12/29/2009

Ando A, Uenishi H, Kawata H, Tanaka M, Shigenari A, Flori L, Chardon P, Lunney JK, Kulski JK, Inoko H. 2008. Microsatellite diversity and crossover regions within homozygous and heterozygous SLA haplotypes of different pig breeds. Immunogenetics. 60: 399-407.

Beura, L. K., Sarkar, S. N., Kwon, B. J., Subramaniam, S., Jones, C., Pattnaik, A. K., and Osorio, F. A. (2010). Porcine Reproductive and Respiratory Syndrome Virus nonstructural protein nsp1b modulates host immune response by antagonizing IRF3 activation. J. Virology In press.

Boyd P, Hudgens E, Loftus JP, Tompkins D, Wysocki M, Kakach L, LaBresh J, Baldwin CL, Lunney JK. 2009. Expressed gene sequence and bioactivity of the IFN³-response chemokine CXCL11 of swine and cattle. Vet. Immunol. Immunopathol. Submitted.

Brockmeier, S. L., K. M. Lager, M. J. Grubman, D. E. Brough, D. Ettyreddy, R. E. Sacco, P. C. Gauger, C. L. Loving, A. C. Vorwald, M. E. Kehrli, Jr., and H. D. Lehmkuhl. 2009. Adenovirus-mediated expression of interferon-alpha delays viral replication and reduces disease signs in swine challenged with porcine reproductive and respiratory syndrome virus. Viral Immunol. 22:173-180.

Brown, E., S. Lawson, C. Welbon, M. P. Murtaugh, E. A. Nelson, J. J. Zimmerman, R. R. R. Rowland, Y. Fang. 2009. Antibody response of nonstructural proteins: implication for diagnostic detection and differentiation of Type I and Type II porcine reproductive and respiratory syndrome virus. Clinical and Vaccine Immunology. 16(5):628-35.

Butler, J.E., P. Weber, N. Wertz and K.M. Lager. 2008. Porcine reproductive and respiratory syndrome virus (PRRSV) subverts development of adaptive immunity by proliferation of germline-encoded B cells with hydrophobic HCDR3s. J. Immunol. 180: 2347-2356.

Calzada-Nova, G., Schnitzlein, W., Husmann, R., Zuckermann, F.A. 2009. Characterization of the cytokine and maturation responses. of pure populations of porcine plasmacytoid dendritic cells to porcine viruses and toll-like receptor agonists. Vet. Imm. Immunopath. doi: 10.1016/j.vetimm.2009.10.26.
Cano JP, Dee SA Murtaugh MM, and Morrison RB. Infection dynamics and clinical manifestations following experimental inoculation of gilts at 90 days of gestation with porcine reproductive and respiratory syndrome virus. . Can J Vet Res (Accepted for publication).

Cano, J.P., S.A. Dee, M.P. Murtaugh, A. Rovira, and R.B. Morrison. 2008. Infection dynamics and clinical manifestations following experimental inoculation of gilts at 90 days of gestation with a low dose of porcine reproductive and respiratory syndrome virus. Can. J. Vet. Res. 73:303-307.

Chang C-C, Yoon K-J, Zimmerman JJ. 2009. Persistent porcine reproductive and respiratory syndrome virus (PRRSV) infection in pigs does not require significant genetic change. J Swine Health Prod 17(6):318324.

Chen Z, Zhou X, Lunney JK, Lawson S, Sun Z, Brown E, Christopher-Hennings J, Knudsen D, Nelson EA. Fang Y. 2009. Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication but play an important role in viral pathogenesis. J Gen Virology. Epub. 11/18/09.

Chen, Z, S. Lawson, Z. Sun, X. Zhou, X. Guan, J. Christopher-Hennings, E. A. Nelson, Y. Fang Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virology (accepted).

Chen, Z., X. Zhou, J. K. Lunney, S. Lawson, Z. Sun, E. Brown, J. Christopher-Hennings, D. Knudsen, E. Nelson, Y. Fang. Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication but play an important role in modulation of host immune response. J. Gen. Virology (accepted).

Cruz, J.L.G., Zuñiga, S., Sanchez, C.M., Ceriani, J.E., Urniza, A., Plana-Duran, J. and Enjuanes L. Immunogenicity of a TGEV-based vector expressing porcine reproductive and respiratory syndrome virus GP5 and M proteins. Vaccine. Submitted.

Das, P. B., Dinh, P. X., Ansari, I. H., de Lima, M., Osorio, F. A., and Pattnaik, A. K. (2010). The Minor Envelope Glycoproteins GP2a and GP4 of Porcine Reproductive and Respiratory Syndrome Virus Interact with the Receptor, CD163. J. Virology In press.

Deendayal Patel, David A. Stein, and Yan-Jin Zhang: Morpholino Oligomer-Mediated Protection of Porcine Pulmonary Alveolar Macrophages from Arterivirus-Induced Cell Death. Antiviral Therapy 2009. In Press.

de Lima, M. Ansari, I. H., Das, P. B., Ku, B., Martinez-Lobo, F. J., Pattnaik, A. K., and Osorio, F. A. (2009). GP3 is a Structural Component of the PRRSV Type II Virion. Virology, 390:31-36.

de Abin, M.F, G. Spronk, M. Wagner, M. Fitzsimmons, J. Abrahante, and M.P. Murtaugh. 2009. Comparative infection efficiency of porcine reproductive and respiratory syndrome virus field isolates on MA104 cells and porcine alveolar macrophages. Can. J. Vet. Res. 73:200-204.

Dee SA, Otake S, Oliviera S and Deen J. Evidence of long distance airborne spread of porcine reproductive and respiratory syndrome virus and Mycoplasma hyopnuemoniae. Vet Res 2009, 40(4)39.

Dee SA, Pitkin AN and Deen J. Evaluation of alternative strategies to MERV 16-based air filtration systems for reduction of the risk of airborne spread of porcine reproductive and respiratory syndrome virus. Vet Microbiol doi:10.1016/j.vetmic.2009.03.019.

Du, Y., Zuckermann, FA, and Yoo, D. 2009. Myristoylation of the small envelope protein of porcine reproductive and respiratory syndrome virus is non-essential for virus infectivity but negatively affects its growth. Virus Res. (submitted)
Ellingson JS, Wang, Y., Layton, S., Ciacci-Zanella, J., Roof, M. B., and Faaberg, K. S. Vaccine efficacy of porcine reproductive and respiratory syndrome virus chimeras. Vaccine, submitted.

Fang, Y., J. Christopher-Hennings, E. Brown, H. Liu, Z. Chen, S. Lawson, R. Breen, T. Clement, X. Gao, J. Bao, D. Knudsen, R. Daly and E.A. Nelson. 2008. Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: implications for future recombinant marker vaccine development. J. Gen. Virol. 89:3086-3096.

Gaudreault, N, RR Rowland, CR Wyatt. 2009. Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus. In press Archiv Virol.

Gudmundsdottir I, Risatti GR. Infection of porcine alveolar macrophages with recombinant chimeric porcine reproductive and respiratory syndrome virus: effects on cellular gene transcription and virus growth. Virus Res. 2009 Oct;145(1):145-50.

Lunney JK. 2008. Genetics of Infectious Disease Resistance in Animals. Proceedings American College of Veterinary Pathologists Meeting 2008. P. 240-242.

Tuggle CK, Wang YF, Couture OP, Qu L, Uthe JJ, Kuhar D, Lunney JK, D. Nettleton D, J.C. Dekkers JC, Bearson SMD. 2008. Computational Integration of Structural and Functional Genomics Data across Species to Develop Information on Porcine Inflammatory Gene Regulatory Pathway. Dev Biol (Basel). 132: 105-13.

Lunney JK, Ho C-S, Wysocki M, Smith DM. 2009. Molecular genetics of the swine major histocompatibility complex, the SLA complex. Dev Comp Immunol. 33: 362-74.

Lunney JK, Fritz ER, Reecy JM, Kuhar D, Prucnal E, Molina R, Christopher-Hennings J, Zimmerman J, Rowland RRR. 2009. Interleukin-8, interleukin-1b and interferon-g levels are linked to PRRS virus clearance. Viral Immunology. In Revision.

Han J, Rutherford, M. S., and K. S. Faaberg. 2009. Porcine reproductive and respiratory syndrome virus nsp2 cysteine protease domain possesses both trans- and cis-cleavage activities. J. Virol. 83:9449-9463.

Han, J., M. S. Rutherford, and K. S. Faaberg. Proteolytic products of the porcine reproductive and respiratory syndrome virus nsp2 replicase protein. J. Virol., submitted.

He D., C.C. Overend, R.J. Maganti, J. Ambrogio, , M.J. Grubman and A.E. Garmendia 2009. Marked differences between MARC-145 cells and alveolar macrophages in IFN ²-induced activation of antiviral state against PRRSV. Submitted to Vet Imm Immunopath

Hermann JR, Brockmeier SL, Yoon KJ, Zimmerman JJ. 2008. Detection of respiratory pathogens in air samples from acutely infected pigs. Can J Vet Res 72:367-370.

Hermann JR, Muñoz-Zanzi CA, Zimmerman JJ. 2009. A method to provide improved dose-response estimates for airborne pathogens in animals: An example using porcine reproductive and respiratory syndrome virus. Vet Microbiol 133:297-302.

Hermann JR, Zimmerman JJ. 2008. Analytical sensitivity of air samplers based on uniform point source exposure to airborne porcine reproductive respiratory syndrome virus and swine influenza virus. Can J Vet Res 72:440-443.

Ho C-S, Lunney JK, Franzo-Romain MH, Martens GW, Lee Y-J, Lee J-H, Wysocki M, Rowland RRR, Smith DM. 2009. Molecular characterization of swine leukocyte antigen (SLA) class I genes in outbred pig populations. Animal Genetics. 40: 468-78.

Ho C-S, Lunney JK, Ando A, Rogel-Gaillard C, Lee J-H, Schook LB, Smith DM. 2009. Nomenclature for factors of the SLA system, update 2008. Tissue Antigens. 73: 307-315.

Ho, C-S, Y-J Lee, JK Lunney, MH Franzo-Romain, GW Martens, J-H Lee, M Wysocki, RRR Rowland, DM Smith. 2009. Molecular characterization of swine leukocyte antigen (SLA) class I genes in outbred pig populations". Accepted for publication Anim Genetics.

Holtkamp DJ, Yeske PE, Polson DD, Melody JL, Philips RC. 2009. A prospective cohort study evaluating duration of breeding herd PRRS virus-free status and its relationship with measured risk. Prev. Vet. Med. (submitted).

Holtkamp DJ., Yu L, Polson DD, OConnor A. 2009. External and internal biosecurity factors associated with the occurrence of clinical PRRS breaks. Vet Res (submitted).

Huang YW, Fang Y, Meng XJ. Identification and characterization of a porcine monocytic cell line supporting porcine reproductive and respiratory syndrome virus (PRRSV) replication and progeny virion production by using an improved DNA-launched PRRSV reverse genetics system. Virus Res. 2009 Oct;145(1):1-8.

Huang YW, Dryman BA, Li W, Meng XJ. Porcine DC-SIGN: molecular cloning, gene structure, tissue distribution and PRRSV binding characteristics. Dev Comp Immunol. 2009 Apr;33(4):464-80.

Jar, A.M., Osorio, F.A., Lopez, O.J. 2009 Mouse x pig chimeric antibodies expressed in baculovirus retain the same properties of their parent antibodies. Biotechnology Progress Mar-Apr;25(2):516-23.

Jacobs AC, Hermann JR, Muñoz-Zanzi C, Prickett JR, Roof MB, Zimmerman JJ. 2010. Thermostability of porcine reproductive and respiratory syndrome virus in solution. J Vet Diagn Invest 22: (in press).

