University of Wisconsin in Madison. Following this discussion (~9:10 am), brief station reports were given for Pennsylvania - Penn State University, Elizabeth Hines; Wisconsin - University of Wisconsin, John Parrish; and Illinois - University of Illinois, David Miller.

At 10:30 am, the business meeting began to discuss details regarding the Biannual symposium (scheduled for 2024), five-year project rewrite (due in 2024), new members, potential collaborations, and other details. First, the committee discussed potential topics for the Biannual symposium, which included effects of viruses/diseases on swine reproduction, tools for fixed timed single-inseminations, and prolapse issues. The committee came to a consensus on the overall topic factoring on external factors that influence reproductive (viruses, disease, seasonality, etc). An initial title, “Maximizing Efficiency of Swine Herds”, was suggested and could encompass all these external topics. Tim Safranski, Elizabeth Hines, and Lea Rempel volunteered to serve on the organizing committee for the Biannual symposium. The location and date for the next annual meeting/Biannual symposium was then discussed. Brett White suggested and volunteered to have the University of Nebraska Lincoln host the meeting and symposium, which was agreed by the committee. A brief discussion was made about moving the meeting from the usual date (Tuesday a week prior to Memorial Day) given the low ability of members to travel for the current meeting, but it was decided that usual date was still the best and it was agreed to have the meeting on May 21^st and 22^nd, with the symposium to be in held the afternoon of May 21^st after a lunch for the speakers and attendees. Brett also volunteered to serve as secretary with Kayla Mills moving to the chair position for 2024. It was also noted to send any recommendations for speakers to the organizing committee. Next, the Chair highlighted that the 5-year NCERA57 rewrite was coming up and would be due next FY with current project expiring September 30, 2024. The renewal process instructions are highlighted on the NIMSS website (https://www.ncra-saes.org/new-renewal-nc-project-instructions) and the process needs to be requested and initiated by 09/15/23. Jeremy Miles volunteered to lead the process with Dave Miller and Kayla Mills volunteering to help with the rewrite process. John Parrish and Rod Geisert agreed to provide advisory assistance as past members going through this process. There was a brief discussion about potential new members for the committee. Several names were mentioned including Amy Desaulniers (University of Nebraska Lincoln; guest at this meeting and has officially joined the committee), Mark Knauer (NC State University; has already been invited by Jeremy Miles and Elizabeth Hines and plans to join in FY24), Jeff Wiegert and Greg Johnson (Texas A&M), Clay Lents (USDA-USMARC, has been invited), and some industry representatives (Steven Terlouw, DNA Genetics/Pillen Farms; and Ashley DeDecker, Smithfield). Finally, new potential collaborations were discussed and these included studies of micronutrients on swine production and means of implement teaching components with students and producers as well. This led to a discussion of potential websites for teaching and extension components. John Parrish highlighted the Animal Science Image Gallery (https://www.animalimagegallery.org/) would be a great option for uploading teaching and extension components for distribution of a variety of images and files. The business meeting concluded at ~ 12:00 pm and the committee suspended the meeting for lunch.

At 1:00 pm, the committee reconvened with brief station reports for Nebraska - University of Nebraska, Brett White; and Nebraska - USDA, Lea Rempel. At 2:00 pm, Kamilah Grant and Mark Mirando joined the meeting and provided an update on USDA-NIFA grants and information about specific programs. Following the NIFA update, brief station reports were given for Nebraska - USDA, Jeremy Miles; and Mississippi - Mississippi State University, Jean Feugang. To close out the meeting, Rod Geisert requested that condolence be noted in the minutes for the recent loss of Dr. Billy Day on April 22, 2023. Dr. Day was one of the founding members of the NCERA57 committee and the committee is grateful for all the contributions that Dr. Day provided this committee and Swine reproductive knowledge. The meeting was adjourned at 4:00 pm by the Chair and virtual host.