Kim W-I, Kim J-J, Cha S-H, Yoon K-J. 2008. Different biological characteristics between wild-type PRRS viruses and vaccine viruses and identification of the corresponding genetic determinants. J Clin Microbiol 46:1758-1768.

Kim W-I, Yoon K-J. 2008. Molecular assessment of the role of envelope-associated structural proteins in cross neutralization among different PRRS viruses. Virus Genes 37:380-391.

Jung, K., G.J. Renukaradhya, K.P. Alekseev, Y. Fang, Y. Tang, and L.J. Saif (2009). Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J. Gen. Virol. 90:2713-23.

Kim, DY, TJ Kaiser, K Horlen, ML Keith, LP Taylor, R Jolie, JG Calvert, RR Rowland. 2008. Insertion and deletion in a non-essential region of the nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome (PRRS) virus: effects on virulence and immunogenicity. In press Virus Genes.

Klinge, K.L., E.M. Vaughn, M.B. Roof, E.M. Bautista, and M.P. Murtaugh. 2009. Age-dependent resistance to Porcine reproductive and respiratory syndrome virus replication in swine. Virol. J. 6:177-187.

Loving, C. L., S. L. Brockmeier, A. L. Vincent, K. M. Lager, and R. E. Sacco. 2008. Differences in clinical disease and immune response of pigs challenged with a high-dose versus low-dose inoculum of porcine reproductive and respiratory syndrome virus. Viral Immunol. 21:315-325.

Lunney JK, Fritz ER, Reecy JM, Kuhar D, Prucnal E, Molina R, Christopher-Hennings J, Zimmerman J, Rowland RRR. 2010. Interleukin-8, interleukin 1² and interferon-³ levels are linked to PRRS virus clearance. Viral Immunol (in press).

Metwally, S., F. Mohamed, T. Burrage, M. Prarat, K. Moran, A. Bracht, G. Mayr, M. Berninger, K. S. Faaberg, L. Koster, L.T. Thanh, V.L. Nguyen, M. Reising, S. Swenson, J. Lubroth and C. Carrillo. Pathogenicity and molecular characterization of emerging porcine reproductive and respiratory syndrome virus in Vietnam 2007. Transboundary and Emerging Diseases, submitted.

Miller, L.C., Lager, K.M. and Kehrli Jr., M.E. 2009. Effect of porcine reproductive and respiratory syndrome virus infection of porcine alveolar macrophages on Toll-like receptors elicitation of type I interferon responses. Clinical and Vaccine Immunology 16:360-365.

Molina, R.M., E.A. Nelson, J. Christopher-Hennings, R. Hesse, R.R. Rowland, J.J. Zimmerman, 2009. Evaluation of the risk of PRRSV transmission via ingestion of muscle from persistently-infected pigs. Transboundary and Emerging Diseases 56:1-8.

Molina R, S.-H. Cha, W Chittick, S. Lawson, M.P. Murtaugh, E.A. Nelson, J Christopher-Hennings, K.-J. Yoon, R. Evans, R.R.R. Rowland and J.J. Zimmerman. 2008. Immune response against porcine reproductive and respiratory syndrome virus during acute and chronic infection. Veterinary Immunology and Immunopathology.

Molina, R.M., W. Chittick, E.A. Nelson, J. Christopher-Hennings, R.R.R. Rowland and J.J. Zimmerman. 2008. Diagnostic performance of assays for the detection of anti-PRRSV antibodies in porcine muscle transudate (meat juice) samples. J. Vet. Diagn. Invest. 20:735-743.

Mohammadi, H, S Sharif, RRR Rowland, D. Yoo. 2009. The lactate dehydrogenase-elevating virus capsid protein is a nuclear-cytoplasmic protein. Arch Virol. 154:1071-1080.

Murtaugh, M.P., C.R. Johnson, Z. Xiao, R.W. Scamurra, and Y. Zhou. 2009. Species specialization in cytokine biology: is interleukin-4 central to the TH1-TH2 paradigm in swine. Develop. Comp. Immunol. 33:344-352.

Opriessnig T, Patterson AR, Madson DM, PalM, Rothschild M, Kuhar D, Lunney JK, Juhan NM, Meng XJ, Halbur PG. 2009. Difference in severity of porcine circovirus type 2 (PCV2)-induced pathological lesions and disease between Landrace and Pietrain pigs. J. Animal Science. 87: 1582-90.

Opriessnig T, Madson DM, Prickett JR, Kuhar D, Lunney JK, Elsener J, Halbur PG. 2008. Effect of porcine circovirus type 2 (PCV2) vaccination on porcine reproductive and respiratory syndrome virus (PRRSV) and PCV2 coinfection. Vet. Microbiol. 131: 103-14.

Patton, J. B., R.R. Rowland, D. Yoo, and K.C. Chang. 2009. Modulation of CD163 receptor expression and replication of porcine reproductive and respiratory syndrome virus in porcine macrophages. Virus Res. 140:161-171.

Pei, Y., D.C. Hodgins, J. Wu, S.K.W. Welch, J.G. Calvert, G. Li, Y. Du, C. Song, and Yoo, D.. 2009. Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type-2 capsid expressed from dedicated subgenomic RNAs. Virology 389:91-99.

Pieters M, Dee SA, Fano E and Pijoan C. An assessment of the duration of Mycoplasma hyopneumoniae infection in an experimentally infected population of pigs. Vet Microbiol 2009;143:261-264.

Pitkin AN, Deen J and Dee SA. Further assessment of fomites and personnel as vehicles for the mechanical transport and transmission of porcine reproductive and respiratory syndrome virus. Can J Vet Res (Accepted for publication).

Pitkin AN, Deen J and Dee SA. Use of a production region model to assess the airborne spread of porcine reproductive and respiratory syndrome virus. Vet Microbiol 2009;136:1-7.

Pitkin AN, Otake S, Deen J, Moon RD, Dee SA. Further assessment of houseflies (Musca domestica) as vectors for the mechanical transport and transmission of porcine reproductive and respiratory syndrome virus under field conditions. Can J Vet Res 2009;73:91-96.

Prickett J, Kim W-I, Simmer R, Yoon K-J, Zimmerman JJ. 2008. Surveillance of commercial growing pigs for PRRSV and PCV2 infections using pen-based oral fluid sample: a pilot study. J Swine Health Prod 16(2):86-91.

Prickett J, Simer R, Christopher-Hennings J, Yoon K-J, Evans RB, Zimmerman JJ. 2008. Detection of porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: A longitudinal study under experimental conditions. J Vet Diagn Invest 20:156-163.

Ran ZG, Chen XY, Guo X, Ge NX, Yoon K-J, Yang HC. 2008. Recovery of viable porcine reproductive and respiratory syndrome virus from an infectious clone containing partial deletion within Nsp2-encoding region. Arch Virol 153:899-907.

Renukaradhya, G.J., K.P. Alekseev, K. Jung, Y. Tang, Y. Fang, and L.J. Saif (2009). Altered immune responses to porcine respiratory coronavirus in pigs previously infected with porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopath. (In review).

Rosenfeld, P., P.V. Turner, J.I. MacInnes, E. Nagy, and D. Yoo. 2009. Evaluation of porcine reproductive and respiratory syndrome virus replication in the laboratory rodents. Can. J. Vet. Res. 73: 313-318.

Sang, Y, P Ruchala, R Lehrer, CR Ross, RRR Rowland, Frank Blecha. 2009. Antimicrobial Host Defense Peptides in an Arteriviral Infection: Differential Peptide Expression and Virus Inactivation. Viral Immun. Accepted for publication.

Sang, Y, C Ross, RR Rowland, F Blecha. 2008. Toll-like receptor 3 (TLR3) activation decreases porcine arterivirus infection. Viral Immunol 21:303-313.

Sang, Y, J Yang, C Ross, RRR Rowland, and F Blecha, 2008. Molecular identification and functional expression of porcine Toll-like receptor (TLR) 3 and TLR7. In press Vet Immunol Immunopath.

Song, C., R. Lu, D. Bienzle, H.C. Liu, and D. Yoo. 2009. Interaction of porcine reproductive and respiratory syndrome virus nucleocapsid protein with the inhibitor of MyoD family-a domain containing protein. Biol. Chem. 390: 215-223.

Spilman, M.S., C. Welbon, E.A. Nelson and T. Dokland. 2009. Cryo-electron tomography of porcine reproductive and respiratory syndrome virus (PRRSV): organization of the nucleocapsid. J. Gen. Virol. 90:527-535.

Steibel JP, Wysocki M, Lunney JK, Ramos AM, Hu Z-L, Rothschild MF, Ernst CW. 2009. Validation of the Swine Protein-Annotated Oligonucleotide Microarray. Animal Genetics. 40: 883-893.

Trible BR, Kerrigan, M., Faaberg, K. S., and R. R.R. Rowland. Identification of an immunodominant region the PCV2 capsid protein recognized by naturally infected and vaccinated pigs. Journal of General Virology, submitted.

Vashisht, K., Erlandson, K.R., Firkins, L.D., Zuckermann, F.A., Goldberg, T.L. 2008. Evaluation of contact exposure as a method for acclimatizing growing pigs to porcine reproductive and respiratory syndrome virus. J Am Vet Med Assoc. 232:1530-5.

Vashisht, K., Goldberg, T.L., Husmann, R.J., Schnitzlein, W., Zuckermann, F.A. 2008. Identification of immunodominant T-cell epitopes present in glycoprotein 5 of the North American genotype of porcine reproductive and respiratory syndrome virus. Vaccine. 26:4747-53.

Vincent, A. L., Lager, K. M., Faaberg, K. S., Harland, M. L., Zanella, E., Ciacci-Zanella, J., Kehrli, Jr., M. E., Janke, B. H., Klimov, A. Susceptibility of Pigs to Pandemic 2009 A/H1N1 Influenza Virus. Plos Pathogens, submitted.

Wenjun Ma, AL Vincent, KM Lager, BH Janke, SC Henry, RRR Rowland, RA Hesse, JA Richt. 2009. Identification and characterization of a highly virulent triple reassortant H1N1 swine influenza virus in the United States. Virus Genes, in press.

Wu, J., J. Li, F. Tian, J. Shi, S. Ren, Z. Lan, X. Zhang, D. Yoo, and J. Wang. 2009. Porcine high fever disease: genetic variation and pathogenicity of porcine reproductive and respiratory syndrome virus in China. Arch. Virol. 154: 1579-1588.

Xue Han, Sumin Fan, Deendayal Patel, and Yan-Jin Zhang: Enhanced Inhibitory Effect on PRRSV Replication by Combination of Two Morpholino oligomers. Antiviral Research 2009. 82:59-66.

Yaeger M, Karriker L, Layman L, Halbur P, Huber G, Van Hulzen K. 2009. Survey of disease pressures in twenty-six niche herds in the Midwestern United States. J Swine Health Prod. 2009 17(5): 256-263.

Yue F, Cui S, Zhang C, Yoon K-J. 2009. A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. Virus Genes (in press)



2. List book chapters or monographs
None


3. List abstracts or proceedings

Beura L, Sarkar S , Kwon BJ, Subramaniam S, Jones C, Pattnaik AK, Osorio FA. Porcine reproductive and respiratory syndrome virus non structural protein 1 beta inhibits host innate immune response by antagonizing IRF3 activation. Proceedings of the 28th Annual Meeting American Society for Virology (25th ASV), Vancouver, BC, July 11-15, 2009 (Workshop 33-6).

Butler, J.E. 2009 A comparative study of PRRSV, PCV-2 and SIV infections in germfree isolator piglets. Presented at 5th International Veterinary Vaccine Conference (5th IVVDC), Madison WI. July 23.

Carley D, Ramamoorthy S, Opriessnig T, Wong C, Tobin G, Yoon KJ, Halbur PG, Messel R, Nara PL. 2009. Evaluation of the PRRSV antibody response following vaccination with a proprietary autogenous vaccine. Proceedings, Summer Scholar Program.

Carley D, Ramamoorthy S, Opriessnig T, Wang C, Tobin G, Yoon KJ, Halbur PG, Messel R, Nara PL. August 2009. Evaluation of the PRRSV antibody response following vaccination with a proprietary autogenous vaccine. Research Day, College of Veterinary Medicine, Iowa State University. Ames, IA.

Carmichael BA, Polson DD, Torremorell M, Holtkamp DJ. 2009. Pre-colostral stillborn piglet blood sampling procedure when a PRRSV positive sow herd is being monitored for time-to-negative interval. 40th AASV Annual Meeting. Dallas, Texas.

Cha S-H, Dorman KS, Kim W-I, Yoon KJ. 2008. Viral recombination among field isolates of PRRSV type 2  implication for molecular epidemiology. PRRS Symposium.

Chen Z, X Zhou, D Kuhar, S Lawson, J Lunney, Y Fang. 2008. Effect of PRRSV nsp2 epitope deletion mutants on the induction of cytokine response in porcine alveolar macrophages. 2008 CRWAD & 2008 PRRSV Symp.

Chen Z, X Zhou, S Lawson, E Brown, R Breen J. Christopher-Hennings, E Nelson, Y Fang. 2008. Expression of foreign proteins in replicase gene regions of porcine reproductive and respiratory syndrome virus. CRWAD & 2008 PRRSV Symp.

Chitko-McKown, C.G., Miller, L.C., Lager, K.M. and Kehrli Jr., M.E. Effects of PRRSV infection on TLR-dependent induction of NOS [abstract]. 2008 Conference of Research Workers in Animal Diseases (CRWAD), December 7-9, 2008, Chicago, IL.

Chitko-McKown, C.G., Chapes, S.K., Miller, L.C., and Green, B.T. Characterization of the porcine monocyte-derived cell lines Cdelta2+ and Cdelta2-. 41st Annual Meeting of the Society for Leukocyte Biology, November 6-8, 2008, Denver, CO.

Cutler T, Hoff S, Wang C, Warren K, Zhou F, Qin Q, Miller C, Ridpath J, Yoon K-J, Zimmerman J. 2009. UV254 Inactivation of Selected Viral Pathogens. 52st Annual Conference, American Association of Veterinary Laboratory Diagnosticians. San Diego, California, p. 124.

Cutler T, Kittawornrat A, Hoff S, Wang C, Zimmerman J. 2009. Median infectious dose (ID50) of PRRSV isolate MN-184 for young pigs via aerosol exposure. 52st Annual Conference, American Association of Veterinary Laboratory Diagnosticians. San Diego, California, p. 110.

Cruz, J. L. G., Zúñiga, S., Sánchez, C. M., Ceriani, J. E., Plana, J., and Enjuanes, L. 2008. Design of a recombinant TGEV vector to protect against porcine reproductive and respiratory syndrome. EuroPRRSnet Workshop. Combating PRRS in Europe.

Cruz, J. L. G., Zúñiga, S., Sánchez, C. M., Urniza, A., Bru, T., Ceriani, J. E., Plana, J., and Enjuanes, L. 2008. Construction of a TGEV vector to protect against porcine reproductive and respiratory syndrome. 2008 PRRS Symposium.

Cruz, J. L. G., Zúñiga, S., Sánchez, C. M., Ros, S., Juanola, S., Plana, J., and Enjuanes, L. 2009. Design of a TGEV vector to protect against porcine reproductive and respiratory syndrome. 2009 PRRS Symposium.

Dee SA and Otake S. Investigation of alternative strategies for aerosol biosecurity for PRRSV. CRWAD, Chicago, Il, December 2008.

Dee SA and Otake S. Use of a production region model to evaluate issues regarding the aerobiology of PRRSV and Mycoplasma hyopneumoniae. CRWAD, Chicago, Il, December 2008.

Dee SA, AN Pitkin, Deen J. Alternative strategies for aerosol biosecurity for PRRSV. 2008 Pijoan Intl Symp on Swine Dis Erad St. Paul, September, 2008.

Dee SA, Otake S, Deen J. Use of a production region model to evaluate the transmission and biosecurity of PRRS and Mycoplasma hyopneumoniae. AASV, Dallas, Tx, March 2009.

Dee SA, Pitkin AN, Otake S and Deen J. Transmission of EP and PRRS. PIC Veterinary Conference, Stratford-upon-Avon, England, February 2009.

Dion KR, Dau D, Delks AM, Hammer M, Holtkamp DJ. 2009. Feed medication protocol comparison in a PRRSv unstable nursery flow. 40th AASV Meeting. Dallas, Texas.

Dixon PM, Yoon K-J. 2008. Estimating the mutation rate when not all mutations are detected. Proceedings, Iowa State University Fall Conference on Statistics in Biology.

Dwivedi V, C. Manickam, R. Patterson, K. Dodson, and G. J. Renukaradhya. Steps towards development of a novel mucosal vaccine to PRRSV. Fourth International Scholar Research Exposition, The Ohio State University, November 19th, 2009.

Dwivedi V, C. Manickam, R. Patterson, K. Dodson, and G. J. Renukaradhya. Development of a novel mucosal vaccine to protect against porcine reproductive and respiratory syndrome in pigs. 2009 PRRSV Symposium.

Faaberg KS, J. Han, K. M. Lager, M. E. Kehrli, Jr., and M. S. Rutherford. 2008. PRRSV strain VR-2332 nsp2 deletion mutants attenuate clinical symptoms in swine. XIth International Symposium on Nidoviruses, P43, Oxford, Great Britain.

Faaberg, K. S. 2008. State of the Art: Lessons learned through porcine reproductive and respiratory syndrome virus (PRRSV) recombinant technology. International Congress of Virology, 675, Istanbul, Turkey.

Fang, Y. 2008. Structural and Function of PRRSV nonstructural proteins: where are we at? 2008 PRRSV Symp.

Fritz E, Hu Z, Lunney J, Reecy J. 2008. The PHGC Database: management of large data sets. Intnl PRRS Symp. #324. 12/0.

Guo, B., Faaberg, K. S., Lager, K., and M. E. Kehrli, Jr. 2009. Genetic stability of PRRSV VR-2332 nsp2 deletion mutants in swine. 28th Annual Meeting of the American Society for Virology, W33-12, Vancouver, Canada.

Haley CA, Wagner B, Murtaugh MP. 2009. Estimating the sensitivity and specificity of a new ELISA test for porcine circovirus 2 exposure using a study pseudo gold standard and latent-class analysis. Proc AASV. pp 255-261.

Harms PA, Holtkamp DJ, Quirk Z. 2009. Management of, and costs associated with, false positive when monitoring presumed PRRS-negative herds. 40th AASV Annual Meeting, Pre-Conference Seminar, Managing PRRS Introduction into High Risk Populations. Dallas, Texas.

Holtkamp DJ, Melody JL, Burkgren TJ. 2008. Update On The AASV Production Animal Disease Risk Assessment Program (PADRAP) and the New Web-based Application. 20th IPVS Meetings. Durban, South Africa. June. p. 01.66.

Holtkamp DJ, Melody JL, MacDougald D. 2008. A comparison of PRRSV risks for Canadian and U.S. breeding herds. 20th IPVS Meetings. Durban, South Africa. June. p. 01.77.

Holtkamp DJ, Polson DD. 2008. PRRS herd classification: Can we speak the same language? Carlos Pijoan International Symposium: New Solutions to Old Problems. Pre-Conference Workshop 2006 Allen D. Leman Swine Conference. St. Paul, Minnesota.

Hesse, R, R Rowland. 2008. Circovirus Vaccination Decisions: Herd Profiling and Next Generation Diagnostic Testing. 2008 Leman Swine Conference, St Paul, MN.

Hiep Vu , Kwon BJ, Yoon KJ, Laegreid W, Pattnaik AK and Osorio FA. Analysis of the aberrant immune response induced by a PRRSV type 2 isolate naturally lacking glycan residues in two envelope glycoproteins. 2009 IPRRSS (#84) and 2009 CRWAD (poster # 64).

Hicks, J., D. Yoo, and H.C. Liu. 2008. Identification of domains of PRRS virus GP5 and M proteins that interact with the host Snap-associated protein SNAPIN. 2008 IPRRSS.

Hu J, Meng XJ, and Zhang C. 2008. Purification of native PRRSV virions from cell culture. 2008 PRRS Symposium.

Huang YW, B. A. Dryman, X. J. Meng. 2008. Molecular cloning of porcine DC-SIGN and detection of its potential interaction with porcine reproductive and respiratory syndrome virus. 2008 International PRRS Symposium. 2008 PRRS Symposium.

Huang YW, B. A. Dryman, X. J. Meng. 2008. Molecular cloning of porcine DC-SIGN and detection of its potential interaction with porcine reproductive and respiratory syndrome virus. 2008 International PRRS Symposium. 2008 CRWAD.

Jung K, K. Alekseev, G.J. Renukaradhya, Y. Tang, Y. Fang, P. Lewis, X. Zhang, L.J. Saif. Altered pathogenesis of porcine respiratory coronavirus (PRCV) in the presence of PRRSV infection and their pathologic relationships: Potential effect of preexisting respiratory viral infections on SARS severity. XIV International Congress of Virology, ASV, Cornell University, Ithaca, NY, 11-15 August 2008.

Jung K, K. Alekseev, G.J. Renukaradhya, Y. Tang, Y. Fang, P. Lewis, X. Zhang, L.J. Saif. Altered pathogenesis of porcine respiratory coronavirus (PRCV) subsequent to PRRSV infection: Model for effect of respiratory viral co-infections on SARS severity. 2008 CRWAD.

Karriker L, Bowden J. 2009. PRRS virus vaccination strategies and efficacy. Proceedings of the George A. Young Swine Health and Management Conference. South Sioux City, Nebraska.

Karriker L, Layman L, Yaeger M. 2008. Session 5531: Health challenges in niche production. Conference Notes CD of the 145th AVMA. New Orleans, Louisiana.

Karriker L. 2008. Session 5543: PRRSV: management strategies. Current swine disease trends. Conference Notes CD of the 145th AVMA. New Orleans, Louisiana.

Kim W-I, Bower L, Strait E, Harmon K, Yoon K-J. 2009. Simultaneous detection of multiple pathogens using high-throughput nanoliter real-time PCR. AAVLD.

Kim W-I, Cho Y-I, Harmon K, Madson D, Opriessnig T, Yoon K-J. 2008. Comparison of three extraction methods for the detection of PCV2 and PRRSV in semen. AAVLD.

Kim W-I, Sun D, Cho Y-I, Liu S, Cooper V, Yoon K-J. 2009. Identification of molecular markers for virulence of porcine reproductive and respiratory syndrome (PRRS) virus. AAVLD

Kim W-I, Sun D, Cho Y-I, Liu S, Loynachan AT, Cooper VC, Yoon KJ. 2009. Genetic determinants associated with the virulence of PRRSV in pigs. AASV.

Koziel JA, Yang X, Cutler T, Zhang S, Zimmerman J, Hoff S, Jenks WS, van Leeuwen JH, Laor Y, Ravid U, Armon R. 2008. Treatment of livestock odor and pathogens with ultraviolet photocatalysis. Proc AgEng 2008 International Conference on Agricultural Engineering and Industry Exhibition, (Abstr OP-575). Hersonissos, Greece.

Koziel JA, Yang X, Zhang S, Cai L, Hoff SJ, Leeuwen HJ, Cutler T, Zimmerman J, Jenks WS, Laor Y, Ravid U, Armon R. October 2008. Treatment of livestock odor and pathogens with ultraviolet photocatalysis. Proc 3rd IWA Odour and VOCs Conference. Barcelona, Spain.