Respectively submitted,

Jeremy Miles – 2023 Chair/Secretary NCERA57 Swine Reproductive Physiology

Accomplishments

For FY2023 reporting period, the NCERA57 committee yielded 33 multi-station publications including peer-review journal articles and abstracts associated with swine reproduction, physiology, and genetics of which contributed to the committee’s Objectives highlighted in the Impact bullets below.  In addition, five committee members presented invited presentation at the 11th International Conference on Pig Reproduction in Ghent, Belgium providing international exposure of the current research being performed from members of the committee.  The committee also continues to multi-station collaborations on extramural grants (one NIH and three USDA-AFRI-NIFA grants active grants), two of these grants are new during this reporting period.  Finally, the committee initiated the planning for the NCERA57 Biannual Symposium with the proposed title “Maximizing Efficiency of Swine Herds” that will focus on external factors that influence reproductive productivity.  NCERA57 Project Objectives: <ol> <li>Improve boar performance through research on: technology to reduce the number of sperm required for maximal fertility in artificial insemination, impacts of seasonal infertility on spermatogenesis, semen and male fertility as well as methods to mitigate these impacts, improved quality of insemination doses of semen through improved semen evaluation, additives and male selection, and evaluation of timed AI procedures with frozen semen.</li> <li>Improved sow and gilt performance via research on: endocrine control of female reproduction, development of protocols for timing of ovulation and insemination, pubertal development, mammary gland development and physiology, epigenetic impacts on the fetus of summer heat stress and the potential effects of endocrine disruptors.</li> <li>Increase the basic knowledge of folliculogenesis, spermatogenesis, fertilization, early embryo development, conceptus signaling for the establishment of pregnancy, uterine morphogenesis, endometrial secretion and immune function, and regulation of placental attachment and growth to be applied to future methods for improving reproduction efficiency of swine.</li> <li>Increase the utilization of the rapidly advancing technology of functional genomics, proteomics and bioinformatics toward research regarding many of the items in objectives 1, 2 and 3.</li> <li>Provide unique mechanisms for open scientific exchange and dialogue to advance research initiatives of participating scientists, and exchange of information and techniques that enhance teaching and adaptation of technology through the cooperative extension service, higher education and the swine industry.</li> <li>Unique to this committee is the development of innovative teaching methods for undergraduate education. This will be continued with emphasis on how to increase student engagement, development of on-line resources that are available to multiple universities and instructors, and how to adapt to the changing type of student present in the land-grant universities.</li> <li>Continuation of the biennial symposia that brings together university educators, extension scientists, reproductive physiologists and swine industry representatives to ensure researchers understand the swine industry, the industry is aware of current developments and technologies and educators can gather the latest information to implement in university and extension programs.</li> <li>Collaborative USDA research grants between groups of researchers will be pursued to fund research on the objectives.</li> </ol> Impacts Statement for the Committee are illustrated by contributions of multi-station locations to the NCEAR57 Project Objectives as followed:

Publications

Publications: Basis Science and Technology <ol> <li>Li, Y., Adur, M.K., Lonergan, S.M., Keating, A.F., Ross, J.W.   MicroRNA21 inhibition affects porcine oocyte maturation and alters protein expression critical for metabolic pathway function.  Mol Reprod Dev.  89(10):443-458. <a href="https://doi:10.1002">https://doi:10.1002/mrd.23641</a>.</li> <li>Miller, K.A., Johnson, E.M., Matchan, S.A., Goehring, D., Ross, J.W., Gabler, N.K.   Strategies to manage barn feed supply to prolong and hold late finishing pigs during a supply chain disruption.  Transl Anim Sci. 7(1):txac166. <a href="https://doi:10.1093">https://doi:10.1093/tas/txac166</a>.</li> <li>Studer, J.M., Schweer, W.P., Gabler, N.K., Ross, J.W.   Functions of manganese in reproduction. Anim Reprod Sci. 238:106924. <a href="https://doi:10.1016">https://doi:10.1016/j.anireprosci.2022.106924</a>.</li> </ol>  Boar Performance <ol> <li>de Andrade, A.F.C., Balogun, K., Machaty, Z., Knox, R.V.  Effects of supplemental antioxidants on in vitro fertility measures for cryopreserved boar spermatozoa. Theriogenology. 200:33-42. <a href="https://doi:10.1016">https://doi:10.1016/j.theriogenology</a>.</li> <li>Andrade, A.F.C., Knox, R.V., Torres, M.A., Pavaneli, A.P.P.   What is the relevance of seminal plasma from a functional and preservation perspective? Anim Reprod Sci. 246:106946. <a href="https://doi:10.1016">https://doi:10.1016/j.anireprosci.2022.106946</a>.   </li> <li>Lawlor, M., Zigo, M., Kerns, K., Cho, I.K., Easley, C.A., Sutovsky, P.  Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease. Intl J of Molc Sci. 23(13):7163.  <a href="https://doi:10.3390">https://doi:10.3390/ijms23137163</a>.</li> <li>Nogueira, E., Tirpák, F., Hamilton, L., Zigo, M., Kerns, K., Sutovsky, M., Kim, J., Volkmann, D., Jovine, L., Taylor, J., Schnabel, R., Sutovsky, P.  A Non-Synonymous Point Mutation in a WD-40 Domain Repeat of EML5 Leads to Decreased Bovine Sperm Quality and Fertility. Front. Cell Dev. Biol.  <a href="https://doi:10.3389">https://doi:10.3389/fcell.2022.872740</a>.</li> <li>Sharif, M., Hickl, V., Juarez, G., Di, X., Kerns, K., Sutovsky, P., Bovin, N., Miller, D.J.   Hyperactivation is sufficient to release sperm from immobilized oviduct glycans.  Sci Rep.  12:6446. <a href="https://doi.org/10.1038/s41598-022-10390-x">https://doi.org/10.1038/s41598-022-10390-x</a>.</li> <li>Spooner-Harris, M., Kerns, K., Zigo, M., Sutovsky, P., Balboula, A., Patterson, A. A re‑appraisal of mesenchymal‑epithelial transition (MET) in endometrial epithelial remodeling. Cell Tissue Res. 391(2):393-408. <a href="https://doi:10.1007">https://doi:10.1007/s00441-022-03711-z</a>.</li> <li>Zigo, M., Kerns, K., Sen, S., Essien, C., Oko, R., Xu, D., Sutovsky, P.  Zinc is a master-regulator of sperm function associated with binding, motility, and metabolic modulation during porcine sperm capacitation. Commun. Biol. 5(1):538.  <a href="https://10.1038/s42003-022-03485-8">https://10.1038/s42003-022-03485-8</a>.</li> </ol>  Sow and Gilt Performance <ol> <li>Arend, L.S., Vinas, R.F., Silva, G.S., Lower, A.J., Connor JF, Knox RV.   Effects of nursing a large litter and ovarian response to gonadotropins at weaning on subsequent fertility in first parity sows. J Anim Sci. 101:skac398. <a href="https://doi:10.1093">https://doi:10.1093/jas/skac398</a>.</li> <li>Bhatia, V., Stevens, T., Derks, M.F.L., Dunkelberger, J., Knol, E.F., Ross, J.W., Dekkers, J.C.M.   Identification of the genetic basis of sow pelvic organ prolapse.  Front Genet.  14:1154713.  <a href="https://doi:10.3389">https://doi:10.3389/fgene.2023.1154713</a>.</li> <li>Walsh, S.C., Miles, J.R., Broeckling, C.D., Rempel, L.A., Wright-Johnson, E.C., Pannier, A.K.  