Lawson S, Lunney JK, Fang Y, Nelson EA, Christopher-Hennings J. 2009. Development of a rapid, swine-specific microsphere assay to simultaneously detect multiple immune proteins (cytokines) affected by porcine reproductive and respiratory syndrome virus (PRRSV) infection. 2009 Intnl PRRS Symp. and CRWAD 12/09.

Liu S, Kim W-I, Ramamoorthy S, Yoon K-J. 2009. Alternative assays and testing algorithm for confirmation of suspect false positives in a commercial ELISA for PRRSV. AAVLD.

Lunney JK. 2008. Genetics of Infectious Disease Resistance in Animals: Pig and PRRS. Proc. Am Coll Vet Pathol. Meeting. 11/08

Lunney JK. 2009. PRRS Host Genetics Consortium: Current Progress and Potential for Canadian Involvement. Canadian Centre for Swine Improvement meeting, Quebec City, Canada 6/09

Lunney JK, Boyd P, LaBresh J, Kakach L, Wagner B, Tompkins D, Hudgens E, Baldwin C. 2009. Swine Toolkit progress for the US Veterinary Immune Reagent Network. 2009 Intnl PRRS Symp. and CRWAD 12/09.

Lunney JK, Boyd P, Prucnal L, Zarlenga D, LaBresh J, Steffens C, Wagner B, Tompkins D, Hudgens T, C Baldwin C. 2008. Swine Toolkit progress for the US Veterinary Immune Reagent Network. Intnl PRRS Symp. #288 and CRWAD 95P. 12/08

Lunney JK, Reecy J, Rowland RRR. 2009. PRRS Host Genetics Consortium: Current Progress and Potential for Canadian Involvement. Canadian Swine Health Forum 2009: July 7-8, 2009, Saskatoon, SK, Canada.

Lunney JK, Reecy J, Rowland RRR. 2009. Current Progress of US PRRS Host Genetics Consortium. Genomics for Animal Health: Outlook for the Future (EADGENE 2009) meeting, 10/09, Paris, France.

Lunney JK, Rowland RRR, Chen Z, Zhou X, Lawson S, Sun Z, E. Brown E, J. Christopher-Hennings J, Nelson E, Fang Y. 2009. Genetic approaches to reveal immune response pathways and viral antigen targets for novel vaccine design. Intnl Vet Vaccines and Diagnostics Conference (IVVDC 2009), WI. 7/09.

Lunney JK, Steibel JP, Reecy J, Rothschild M, Kerrigan M, Trible B, Rowland RRR. 2009. PRRS Host Genetics Consortium: Current Progress. 2009 Intnl PRRS Symp. and CRWAD 12/09.

Lunney JK, Wysocki M, Steibel JP, Kuhar D, Ernst CW, McCaw M. 2009. Uncovering Genetic Components Involved In Regulating Early Immune Responses To Porcine Reproductive And Respiratory Syndrome (PRRS). PAG2009. PAG-XVII P640. 1/09.

Loruzzo, A., Faaberg, K. S., Killian, M. L., Koster, L., Vincent, A. L. 2009. One step real-time RT-PCR for 2009 pandemic H1N1 matrix gene detection and quantitation in clinical samples. American Association of Swine Veterinarians 2010 Annual Meeting, March 6-9, 2010, Omaha, NE.

Metwally S, C. Carrillo, F. Mohamed, K. Faaberg, M. McIntosh, L. Cox, L. Koster, S. Swenson, T. Burrage, T. Long, T. Beckham, E. Lautner, and J. Lubroth. 2008. Porcine High Fever Disease in Vietnam 2007; PRRS and Other Disease Agents. 51st Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians, Greensboro, NC, USA.

Miller, L.C., Harhay, G.P., Lager, K.M., Kehrli Jr., M.E., Laegreid, W.W. and Neill, J.D. In depth global analysis of gene expression levels in porcine alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus [abstract]. ARK-Genomics Conference 2008: 3rd International Symposium on Animal Functional Genomics, April 7-9, 2008, Edinburgh, U.K. Paper No. ISAFG-P22.p. 38.

Miller, L.C., Chitko-McKown, C.G., Lager, K.M. and Kehrli Jr., M.E. Differential roles of Toll-like receptors in the elicitation of type I interferon responses by alveolar macrophages [abstract]. 2008 International PRRS Symposium, December 5-6, 2008, Chicago, IL.

Molina RM, Cha S-H, Rowland RRR, Christopher-Hennings J, Nelson E, Lunney J, Yoon K-J, Zimmerman JJ. 2008. Factores involucrados en la persistencia del virus de sindrome respiratorio porcino (PRRS). Memorias XXI Congreso Panamericano de Ciencias Veterinarias. Guadalajara, México, pp. 533- 534.

Morrison RB, Davies PD and Dee SA. Regional and national eradication of PRRS. PIC Veterinary Conference, Stratford-upon-Avon, England, February 2009.

Morrison, RB. Update on PRRS elimination in Stevens County, MN. Allen D Leman Swine Conference, preconference workshop. Pp. 67-74.

Murtaugh M. 2009. Update on PRRSV immunology and viral genetics: from hopeless to hopeful. Proc AASV. pp 459-462.

Osorio FA. Worldwide Research Efforts Towards a Broadly Protective and Effective Vaccine against PRRSV. Keynote presentation # 4 at the 2008 IPRRSS.

Osorio FA. PRRSV immunology and vaccines Second Annual CVM Swine Health Initiatives Meeting UIUC, PRRSV protective immunity and immunization, Osorio FA presented at the XVIII Congreso Dia del Porcicultor, Navojoa, Sonora Mexico. 2008.

Otake S, Deen J, Dee SA. New information aerosol transmission and biosecurity for Mycoplasma hyopneumoniae. 2008 Leman Swine Conference.

Otake S, Deen J, Dee SA. Preliminary information from recent research on PRRSV and Mycoplasma hyopneumoniae transmission and biosecurity: Field application of air filters. 2008 Leman Swine Conference.

Pires-Alves, M., Misra, A., Zuckermann, F.A., Laegreid, W. 2009. Comparison of two cell lines for the propagation of PRRSV. 2009 IPRRSS.

Potter, ML, S Dritiz, R Hesse, R Rowland, J Nietfeld, R Oberst. 2008. Porcine Cirovirus Type 2 elimination study. 2008 AASV.

Potter, ML, LM Tokach, SS Dritz, SC Henry, JM DeRouchey, MD Tokach, RD Goodband, JL Nelsen, RR Rowland, RD Hesse, RA Hesse. 2008. Genetic background influences pig growth rate responses to porcine circovirus type 2 (PCV2) vaccines. 2008 KSU Swine Day.

Prickett J, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman J. 2008. PRRSV surveillance  Stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. Proc 89th CRWAD, Abstr #38.

Prickett J, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman J. 2008. PRRSV surveillance  Stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. 2008 IPRRSS, p 26.

Prickett J, Hoffmann P, Main R, Sornsen S, Johnson J, Zimmerman J. 2009. Cost-effective PRRS surveillance. AASV, pp. 467-469.

Prickett J, Hoffmann P, Main R, Stensland W, Yoon K-J, Zimmerman J. 2008. Practical disease surveillance in growing pig populations. Proc 89th CRWAD, Abstr #39.

Prickett J, Hoffmann P, Main R, Stensland W, Yoon K-J, Zimmerman J. 2008. Practical disease surveillance in growing pig populations. Proc 2008 IPRRSS, p 27.

Prickett J, Zimmerman J. 2008. Practical disease surveillance in growing pig populations. Proceedings, Welfare and Epidemiology Conference: Across Species, Across Disciplines, and Across Borders. Ames, Iowa, p. 22.

Prickett J, Zimmerman J. 2009. Practical disease surveillance in growing pig populations. J Appl Anim Welf Sci 12:156.

Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland W, Yoon KJ, Zimmerman J. 2008. PRRSV surveillance - Stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. Proc 51st Annual Conference, American Association of Veterinary Laboratory Diagnosticians. Greensboro, North Carolina, p. 140.

Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland W, Yoon K-J, Zimmerman J. 2008. PRRSV surveillance - stability of diagnostic targets in oral fluid: Sample storage and critical techniques for testing. Proc 47th North Central Conference of Veterinary Laboratory Diagnosticians. Madison, Wisconsin, pp. 8.

Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman JJ. 2008. PRRSV surveillance  stability of diagnostic targets in oral fluid: sample storage and critical techniques for testing. AAVLD.

Prickett JR, Cutler S, Kinyon J, Naberhaus N, Stensland WR, Yoon K-J, Zimmerman JJ. 2008. PRRS surveillance  Stability of diagnostic targets in oral fluid: Sample storage and critical techniques for testing. NCCVLD.

Prickett JR, Hoffmann P, Main R, Sornsen S, Johnson J, Zimmerman J. 2008. Infectious disease surveillance in commercial swine populations. Proceedings 16th Annual Swine Disease Conference for Swine Practitioners, Iowa State University. Ames, Iowa, pp. 40-44.

Prickett JR, Hoffmann P, Main R, Stensland W, Yoon K-J, Zimmerman J. June 2008. Practical disease surveillance in growing pig populations. Proc 47th North Central Conference of Veterinary Laboratory Diagnosticians. Madison, Wisconsin, pp. 10.

Prickett JR, Hoffmann P, Stensland W, Yoon KJ, Zimmerman J. 2008. Practical disease surveillance in growing pig populations. Proc 51st Annual Conference, AAVLD, Greensboro, NC, p. 139.

Renukaradhya GJ, Konstantin Alekseev, Kwonil Jung, and Linda J. Saif. Distorted immune responses in pigs to porcine respiratory coronavirus previously infected with porcine reproductive and respiratory syndrome virus: a respiratory viral co-infection model. 2009 CRWAD.

Reister L, T Clement, E Nelson, J Christopher-Hennings. 2008. Potential mechanical and antiviral methods to insure PRRSV free semen. 2008 CRWAD & 2008 PRRSV Symp.

Rovira A, Abrahante J, Murtaugh M. 2009. Detection of porcine reproductive and respiratory syndrome virus (PRRSV) by reverse transcriptase loop mediated isothermal amplification (RT-LAMP). Proc AASV. pp 109-110.

Rowland RRR, Kerrigan M, Bujuru S, Trible B, Lunney JK. 2009. An infection model for the study of PRRS at the population level. 2009 IPRRSS. 12/09.

Rowland, R, S Henry, S Dritz, R Hesse. 2008. Epidemiology of PCV2 and PCVAD. 2008 AASV.

Rowland, R, R Hesse, K Horlen. 2008. Porcine circovirus vaccine trials: from the laboratory bench to the field. George Young Swine Conference, Sioux City.

Rowland, R. 2008. PRRS vaccines. 2008 Leman Swine Conference, St Paul, MN.

Rowland, R. 2008. Mapping host protective immunity in the PCV2 capsid protein. USDA NRI Prinicipal Investigators Meeting, Chicago.

Sang, Y, P Ruchala, RI Lehrer, CR Ross, RRR Rowland, F Blecha. 2008. Antimicrobial host defense peptides in an arteriviral infection: differential expression and inactivation of PRRSV. 2008 IPRRSS.

Sun D, Kim W, Cho Y, Cooper V, Cha S, Kim S, Choi E, Yoon K. 2009. Role of viral structural proteins in conferring protective immunity against PRRSV and its application to the development of vaccine candidates for broad cross-protection. 2009 AASV.

Sun D, Kim W-I, Cho Y-I, Cha S-H, Kim S-H, Choi E-J, Yoon KJ. 2008. Cross-protection induced by chimeric mutant containing mixed structural genes of two different PRRS viruses. 2008 CRWAD.

Sun D, Kim W-I, Cho Y-I, Yoon KJ. 2008. Attempt to achieve broader cross-protection among PRRS viruses by vaccination: use of chimeric mutant containing mixed structural genes of two different PRRS viruses. 2008 PRRS Symp.

Trible, B, JG Calvert , RRR Rowland. 2008. Expression of enhanced green fluorescent protein (EGFP) in nonstructural protein 2 (nsp2) of PRRSV shows loss of fluorescence without affecting EGFP immunogenicity.

Tuggle CK, Bearson SMD, Uthe JJ, Christian C, Couture O, Demirkale CY, Nettleton D, Lunney JK, Honavar V. 2009. Using transcriptomic data to develop tools for predicting shedding traits in growing pigs. CRWAD 12/09.

Vincent, A. L., Lager, K. M., Faaberg, K. S., Harland, M. L., Zanella, E., Ciacci-Zanella, J., Kehrli, Jr., M. E., Janke, B. H., Klimov, A. Susceptibility of North American Swine to the Novel A/H1N1 Influenza A Virus. Center of Excellence Symposium, Minneapolis, MN.

Vu HLX, M. Brito M, Kim WI, Yoon KJ, Laegreid W, Osorio FA. 2008. Sub-typing PRRSV isolates by means of measurement of cross neutralization reactions. CRWAD.

Vu HLX, M. Brito M, Kim WI, Yoon KJ, Laegreid W, Osorio FA. 2008. Sub-typing PRRSV isolates by means of measurement of cross neutralization reactions. PRRS Symp.

Waddell JM, Melody JL, Holtkamp DJ. 2009. Investigation of associations between risk factors, reported clinical PRRS breaks and reproductive performance in swine breeding herds. Proc. 40th AASV Annual Meeting. Dallas, Texas.

Waddell JT, Polson DD, Holtkamp DJ. 2008. Assessment of changes in breeding herd PRRS site risk scores in a large production system over a three-year period. Proc 20th IPVS Meetings. Durban, South Africa. p. 01.135.

Wong SJ, Lunney JK, Rowland RRR. 2009. Nucleocapsid protein-specific IgG and IgM responses in oral fluids during PRRSV infection. 2009 Intnl PRRS Symp. 12/09.

Wong, SJ, R Hesse, R Rowland. Application of multiplex microsphere immunoassay techniques to the diagnosis of PRRSV and other infectious. 2008 International PRRS Symposium, Chicago.

Wysocki M, SteibelJP, McCaw M, Kuhar D, Ernst CW, Lunney JK. 2008. Uncovering genetic components involved in early regulatory immune response during PRRSV infection. Intnl PRRS Symp. #285 and CRWAD #125. 12/08.

Wu, J., Li, J., Tian, F., Shi, J., Ren, S., Lan, Z., Zhang, X., Niu, Z., Yoo, D., and Wang, J. 2008. Genetic variation and pathogenicity of porcine reproductive and respiratory syndrome virus in Shandong area of China. Intl PRRS Symposium, Chicago, IL.

Yang X, Koziel JA, Cutler T, Zhang S, Zimmerman J, Hoff SJ, Jenks W, van Leeuwen J, Harmon J, Faulhaber C, Laor Y, Ravid U, Armon R. 2008. Treatment of livestock odor and pathogens with ultraviolet light. American Society of Agricultural and Biological Engineers (ASABE) Paper No. 085198. ASABE Annual International Meeting. Providence, Rhode Island.

Yoon K-J. 2008. PRRS diagnosis as tool for evidence-based PRRS control. KASV.
Zimmerman J, Prickett J, Johnson J. 2009. Oral fluid testing: Science-based applications. Carlos Pijoan International Symposium: New Approaches to Herd Diagnostics. 2009 Allen D. Leman Swine Conference. pp. 11-14.

Zimmerman J, Prickett J, Johnson JH, Molina R. 2009. Evaluando fluidos orales: Base científica de su aplicación. XVIII Día del Porcicultor 2009. Asociación de Médicos Veterinarios Zootecnistas Especialistas en Ciencias Porcicolas del Sur de Sonora A.C. Navojoa, Sonora, Mexico (CD).

Zimmerman J, Prickett J. 2008. Future epidemiologic / ecologic methods. 1st Annual Boehringer Ingelheim Swine Academy. Ames, Iowa, Vol 1, pp. 251-260.

Zimmerman J, Prickett JR, Molina R, Hoffmann P, Main R, Sornsen S, Johnson J. 2008. Vigilancia epidemiológica de enfermedades infecciosasa en poblaciones porcinis comerciales utilizando fluidos orales. Memorias XXI Congreso Panamericano de Ciencias Veterinarias. Guadalajara, México, pp. 231-234.

Zimmerman J. 2009. The Big Picture: Infectious disease, oral fluid testing, and swine health. 2nd Annual Boehringer Ingelheim Swine Academy. Ames, Iowa, pp. 181-200.

Xiuqing Wang, Hanmo Zhang, Xueshui Guo. 2009. The interaction between PRRSV and type I interferon induction signaling pathways. 28th American Society for Virology.

Yoo, D., Y. Sun, and N. Chen. 2008. One-step mutagenesis of the full-length infectious clone of PRRSV and generation of engineered viruses. Intl PRRS Symposium, Chicago, IL, Dec 6-7.

Zuckermann, F.A. , Calzada-Nova, G., Schnitzlein, W., Husmann, R. 2009. Proficient isolation and titration of field PRRS virus from clinical samples using a porcine alveolar macrophage cell line. 2009 Intl PRRS Symposium, Chicago, IL, Dec 6-7.



FUNDING SOURCES FOR PRRSV RESEARCH: Currently Funded Competitive Research Grants:

Blecha, et al, USDA NRI, 2006-2009. Porcine antimicrobial peptides and Toll-like receptors in PRRS pathogenesis, $340,000.

Christopher-Hennings J, Y Fang, J Lunney, EA Nelson. Development of a rapid, single tube, multiplex test to simultaneously detect immune parameters (cytokines) induced by PRRSV. National Pork Board, $101,107.

Christopher-Hennings J, Y Fang, EA Nelson. Elimination of PRRSV from semen: On Farm Mechanical and Antiviral Methods. National Pork Board $94,558, 2007-2009.

Enjuanes L. Induction of cross-protective immunity without exposure to live PRRSV (NPB #08-197). National Pork Board. 2008-2009.

Enjuanes L. Mechanisms inducing protection against coronaviruses and arteriviruses. Fort Dodge, S.A. 2008-2009.

Enjuanes L. Plant Production of Vaccines (PLAPROVA, EU 227056). European Communities. 2009-2011.

Faaberg KS, Collins JE, Yoon K-J, Christopher-Hennings J, Crow JA, Leung FC. 09/01/07-08/30/08. Implementation of a PRRSV strain database. National Pork Board PRRS Initiative. $51,322.

Faaberg, Guo, Miller: NPB, 11/01/09-11/01/10, $53,877, Molecular Identification of Type I Interferon Antagonistic Components of PRRSV Proteins.

Faaberg: NRICGP, 10/1/06-6/30/10, $274,998, Biological Studies of Putative Nonstructural Protein 2 in Porcine Reproductive and Respiratory Syndrome Virus.

Fang Y, JK Lunney, J Christopher-Hennings, E Nelson, A Young. The role of PRRSV non-structural proteins 1 and 2 in host immunity. USDA-NRI, $375,000, 1/08-12/2010.

Fang Y, W Zhang, J Christopher-Hennings, EA Nelson, RB Baker. Development of an epitope-based vaccine against swine influenza A using a non-toxic enterotoxin as the carrier-adjuvant. National Pork Board. $49,993.

Fang Y, Zimmerman J, Christopher-Hennings J, Nelson E, Murtaugh M, Lunney J. 01/01/10 to 12/31/11. Development of diagnostic assays for detecting PRRSV infection using oral fluid samples as an alternative to serum-based assays. National Pork Board - $99,989.

Gourapura RJ. Evaluation of adjuvants at the mucosal area for the development of innovative mucosal vaccine against PRRS. National Pork Board. Nov. 2008 to May 1, 2010.

Gourapura RJ. Study of mucosal immune responses in the respiratory tract of pigs infected with porcine reproductive and respiratory syndrome virus. OARDC Seed grant, The Ohio State University. March 2009 to February 2011.

Gourapura RJ. Development of novel mucosal vaccines for the control of PRRSV outbreaks. National Pork Board. Dec. 2009 to May, 2011.

Hesse and Rowland, 2009, Fort Dodge Animal Health, Heterotypic immunity as a platform for a new generation of modified live PRRS vaccines. $100,000

Holtkamp D, Ramirez A, O'Connor A, Zimmerman J. 01/01/09 to 12/31/09. Assessment of PRRS biosecurity in the field: Application of the American Assocation of Swine Veterinarians PRRS Risk Assessment Tool. USDA:CSREES National Research Initiative, Competitive Grants Program 230.1 Animal and Plant Biosecurity (subcontract: Kansas State University) - $24,300.

Holtkamp D, Zimmerman J. 10/01/08 to 09/30/09. Quantifying risk factors for PRRSV introduction into swine herds through the use of the PRRS Risk Assessment Tool. National Pork Board - $89,875.

Holtkamp D.J. A Cross-sectional Study Of PRRSV Positive Swine Breeding Herd Sites To Evaluate Associations Between Risk Factors And A Case Definition-based Number And Severity Of Clinical PRRS Episodes. Boehringer Ingelheim Vetmedica Inc. $115,000. Continuation of previous grant with same title. January 6, 2009.

Kim W-I, Yoon K-J, Cooper VC. 11/01/07-10/30/08. Identification of protective epitopes toward developing a vaccine providing broad cross-protection among PRRS viruses. National Pork Board PRRS Initiative. $89,150.

Kim W-I, Yoon K-J. 7/1/08-6/30/10. A new approach to PRRS vaccine that confers cross-protection against a broader range of PRRS viruses using chimeric mutant PRRS viruses. Iowa Healthy Livestock Initiative Research Grant. $39,980.

Laegreid W., F. Osorio, T. Goldberg, J. Christopher-Hennings, E. Nelson. Immunological consequences of PRRSV Diversity. USDA-NRI, PRRSV CAP2. $947,885.

Lunney J, J Christopher-Hennings, EA Nelson, Y Fang, JP Steibel, J Zimmerman. 01/01/10 to 12/31/11. Comparison of early immune responses of pigs which are genetically PRRS resistant/tolerant using a swine-specific immune protein (cytokine) multiplex assay. National Pork Board. $103,929.

Lunney JK, J Dekkers, R Fernando, Z Jiang, H-C Liu, R Pogranichniy, JM Reecy, R Rekaya, M Rothschild, D Smith, JP Steibel, C Tuggle. PRRS CAP Host genetics: Characterization of host factors that contribute to PRRS disease resistance and susceptibility. USDA NIFA PRRS CAP2: Objective 3 Host Genetics. $560,000. 2009-2012.

Lunney JK,C Ernst, V. Honavar, Z Jiang, R Pogranichniy, JP Steibel, C Tuggle. Identifying porcine genes and gene networks involved in effective response to PRRS virus using functional genomics and systems biology. USDA AFRI/NIFA Animal Genome, Genetics, and Breeding Program. $750,000. 2010-2012

Meng, X. J., Y. Fang, T. Opriessnig. Innovative approaches to develop a broadly protective and effective vaccine(s) against PRRSV. USDA PRRS CAP2, $100,000, 1/200912/2010.

Miller, Harhay, Lager: NPB, 11/01/08-11/01/10, $139,152, Gene Expression in lymph nodes of PRRSV-infected pigs

Murtaugh M (and MN stations). PRRS CAP 2, Minnesota Pork Board, National Pork Board, USDA, University of Minnesota Swine Disease Eradication Center, Minnesota Rapid Agricultural Response Fund.

Murtaugh M, Gourapura RJ. Positive Prognosticators of Immune Protection and Prophylaxis against PRRSV in Swine Herds. PRRSV PRRS CAP 2. August 2009 to July 2013.