Secreted metabolome of porcine blastocysts encapsulated within in vitro 3D alginate hydrogel culture systems undergoing morphological changes provides insights into specific mechanisms involved in the initiation of porcine conceptus elongation. Reprod Fertil Dev. 35(5):375-394. <a href="https://doi.org/10.1071/RD22210">https://doi.org/10.1071/RD22210</a>.</li> </ol>  Seasonality and Heat Stress <ol> <li>Adur, M.K., Seibert, J.T., Romoser, M.R., Bidne, K.L., Baumgard, L.H., Keating, A.F., Ross, J.W.   Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period.  J Anim Sci. 100(7):skac129. <a href="https://doi:10.1093">https://doi:10.1093/jas/skac129</a>.</li> <li>Brown-Brandl, T.M., Hayes, M.D., Rohrer, G.A., Eigenberg, R.A.  Thermal comfort evaluation of three genetic lines of nursery pigs using thermal images. Biosystems Engineering. 225:1-12. <a href="https://doi.org/10.1016/j.biosystemseng.2022.11.002">https://doi.org/10.1016/j.biosystemseng.2022.11.002</a>.</li> <li>Raber, V.L., Pritchett, R.K., Robbins, L.A., Stewart, K.R., Gaskill, B.N., Green-Miller, A.R., Johnson, J.S.  Evaluating the temperature preferences of sexually mature Duroc, Landrace, and Yorkshire boars.  Transl Anim Sci.  May 29;7(1):txad060. <a href="https://doi:10.1093">https://doi:10.1093/tas/txad060</a>.</li> <li>Ramirez, B.C., Hoff, S.J., Hayes, M.D., Brown-Brandl, T.M., Harmon, J.D., Rohrer, G.A. 2022.  A review of swine heat production: 2003 to 2020. Frontiers in Animal Science. 3. Article 908434. <a href="https://doi.org/10.3389/fanim.2022.908434">https://doi.org/10.3389/fanim.2022.908434</a>.</li> <li>Roach, C.M., Bidne, K.L., Romoser, M.R., Ross, J.W., Baumgard, L.H., Keating, A.F.  Impact of heat stress on prolactin-mediated ovarian JAK-STAT signaling in postpubertal gilts.  J Anim Sci. 100(7):skac129. <a href="https://doi:10.1093">https://doi:10.1093/jas/skac129</a>.</li> <li>Romoser, M.R., Bidne, K.L., Baumgard, L.H., Keating, A.F., Ross, J.W.   Effects of increased ambient temperature and supplemental altrenogest before pregnancy establishment in gilts. J Anim Sci. 100(2):skac007. <a href="https://doi:10.1093">https://doi:10.1093/jas/skac007</a>.</li> <li>Rudolph TE, Roach CM, Baumgard LH, Ross JW, Keating AF, Selsby JT.   The impact of Zearalenone on heat-stressed skeletal muscle in pigs.  J Anim Sci. 100(8):skac215. <a href="https://doi:10.1093">https://doi:10.1093/jas/skac215</a>.</li> </ol>  Abstracts/Posters/Professional Presentations <ol> <li>Geisert, R. Gene editing provides a tool to investigate the role of conceptus factors involved in the establishment of pregnancy.  Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)</li> <li>Kerns, K.   Capacitation Patterns.  Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)</li> <li>Knox, R.   Follicle Development in Pigs:  State of the Art.  Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)</li> <li>Loucks, A., Hines, E.A.   Processed soy ingredients may adequately replace soybean meal in sow transition diets.  Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium.  Abst. 36. (Poster & Flash talk)</li> <li>Miles, J.   Mechanisms regulating the initiation of porcine conceptus elongation. Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)</li> <li>Miles, J.R., Rempel, L.A., Snider, A.P., Pannier, A.K. 2023 Characterization of mRNA expression for key glycerophospholipid and sphingolipid enzymes within trophectoderm and embryonic disc during initiation of porcine conceptus elongation. Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium.  57. (Poster) </li> <li>Nordell*, N.E., C.J. Bernhard, M. Wegner*, R. Metcalf*, J. Rupert*, D. Lagundžin, Sharma, K.G. Sharp, T.J. Safranski, M.C. Lucy, Desaulniers, A.T. 2023. In utero exposure to maternal heat stress impairs Leydig cell development and function in the boar. ASAS Midwest Animal Science. Madison, WI. (Oral presentation)</li> <li>Nordell, N.E., C.J. Bernhard, M. Wegner, R. Metcalf, J. Rupert, D. Lagundžin, K. Sharma, K.G. Sharp, T.J. Safranski, M.C. Lucy, Desaulniers, A.T. 2022. In utero heat stress impairs Leydig cell development and function in a porcine model. 19th Annual Gilbert S. Greenwald Symposium on Reproductive and Developmental Sciences, Kansas City, MO. Abst 13.  (Poster)</li> <li>Parrish, J.J., Gall, T., Wilson, M., Parsley, M., Ballard, M.   Protected fish oil supplement to boars and the impact on sperm production and spermatogenesis.  ASAS Midwest Animal Science. Madison, WI.  (Oral presentation)</li> <li>Studer, J., Kiefer, Z.E., Koester, L., Johnson, E., Baumgard, L.H., Schmitz-Esser, S., Greiner, L.L., Keating, A.F., Farkas, A., Galina-Pantoja, L., Vonnahme, K.A., Ross, J.W.   Metabolites in Insulin Resistance and Metabolic Syndrome Altered in Sows Suffering from Postpartum Dysgalactia Syndrome.  Proceedings of the Society for the Study of Reproduction 56th Annual Meeting, Ottawa, Canada.  Abst P489 (Poster) </li> <li>Taechamaeteekul, P., Sang-Gassanee, K., Lin H.Y., Collell, M., Komolpis, K., Knox, R.V., Tummaruk, P. 2023 A clinical application of serum progesterone strip test to determine reproductive status in replacement gilts. Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium. 28. (Poster)</li> <li>White, B.   Gonadal steroidogenesis in boars and gilts with reduced endogenous levels of GnRH-II receptor.  Proceedings of the 11th International Conference on Pig Reproduction, Ghent, Belgium.  (Invited presentation)</li> <li>Zigo, M., Kerns, K., Ahlering, P., Sutovsky, P.   Comparison of Human and Porcine Sperm Zincoproteomes with Focus on Clinical and Evolutionary Implications.  Proceedings of the Society for the Study of Reproduction 56th Annual Meeting, Ottawa, Canada.  Abst P456 (Poster)  </li> </ol> Multi-station Funded Grants (Active): <ol> <li>NIH, R01HD095841, “Accumulation, Storage, and Release of Sperm in the Oviduct” Miller1, D. (PD), Prather2, R,. Sutovsky2, P. (1University of Illinois; 2University of Missouri)</li> <li>USDA-AFRI-NIFA Grant #2021-67015-34416, $500,000, Jul 1, 2021 – Oct 1, 2024, “Influence of Maternal and Embryonic Extracellular Vesicles on the Initiation of Porcine Conceptus Elongation” Pannier1, A.K. (PD), Miles2, J.R. (Co-PD), Rempel2, L.A. (1University of Nebraska; 2USDA, ARS, USMARC)</li> <li>USDA-AFRI-NIFA Grant #2022-08916, $999,618, May 1, 2023 – May 1, 2027, “Identifying AMR gene reservoirs and bacterial host-AMR gene associations to identify bacterial host range of AMR genes in swine production systems” Fernando1, S., Burkey1, T., Miller1, P., Schmidt2, A., Anderson2, C., Oliver3, W., Rempel3, L., Wells3, J. (1University of Nebraska; 2Iowa State University; 3USDA, ARS, USMARC)</li> <li>USDA-AFRI-NIFA Grant #2023-XXXXX, $650,000, May 1, 2023 – May 1, 2026, “Enhancing boar fertility in the face of climate change through the mitigation of in utero heat stress” Desaulniers1, A.T. (PD), Lents2, C., White1, B., Brito3, L, Johnson4, J. (1University of Nebraska; 2USDA, ARS, USMARC, 3Purdue University; 4USDA, ARS, West Lafayette)</li> </ol>

Impact Statements

• In support of Objective 1, 3, 4, and 8 – Initiated a new collaborative USDA-AFRI-NIFA grant: Enhancing boar fertility in the face of climate change through the mitigation of in utero heat stress (Nebraska, USDA-ARS-USMARC, Indiana, and USDA-ARS- West Layfette).