Osorio FA: Immunologic Consequences of PRRSV Diversity, USDANRICGP CAP2 (Kansas State University subcontract), $74,368 August 2009-July 2010.

Osorio FA: Development of a modified live vaccine against PRRSV with optimal DIVA marker potential, National Pork Board, Grant Period: 11/01/2008 - 12/31/2009 (extended at no cost) $125,700

Osorio FA: Porcine Reproductive and Respiratory Virus: role of viral genes in virulence/attenuation, USDA NRICGP Project No. No.2008-00903, Period: 09/01/2008 - 08/31/2011, $374,900.

Pattnaik AK.; Molecular Structures of PRRSV that Contribute to PRRSV Protective Immunity. National Pork Board. $ 138,600; 12/01/2009-11/31/2010.

Pattnaik AK.; Glycoproteins of Porcine Reproductive and Respiratory Syndrome Virus in Infection and Immunity?; States Department of Agriculture, AFRI (2009-01576), $371,230; 09/01/2009-08/31/2012.

Pattnaik AK.: Role of All of PRRSV Glycoproteins in Protective Immune Response, 11/01/2008 - 10/31/2009 (extended at no cost) National Pork Board, $106,000.

Rowland RRR, Lunney JK, Reecy J, Johnson, R, NPB, 2007-2008. PRRS host genetics consortium: A proposal to develop a consortium to study the role of host genetics and resistance to PRRSV. $300,000.

Rowland RRR, Lunney JK, Reecy J, NPB, 2009-2010. PRRS host genetics consortium: A proposal to develop a consortium to study the role of host genetics and resistance to PRRSV. $247,000.

Rowland et al., USDA NRI Coordinated Agricultural Program (CAP), 2008-2012. Integrated strategies to control and reduce the impact of PRRS virus control, $4.8 million.

Wang, X. Interaction between PRRSV and interferon alpha/beta induction signaling pathways. USDA NRI 12/2008-11/2010 $ 100,000.

Yoo D. Private industry, $90,000, Antiviral effects of tilmicosin on swine respiratory viruses. 2008-2009.

Yoo D. USDA CSREES NRI, $375,000, Evasion strategies of PRRSV from the host defense. 2008-2011.

Yoon K-J. 1/1/07-12/31/10. Development of surveillance program and vaccine for PRRS in Korea: Development of a differential test for PRRS vaccine virus and immunological study of viral factors for protective immunity. Korean Ministry of Agriculture and Forestry (c/o National Veterinary Research and Quarantine Service). $280,000.

Zimmerman J, Dee SA, Davies PR, Holtkamp DJ, OConnor A. 08/01/09 to 07/31/10. Identifying ecologic and epidemiologic factors in the control of PRRS: A field-based approach. USDA:CSREES National Research Initiative, Competitive Grants Program 230.1 Animal and Plant Biosecurity (subcontract: Kansas State University) - $116,991.

Zimmerman J, Hoff SJ. 07/01/09 to 06/30/10. Effect of temperature and humidity on ultraviolet (UV) inactivation of airborne PRRS virus. Innovative Swine Industry Enhancement Grant Program, Iowa Attorney Generals Office - $43,046.

Zimmerman J. 05/01/09 to 04/31/10. PRRS CAP Graduate and Undergraduate Scholar Program Application - Iowa State University. USDA:CSREES National Research Initiative, Competitive Grants Program 230.1 Animal and Plant Biosecurity (subcontract: Kansas State University) - $23,999. Gold Sheet #97704.

Zuckermann F. USDA NRI CPG, $369,064. In vivo analysis of PRRS virus immunopathogenesis. Tracking Number: GRANT00168948. 2007-2010.

12/03/2010

See attachment under Summary of Minutes for complete NC229 Annual Report

12/02/2011

Abrahante JE, Martins K, Papas KK, Hering VJ, Schuurman H-J, Murtaugh MP. 2011. Microbiological safety of porcine islets: comparison with source pig. Xenotransplantation. 18:88-93.
Baker SA, Mondaca E, Polson D and Dee SA. Evaluation of a needle-free injection device (AcuShot) for reduction of hematogenous transmission of PRRS virus. Swine Health Prod (accepted for publication).
Baker SR, ONeil K, Gramer M and Dee SA. An estimate of seroprevalence of production-limiting diseases in wild swine. Vet Rec 2011;168:564
Beura LK, Dinh PX, Osorio FA, Pattnaik AK.(2011) Cellular Poly(C) Binding Proteins 1 and 2 Interact with Porcine Reproductive and Respiratory Syndrome Virus Non-Structural Protein 1{beta} and Support Viral Replication. J Virol. 2011 Oct 5. In press [Epub ahead of print]
Binjawadagi B, V. Dwivedi, C. Manickam, J.B. Torrelles, and G.J. Renukaradhya (2011) Intranasal delivery of an adjuvanted modified live porcine reproductive and respiratory syndrome virus vaccine reduces the ROS production. Viral Immunol., In press.
Bishop SC, Lunney JK, Pinard-van der Laan MH, Gay CG. 2011. Report from the Second International Symposium on Animal Genomics for Animal Health: Critical Needs, Challenges and Potential Solutions. Proc. Intnl. Symp. Animal Genomics for Animal Health (AGAH 2010). BMC Proceedings 5 Suppl 4:S1.
Boddicker, N, EH Waide, RRR Rowland, JK Lunney, DJ Garrick1, JM Reecy, JCM Dekkers. Major QTL associated with host response to porcine reproductive and respiratory syndrome virus challenge. J Anim Sci. In Revision.
Bradley, K.C., Jones, C.A., Tompkins, S.M., Tripp, R.A., Russell, R.J., Gramer, M.R., Heimburg-Molinaro, J., Smith, D.F., Cummings R.D., and D.A. Steinhauer. 2011 Comparison of the receptor binding properties of contemporary swine isolates and early human pandemic H1N1 isolates (novel 2009 H1N1). Virology. 413(2):169-82. PMID: 21353280
Brar MS, Shi M, Carman S, Ge L, Murtaugh MP, Leung FC-C. 2011. Porcine reproductive and respiratory syndrome virus in Ontario, Canada 1999-2010: diversity and restriction fragment length polymorphism. J Gen Virol. 92:1391-1397. PMID: 21346028.
Calzada-Nova, G., Schnitzlein, W., Husmann, R., Zuckermann, F.A. 2011. North American porcine reproductive and respiratory viruses suppress the type I interferon response of activated porcine plasmacytoid dendritic cells. J. Virol. 85:2703-13.
Chittick WA, Stensland WR, Prickett JR, Strait EL, Harmon K, Yoon K-J, Wang C, Zimmerman JJ. 2011. Comparison of RNA extraction and RT-PCR methods for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) in porcine oral fluid specimens. J Vet Diagn Invest 23:248-253.C
Cino-Ozuna, AG, S Henry, R Hesse, JC Nietfeld, J Bai, HM Scott, RRR Rowland.2011. Characterization of a new disease syndrome associated with porcine circovirus type 2 (PCV2) in previously vaccinated herds. J Clin Micro. In press.
Corzo C, Mondaca E, Wayne S, Torremorell M, Dee S, Davies P, Morrison B (2010). Control and elimination of porcine respiratory syndrome virus. Virus Res, 154:185-192, doi:10.1016/j.virusres.2010.08.016
Cutler T, Wang C, Qin Q, Zhou F, Warren K, Yoon K-J, Hoff SJ, Zimmerman J. 2011. Kinetics of UV254 inactivation of selected viral pathogens in a static system. J Appl Microbiol 111:389-395.
Cutler TD, Wang C, Hoff SJ, Kittawornrat A, Zimmerman JJ. 2011. Median infectious dose (ID50) of porcine reproductive and respiratory syndrome virus isolate MN-184 via aerosol exposure. Vet Microbiol 151:229-237.
Cutler TD, Zimmerman JJ. 2011. Ultraviolet irradiation and the mechanisms underlying its inactivation of infectious agents. Anim Health Res Rev 12:15-23.
Das PB, Vu HL, Dinh PX, Cooney JL, Kwon B, Osorio FA, Pattnaik AK.(2011)Glycosylation of minor envelope glycoproteins of porcine reproductive and respiratory syndrome virus in infectious virus recovery, receptor interaction, and immune response. Virology. 2011 Feb 20; 410(2):385-94. Epub 2010 Dec 30.
Debin Tian, Haihong Zheng, Rong Zhang, Jinshan Zhuang, Shishan Yuan. 2011. Chimeric Porcine Reproductive and Respiratory Syndrome Viruses reveal full function of genotype 1 envelope proteins in the backbone of genotype 2. Virology. YVIRO-06057; No. of page: 8; 4C: 4.
Dee S, Spronk G, Reicks D, Ruen P and Deen J. Further assessment of air filtration for preventing PRRSV infection in large breeding herds. Vet Rec 2010;167:976-977.
Dee SA, Otake S and Deen J. An evaluation of ultraviolet light (UV 254) as a means to inactivate porcine reproductive and respiratory syndrome virus on common farm surfaces and materials. Vet Microbiol 2011;150:96-99
Dee SA, Otake S and Deen J. Use of a production region model to assess the efficacy of various air filtration systems for preventing the airborne transmission of porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Results of a 2-year study. Virus Res 2010. 154:177-184.
Dee SA, Otake S, Pitkin A and Deen J. A 4-year summary of air filtration system efficacy for preventing airborne spread of porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Swine Health Prod 2011;19:292-294.
Dlugolenski, D., Jones, L., Saavedra, G., Tompkins. S.M., Tripp R.A., and E. Mundt. 2011 Passage of low-pathogenic avian influenza (LPAI) viruses mediates rapid genetic adaptation of a wild-bird isolate in poultry. Arch Virol. 156(4):565-76. PMID: 21197555
Driskell, E.A., Jones, C.A., Berghaus, R.D., Stallknecht, D.E., Howerth, E.W., and S.M. Tompkins. 2011 Domestic cats are susceptible to infection with low pathogenic avian influenza viruses from shorebirds. In press, Veterinary Pathology.
Driskell, E.A., Jones, C.A., Stallknecht, D.E., Howerth, E.W., and S.M. Tompkins. 2010 Avian influenza isolates from wild birds replicate and cause disease in a mouse model of infection. Virology. 399(2):280-9. PMID: 20123144
Driskell, J.D., Jones, C.A., Tompkins, S.M., and R.A. Tripp. 2011 One-Step Assay for Detecting Influenza Using Dynamic Light Scattering and Gold Nanoparticles. Analyst 136(15):3083-90. PMID: 21666913 Cover Article.
Du, Y., D. Yoo, M. A. Paradis, and G.Scherba. 2011. Antiviral activity of tilmicosin for type 1 and 2 porcine reproductive and respiratory syndrome virus in cultured porcine alveolar macrophages. J. Antivirals Antiretrovirals 3:28-33.
Dwivedi V, C. Manickam, R. Patterson, K. Dodson, and G.J. Renukaradhya (2011). Intranasal delivery of whole cell lysate of Mycobacterium tuberculosis induces protective immune responses to a modified live porcine reproductive and respiratory syndrome virus vaccine in pigs. Vaccine, 29(23):4067-76.
Dwivedi V, Manickam C, Patterson R, Dodson K, Murtaugh M, Torrelles JB, Schlesinger LS, Renukaradhya GJ. 2011. Cross-protective immunity to porcine reproductive and respiratory syndrome virus by intranasal delivery of a live virus vaccine with a potent adjuvant. Vaccine 29:4058-4066.PMID: 21419162
Faaberg, K. S., Kehrli, Jr., M. E., Lager, K. M., Guo, B., and J. Han. 2010. In vivo growth of porcine reproductive and respiratory syndrome virus engineered nsp2 deletion mutants. Virus Res. 154:77-85.
Fei Gao, Jiaqi Lu, Huochun Yao, Zuzhang Wei, Qian Yang, Shishan Yuan, Cis- Acting Structural Element in 5 UTR Is Essential for Replication of Porcine Reproductive and Respiratory Syndrome Virus. Virus Research, [J] 2011. 10.1016/j.virusres.2011.08.018.
Feifei Tan, Zuzhang Wei, Yanhua Li, Rong Zhang, Jinshan Zhuang, Zhi Sun, Shishan Yuan. 2011. Identification of non-essential regions in nucleocapsid protein of Porcine and Respiratory Syndrome Virus for replication in cell culture. Virus Research. Volume 158, issues 1-2, June 2011, page 62-71.
Gauger PC, Faaberg KS, Guo B, Kappes MA, Opriessnig T. 2011. Genetic and phenotypic characterization of a 2006 United States porcine reproductive and respiratory virus isolate associated with high morbidity and mortality in the field. Virus Res [Epub ahead of print]
Gauger PC, Lager KM, Vincent AL, Opriessnig T, Cheung AK, Butler JE, Kehrli ME, Jr (2011) Leukogram abnormalities in gnotobiotic pigs infected with porcine circovirus type 2. Veterinary Microbiology 154:185-190
Gauger PC, Lager KM, Vincent AL, Opriessnig T, Kehrli ME, Jr, Cheung AK (2011) Postweaning multisystemic wasting syndrome produced in gnotobiotic pigs following exposure to various amounts of porcine circovirus type 2a or type 2b. Veterinary Microbiology 153:229-239
Gauger PC, Vincent AL, Loving CL, Lager KM, Janke BH, Kehrli Jr. M.E, Roth JA. 2011. Enhanced pneumonia and disease in pigs vaccinated with an inactivated human-like (´-cluster) H1N2 vaccine and challenged with pandemic 2009 H1N1 influenza virus. Vaccine 24:2712-2719.
Gordy, J.T., Jones,C.A., Rue, J., Crawford, P.C., Levy, J.K., Stallknecht, D.E., Tripp, R.A., and S.M. Tompkins. 2011 Surveillance of Feral Cats for Influenza A Virus. In Press, Influenza and Other Respiratory Pathogens.
Gorres JP, Lager KM, Kong W-P, Royals M, Todd JP, Vincent AL, Wei C-J, Loving CL, Zanella EL, Janke B, Marcus E. Kehrli J, Nabel GJ, Rao SS (2011) DNA Vaccination Elicits Protective Immune Responses against Pandemic and Classic Swine Influenza Viruses in Pigs. Clinical and Vaccine Immunology 18:1987-1995
Guo, B., Vorwald, A.C., Alt, D.P., Lager, K.M., Bayles, D.O. and Faaberg, K.S. 2011. Large scale parallel pyrosequencing technology: PRRSV strain VR-2332 nsp2 deletion mutant stability in swine. Virus Res. 161:162-169
Haley C, Wagner B, Puvanendiran S, Abrahante J, Murtaugh MP. 2011. Diagnostic performance measures of ELISA and quantitative PCR tests for porcine circovirus type 2 exposure using Bayesian latent class analysis. Prev Vet Med. 101:79-88. PMID: 21632130
Hamir AN, Greenlee JJ, Stanton T, Smith JD, Doucette S, Kunkle RA, Stasko JA, Richt JA, Kehrli ME, Jr (2011) Experimental inoculation of raccoons (Procyon lotor) with Spiroplasma mirum and transmissible mink encephalopathy (TME) Canadian Journal of Veterinary Research 75:18-24
Hamir AN, Kehrli ME, Jr, Kunkle RA, Greenlee JJ, Nicholson EM, Richt JA, Miller JM, Cutlip RC (2011) Experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle: comparison to bovine spongiform encephalopathy in cattle. J Vet Diagn Invest 23:407-420
He D, C. Overend, J. Ambrogio, R.J. Maganti, M.J Grubman, A.E Garmendia 2011. Marked differences between MARC-145 cells and swine alveolar macrophages in IFN²-induced activation of antiviral state against PRRSV. Vet Immunol Immunopathol 139(1):57-60.
Hiep, L. X. Vu., Byungjoon, K., Kyoung-Jin Y., LAegreid, W., Pattnaik, A.K., Osorio, F.A. 2011. Immune Evasion of Porcine Reproductive and Respiratory Syndrome Virus through Glycan Shielding Involves both Glycoprotein 5 as Well as Glycoprotein 3. J. Virol. 85:5555-64.
Holtkamp DJ, Polson DD, Torremorell M (2011). Terminology for classifying swine herds by porcine reproductive and respiratory syndrome virus status. J Swine Health and Prod 19(1):44-56.
Holtkamp DJ, Polson DD, Torremorell M (2011). Terminology for classifying swine herds by porcine reproductive and respiratory syndrome virus status. Tierärztliche Praxis Großtiere 39 2: 101-112
Huang YW, Meng XJ. Novel strategies and approaches to develop the next generation of vaccines against porcine reproductive and respiratory syndrome virus (PRRSV). Virus Research. 2010;154(1-2):141-9.
Irwin CK, Yoon K-J, Wang C, Hoff SJ, Zimmerman JJ, Denagamage T, O'Connor AM. 2011. Using the systematic review methodology to evaluate factors that influence the persistence of influenza in environmental matrices. Appl Environ Microbiol 77:1049-1060.
Jiaqi Lu, Fei Gao, Zuzhang Wei, Ping Liu, Changlong Liu, Haihong Zheng, Yanhua Li, Tao Lin, Shishan Yuan. 2011. A 5-Proximal Stem-loop Structure of 5 Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication. Virology Journal 2011, 8: 172, doi: 10. 1186/ 1743-422X-8-172.
Johnson CR, Griggs TF, Gnanandarajah J, Murtaugh MP. 2011. Novel structural protein in Porcine reproductive and respiratory syndrome virus encoded in an alternative open reading frame 5 present in all arteriviruses. J Gen Virol 92:1107-1116. PMID: 21307222
Kappes MA, Sandbulte MR, Platt R, Wang C, Lager KM, Henningson JN, Lorusso A, Vincent AL, Loving CL, Roth JA, Kehrli ME, Jr. (2011) Vaccination with NS1-truncated H3N2 swine influenza virus primes T cells and confers cross-protection against an H1N1 heterosubtypic challenge in pigs. Vaccine (e-pub ahead of print 07Nov2011)
Kittawornrat A, Prickett J, Wang C, Panyasing Y, Ballagi A, Rice A, Main R, Johnson J, Rademacher C, Hoogland M, Rowland R, Zimmerman J. Detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in oral fluid specimens using a commercial PRRSV serum antibody ELISA. J Vet Diagn Invest (in press).
Langenhorst Robert J., Steven Lawson, Apisit Kittawornrat, Jeffrey J. Zimmerman, Zhi Sun, Yanhua Li, Jane Christopher-Hennings, Eric A. Nelson, Ying Fang. Development of a fluorescent microsphere immunoassay for detection of antibodies against PRRSV using oral fluid samples as an alternative to serum-based assays. Journal of Clinical Vaccine and Immunology.
Lawson, Steve, Yanhua Li, John Patton, Robert J. Langenhorst, Zhi Sun, Zhiyong Jiang, Jane Christopher-Hennings, Eric A. Nelson, David Knudsen, Ying Fang, Kyeong-Ok Chang. Interleukin-1² expression by a recombinant porcine reproductive and respiratory syndrome virus. Virus Research, in preparation.
Lin K, Wang C, Murtaugh MP, Ramamoorthy S. 2011. Multiplex method for simultaneous serological detection of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. J Clin Micro 49:3184-3190.
Linhares D, Rovira A, Torremorell M (2011). Evaluation of FTA cards for collection and transport of samples for PRRS virus diagnostics. Accepted for publication
Linhares D, Torremorell M, Cano JP and Dee SA. Effect of modified live porcine reproductive and respiratory syndrome (PRRS) vaccine on the shedding of wild-type virus from an endemically infected population of growing pigs. Vaccine (accepted for publication).
Lorusso A, Vincent AL, Harland ML, Alt D, Bayles DO, Swenson SL, Gramer MR, Russell CA, Smith DJ, Lager KM, Lewis NS (2011) Genetic and antigenic characterization of H1 influenza viruses from United States swine from 2008. The Journal of general virology 92:919-930
Lunney JK, DA Benfield DA, RR Rowland. 2010. Porcine reproductive and respiratory syndrome virus: an update on an emerging and re-emerging viral disease of swine. Virus Res. 154:1-6.
Lunney JK, Rowland RRR. 2011. Understanding Genetic Disease Resistance. National Hog Farmer. Blueprint Immunology 101. Apr. 15, 2011. p.30-42.
Lunney JK, Steibel JP, Reecy J, Rothschild M, Kerrigan M, Trible B, Rowland RRR. 2011. Probing genetic control of swine responses to PRRSV infection: Current Progress of the PRRS Host Genetics Consortium. Proceedings of the International Symposium on Animal Genomics for Animal Health (AGAH 2010). BMC Proceedings. 5 Suppl 4:S30.
Nfon CK, Berhane Y, Hisanaga T, Zhang S, Handel K, Kehler H, Labrecque O, Lewis NS, Vincent AL, Copps J, Alexandersen S, Pasick J (2011) Characterization of H1N1 swine influenza viruses circulating in Canadian pigs in 2009. Journal of virology 85:8667-8679
Ni YY, Huang YW, Cao D, Opriessnig T, Meng XJ. Establishment of a DNA-launched infectious clone for a highly pneumovirulent strain of type 2 porcine reproductive and respiratory syndrome virus: identification and in vitro and in vivo characterization of a large spontaneous deletion in the nsp2 region. Virus Research. 2011;160 (1-2):264-73.
Nicholson, T. L., D. Kukielka, A. L. Vincent, S. L. Brockmeier, Miller, L. C. and K. S. Faaberg. 2011. Utility of a Panviral Microarray for Detection of Swine Respiratory Viruses in Clinical Samples. J. Clin. Microbiol. 49:1542-1548.
ONeill K, Shen HG, Lin K, Hemann M, Beach NM, Meng XJ, Halbur PG, Opriessnig T. 2011. Studies on PCV2 vaccination of 5-day-old piglets. Clin Vaccine Immunol [Epub ahead of print]
Opriessnig T, Gomes-Neto J, Hemann M, Shen HG, Huang YW, Halbur PG, Meng XJ. 2011. An experimental live chimeric PCV1-2a vaccine is efficacious at decreasing PCV2b viremia when administered intramuscularly or orally in a PCV2b and PRRSV dual-challenge model. Microbiol Immunol [Epub ahead of print]
Opriessnig T, Madson DM, Roof M, Layton S, Ramamoorthy S, Meng XJ, Halbur PG. 2011. Experimental reproduction of porcine circovirus type 2 (PCV2)-associated enteritis in pigs infected with PCV2 alone or concurrently with Lawsonia intracellularis or Salmonella typhimurium. J Comp Pathol (in press).
Opriessnig T, Madson DM, Schalk S, Brockmeier S, Shen HG, Beach NM, Meng XJ, Baker, RB, Zanella EL, Halbur PG. 2011. Porcine circovirus type 2 (PCV2) vaccination is effective in reducing disease and PCV2 shedding in semen of boars concurrently infected with PCV2 and Mycoplasma hyopneumoniae. Theriogenology 76:351-360.
Opriessnig T, Patel D, Halbur PG, Meng XJ, Stein DA, Zhang YJ. 2011. Inhibition of porcine reproductive and respiratory syndrome virus infection in piglets by a peptide-conjugated morpholino oligomer. Antiviral Res 91:36-42.
Opriessnig T, Patterson AR, Madson DM, Pal N, Ramamoorthy S, Meng XJ, Halbur PG: 2010. Comparison of the effectiveness of passive (dam) versus active (piglet) immunization against porcine circovirus type 2 (PCV2) and impact of passively-derived PCV2 vaccine-induced immunity on vaccination. Vet Microbiol 142:177-183.
Opriessnig T, Shen H-G, Pal N, Ramamoorthy S, Huang Y-W, Lager KM, Halbur PG, Meng X-J. 2011. A live-attenuated chimeric vaccine based on the new porcine circovirus subtype 2b (PCV2b) is safe and efficacious in a triple challenge co-infection model with PCV2b, porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus. (in press).
Opriessnig T, Shen HG, Pal N, Ramamoorthy S, Huang YW, Lager KM, Beach NM, Halbur PG, Meng XJ. 2011. A live-attenuated chimeric porcine circovirus Type 2 (PCV2) vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) and is efficacious in a PCV2b-PRRSV-PPV challenge model. Clin Vaccine Immunol 18:1261-1268.
Opriessnig, T., Shen, H.G., Pal, N., Ramamoorthy, S., Huang, Y.W., Lager, K.M., Beach, N.M., Halbur, P.G., and Meng, X.J., A live-attenuated chimeric PCV2 vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with PPV and PRRSV and is efficacious in a PCV2a-PRRSV-PPV challenge model. Clinical and Vaccine Immunology. 29(15):P.2712-9
Opriessnig, T., Shen, H.G., Pal, N., Ramamoorthy, S., Huang, Y.W., Lager, K.M., Beach, N.M., Halbur, P.G., and Meng, X.J. 2011. A Live-Attenuated Chimeric Porcine Circovirus Type 2 (PCV2) Vaccine Is Transmitted to Contact Pigs but Is Not Upregulated by Concurrent Infection with Porcine Parvovirus (PPV) and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Is Efficacious in a PCV2b-PRRSV-PPV Challenge Model. Clin Vaccine Immunol 18, 1261-1268. PMID: 21653745
Oshansky, C.M., Pickens, J.A., Bradley, K.C., Jones, L.P., Saavedra-Ebner, G.M., Barber, J.P., Crabtree, J.M., Steinhauer, D.A., Tompkins, S.M., and R.A. Tripp. 2011 Avian influenza viruses infect primary human bronchial epithelial cells unconstrained by sialic acid ±2, 3 residues. PLoS ONE, 6(6):e21183. PMID: 21731666
Otake S, Dee S, Corzo C, Oliveira S and Deen J. Long distance airborne transport of viable PRRSV and Mycoplasma hyopneumoniae from a swine population infected with multiple viral variants. Vet Microbiol 2010; 145: 198-208.
Patterson AR, Baker RB, Madson DM, Pintar AL, Opriessnig T. 2011. Disinfection protocols reduce the amount of porcine circovirus type 2 contaminated livestock transport vehicles. J Swine Health Prod 19:156-164.
Patterson AR, Johnson JK, Ramamoorthy S, Hesse RA, Murtaugh MP, Puvanendiran S, Pogranichniy RM, Erickson GA, Carman S, Hause B, Meng XJ, Opriessnig T. 2011. Interlaboratory comparison of Porcine circovirus-2 indirect immunofluorescent antibody test and enzyme-linked immunosorbent assay results on experimentally infected pigs. J Vet Diagn Invest. 23:206-212..
Patterson AR, Madson DM, Schalk SD, Halbur PG, Opriessnig T. 2011. Establishment and maintenance of a porcine circovirus type 2 (PCV2)-free breeding herd on a site that experienced a natural outbreak of PCV2-associated reproductive disease. J Swine Health Prod 19:165-174.
Patterson AR, Ramamoorthy S, Madson DM, Meng XJ, Halbur PG, Opriessnig T. 2011. Shedding and infection dynamics of porcine circovirus type 2 (PCV2) after experimental infection. Vet Microbiol 149:91-98.
Pena L, Vincent AL, Ye J, Ciacci-Zanella JR, Angel M, Lorusso A, Gauger PC, Janke BH, Loving CL, Perez DR (2011) Modifications in the polymerase genes of a swine-like triple-reassortant influenza virus to generate live attenuated vaccines against 2009 pandemic H1N1 viruses. Journal of virology 85:456-469
Pitkin A, Otake S and Dee SA. A one-night downtime period prevents the spread of PRRSV and Mycoplasma hyopneumoniae by personnel and fomites (boots and coveralls)". Swine Health Prod (accepted for publication).
Platt R, Vincent AL, Gauger PC, Loving CL, Zanella EL, Lager KM, Kehrli ME, Jr., Kimura K, Roth JA (2011) Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs. Veterinary Immunology and Immunopathology 142:252-257
Prickett JR, Johnson J, Murtaugh MP, Puvanendiran S, Wang C, Zimmerman JJ, Opriessnig T. 2011. Prolonged detection of PCV2 and anti-PCV2 antibody in oral fluids following experimental inoculation. Transbound Emerg Dis. 58:121-127. PMID: 21223532
Puvanendiran S, Stone S, Yu W, Johnson CR, Abrahante J, Garcia Jimenez L, Griggs T, Haley C, Wagner B, Murtaugh MP. 2011. High prevalence of porcine circovirus type 2 exposure and infection in swine finisher herds. Virus Res. 157:92-98. PMID:21352865
Ramirez A, Wang C, Prickett JR, Pogranichniy R, Yoon K-J, Main R, Rademacher C, Hoogland M, Hoffmann P, Johnson JK, Kurtz A, Kurtz E, Zimmerman J. 2011. Efficient surveillance of pig populations using oral fluids. Prev Vet Med (in press).
Romagosa A, Gramer M, Joo HS, Torremorell M (2011). Sensitivity of oral fluids for detecting influenza A virus in populations of vaccinated and non-vaccinated pigs. J of influenza and other respiratory viruses. DOI:10.1111/j.1750-2659.2011.00276.x
Rowland, RRR. 2010. The interaction between PRRSV and the late gestation pig fetus . Virus Res., 154:114-122.
Shen H, Wang C, Madson DM, Opriessnig T. 2010. High prevalence of porcine circovirus viremia in newborn piglets in five clinically normal swine breeding herds in North America. Prev Vet Med 97:228236.
Shen HG, Beach NM, Huang YW, Halbur PG, Meng XJ, Opriessnig T. 2011. Comparison of commercial and experimental porcine circovirus type 2 (PCV2) vaccines using a triple challenge with PCV2, porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus (PPV). Vaccine 28:5960-5966.
Shen HG, Schalk S, Halbur PG, Campbell J, Russell L, Opriessnig T. 2011. Commercially produced spray dried porcine plasma contains high levels of PCV2 DNA but did not transmit PCV2 when fed to naïve pigs. J Anim Science 89:1930-1938.
Shi, M., T. T. Lam, C. C. Hon, R. K. Hui, K. S. Faaberg, T. Wennblom, M. P. Murtaugh, T. Stadejek, and F. C. Leung. 2010. Molecular epidemiology of PRRSV: A phylogenetic perspective. Virus Res. 154:7-17.
Sinha A, Schalk S, Lager K M, Wang C, Opriessnig T. 2011. Singular PCV2a or PCV2b infection results in apoptosis of hepatocytes 3 in clinically affected gnotobiotic pigs. Res Vet Sci DOI:10.1016/j.rvsc.2010.10.013
Sinha A, Shen HG, Schalk S, Beach NM, Huang YW, Halbur PG, Meng XJ, Opriessnig T. 2010. Porcine reproductive and respiratory syndrome virus (PRRSV) infection at the time of porcine circovirus type 2 (PCV2) vaccination has no impact on vaccine efficacy. Clin Vaccine Immunol 17:19040-1945.
Sinha A, Shen HG, Schalk S, Beach NM, Huang YW, Meng XJ, Halbur PG, Opriessnig T. 2011. Porcine reproductive and respiratory syndrome virus (PRRSV) influences infection dynamics of porcine circovirus type 2 (PCV2) subtypes PCV2a and PCV2b by prolonging PCV2 viremia and shedding. Vet Microbiol 152:235-246.
Smith, J.H., Brooks, P., Johnson, S., Tompkins, S.M., Custer, K.M., Haas, D.L., Mair, R., Papania, M., and R.A. Tripp. 2010 Aerosol Vaccination Induces Robust Protective Immunity to Homologous and Heterologous Influenza Infection in Mice. Vaccine 29(14): 2568-75. PMID: 21300100
Smith, J.H., Nagy, T., Driskell, E.A., Brooks, P., Tompkins, S.M., and R.A. Tripp. 2011 Comparative Pathology in Ferrets Infected with H1N1 Influenza A Viruses Isolated from Different Hosts. J Virol. 85(15):7572-81. PMID: 21593156
Smith, J.H., Papania, M., Knaus, D., Brooks, P., Hass, D.L., Mair, R., Barry, J., Tompkins, S.M., and R.A. Tripp. Nebulized Live-Attenuated Influenza Vaccine Provides Protection in Ferrets at a Reduced Dose. In Press, Vaccine.
Spronk G, Otake S and Dee S. Prevention of PRRSV infection in large breeding herds using air filtration. Vet Rec 2010; 166:758-759.
Subramaniam S, Sur JH, Kwon B, Pattnaik AK, Osorio FA.(2011) A virulent strain of porcine reproductive and respiratory syndrome virus does not up-regulate interleukin-10 levels in vitro or in vivo.Virus Res. 2011 Feb;155(2):415-22. Epub 2010 Dec 17.
Tiawsirisup S, Blitvich BJ, Tucker BJ, Halbur PG, Bartholomay LC, Rowley WA, Platt KB. 2010. Susceptibility of fox squirrels (Sciurus niger) to West Nile virus by oral exposure. Vector Borne Zoonotic Dis 10:207-209.
Trible, BR, M Kerrigan, N Crossland, M Potter, K Faaberg, R Hesse, RRR Rowland. Porcine circovirus type 2 capsid protein epitopes associated with vaccination, infection and disease. Clin Vacc Immunol. In press.
Trible, Kerrigan, M., Faaberg, K. S., and R. R.R. Rowland. 2011. Identification of an immunodominant region the PCV2 capsid protein recognized by naturally infected and vaccinated pigs. Clinical and Vaccine Immunology, 18:749-757.
Vincent, A. L., K. M. Lager, K. S. Faaberg, M. Harland, E. L. Zanella, J. R. Ciacci-Zanella, M. E. Kehrli, Jr., B. H. Janke, and A. Klimov. 2010. Experimental inoculation of pigs with pandemic H1N1 2009 virus and HI cross-reactivity with contemporary swine influenza virus antisera. Influenza Other Respiratory Viruses. 4:53-60.
Vu HL, Kwon B, Yoon KJ, Laegreid WW, Pattnaik AK, Osorio FA. (2011) Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3. J Virol. 2011 Jun;85(11):5555-64. Epub 2011 Mar 16.
Wagner J, Kneucker A, Liebler-Tenorio E, Fachinger V, Glaser M, Pesch S, Murtaugh MP, Reinhold P. 2011. Respiratory function and pulmonary lesions in pigs infected with porcine reproductive and respiratory syndrome virus. Vet. J. 187:310-319. PMID: 20089425
Zhang H, Lunney JK, Baker RB, Opriessnig T. 2011. Cytokine and chemokine mRNA expression profiles in tracheobronchial lymph nodes from pigs singularly infected or coinfected with porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae (MHYO). Vet Immunol Immunopathol 140:152-158.
Zhang H, Mohn U, Prickett JR, Schalk S, Motz M, Halbur PG, Feagins AR, Meng X-J, Opriessnig T. 2011. Different capabilities of enzyme immunoassays to detect anti-hepatitis E virus immunoglobin G in sera from pigs infected experimentally with hepatitis E genotype 3 or 4 and in sera from field pigs with unknown status of HEV infection. J Virol Methods (in press).

12/02/2012

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12/08/2013

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