S1060: Fly Management in Animal Agriculture Systems and Impacts on Animal Health and Food Safety
(Multistate Research Project)
S1060: Fly Management in Animal Agriculture Systems and Impacts on Animal Health and Food Safety
Duration: 10/01/2013 to 09/30/2018
Statement of Issues and Justification
Federal funding priorities are focused on major issues of national concern, climate change, food safety, food security, biofuels, and obesity. Entomologists play a key and vital role in helping to solve many of these national concerns by evaluating the potential impact of climate change on insect populations and how these changes can threaten the health and well being of humans and animals, and compromise the nations safe and secure food supply. Few insects are more influenced by anthropogenic effects than nuisance and pest flies; the house fly, stable fly, horn fly, face fly and blow flies. There is a significant body of literature on the biology and economic impact of these pests but this multidisciplinary project examines closely predictive models influencing pest distribution in light of climate change, the effects of the microbial community of pest populations, and the dispersal of pathogenic microorganisms that compromise a safe and secure food supply. Advances developed in the course of this project will lead to the development of new and innovative pest management technologies to mitigate these threats.
Biting and nuisance flies are among the most important pests in livestock and poultry production systems. These flies are responsible for damage and control costs in excess of a billion dollars per year in the United States (e.g., see Taylor et al. 2012). In addition to the direct damage these flies inflict upon livestock, their presence as a byproduct of confined livestock and poultry operations has been repeatedly cited as a nuisance, especially when flies enter the vicinity of human habitations and urban environments. Law suits, zoning limitations and animosity between farmers and home owners have resulted (Thomas and Skoda 1993). In spite of their ubiquitous presence, importance as pests, and association with diseases of humans and livestock, our knowledge of the biology of these species is seriously wanting and available control technologies remain inadequate. The recent sequencing of the house fly genome (underway), and future sequencing of the stable fly genome (planned) offer great potential for the identification of new opportunities for managing these pests.
House flies are considered to be the #1 nuisance pest associated with dairy and other confined animal operations (Geden and Hogsette 1994, Hinkle and Hickle 1999). House flies are capable of carrying more than 65 disease organisms that affect humans and animals (Greenberg 1971), such as the virulent Escherichia coli strain O157:H7 (Sasaki et al. 2000). In poultry production, house flies can transmit Salmonella among flocks; and the spotting of eggs with fly specks may reduce the eggs market value. Stable are among the most serious pests of cattle worldwide. With their painful bites, they can reduce weight gains of cattle on finishing rations up to 20% (Campbell et al. 1977). The total impact to U.S. cattle industries is estimated to exceed $2 billion dollars annually (Taylor et al. 2012). Given the economic importance of nuisance and biting flies, control of their populations is critically important. For decades insecticides have provided economical control of these pests. However, the evolution of insecticide resistance compromises the control achieved in many locations around the USA.
Stable flies develop as maggots in a wide array of decomposing organic matter, including soiled animal bedding and soiled feed debris that accumulates wherever cattle are confined (Moon, 2002). Populations build exponentially by continuous reproduction from spring to fall in northern temperate localities (Beresford and Sutcliffe, 2010; Taylor et al., 2012). Dairy farm surveys indicate calf hutch bedding is a prominent source of stable flies around dairies (Schmidtmann, 1988), and choice of bedding material can minimize stable fly production (Schmidtmann, 1991). More recently, it has also become apparent that feed debris and manure that accumulate during winter are important sources of stable flies, especially where overwintered debris piles remain intact into the following summer (Broce et al., 2005; Talley et al., 2009; Taylor and Berkebile, 2011).
The face fly is the primary pest of pastured cattle in most state north of the 35th parallel. Adult face flies overwinter in attics and out-buildings and colonize cattle in the spring (Karfsur and Moon 1997). The face fly feeds on lachrymal and mucosal secretions of the eyes and nose of cattle. Gravid flies lay eggs exclusively in fresh cattle dung pats, and the life cycle can be completed in as little as 14 days. When face flies are abundant, cattle change grazing habits, which often results in poor utilization of pasture. In addition to the annoyance and irritation associated with its feeding habits, the face fly is the primary means of transmission of Moraxella bovis, the causative agent of infectious bovine keratoconjunctivitis (IBK), also known as pinkeye (Glass et al. 1982, Glass and Gerhardt 1983, Krasfur and Moon 1997). Face fly infestations were estimated to cause annual losses of more than $53 million (Drummond et al. 1981). Action threshold levels of 10-15 flies per face were established to reduce the spread of pinkeye and maximize animal comfort (Krafsur & Moon 1997). In the northeast face fly numbers often exceed 100 flies per face.
The horn fly is an obligate blood-sucking parasite of cattle and is considered a serious pest of pastured cattle in US (Drummond 1988). Horn fly feeding annoys cattle, alters their grazing habits, and decreases both milk production and weight gains. Horn fly numbers as high as 10,000 per animal have been reported and they feed 10 to 12 times per day. Horn flies oviposit exclusively in fresh dung, and they do so immediately after it has been deposited (Bruce 1964). The fly can complete development in 9-12 days, with 50% adult survival at 5 weeks. Horn flies diapause beneath dung pats during the winter months. Horn fly control leads to increased milk production and calf growth (Johnsson and Mayer, 1999). Unlike other kinds of flies that just visit cattle for brief moments, adult horn flies reside on their host animals, which makes then especially vulnerable to control. Organic dairy farmers rely on essential oil repellents to alleviate horn fly problems, but success of these products is limited. Horn flies have been incriminated in the transmission of bovine mastitis, also known as summer mastitis (Oliver et al. 1998, Gillespie et al. 1999, Edwards et al. 2000). In NC, 53% of horn flies collected from cattle were positive for S. aureus, and 39% of the cows were positive for the same genotype found in the flies (Anderson et al. 2012).
In 2003, the Northeastern IPM Center Livestock and Field Crop working group created a list of prioritized needs (http://northeastipm.org/work_livepriority.cfm). The group indicated that the development of new integrated management of key pests of livestock and poultry in confined and pasture settings was a top priority with specific reference to stable fly breeding and migration in pasture systems and fly control methods for pasture and feedlot situations. Ten of the working groups 17 assessed needs and seven of the top 10 directly referred to muscid flies, including house flies, stable flies, and face flies as top priorities. The objectives of the current proposal address 10 of the 17 needs. Coordinated extension of the research outcomes derived from this proposal to stakeholders will address 2 additional priorities of this working group.
In 2001, research and extension needs for IPM of arthropods of veterinary importance that were identified as part of a USDA sponsored workshop in Lincoln, Nebraska nearly 20 years ago (Geden and Hogsette 1994) were reevaluated, updated, and the updated document is now available at: http://www.ars.usda.gov/Services/docs.htm?docid=10139 . This document describes the IPM needs of eight animal commodity groups including poultry, dairy, beef cattle, and swine. For each of these commodity groups, muscid flies are noted as a very significant pest, and the working group makes strong recommendations for increased research and extension efforts to reduce the considerable economic losses resulting from pest activities. This workgroup also noted the decline to critical levels of extension personnel nationally, particularly related to domestic animal production. Increasing coordination and collaboration among veterinary entomologists nationally is needed to more efficiently disseminate research findings and management recommendations.
Successful completion of this project will provide a better understanding of the interactions between livestock production systems and the life cycles of pestiferous flies. Exploitation of these interactions will provide economically feasible and environmentally friendly technology for reducing the impact of flies on livestock production and human health. The project will provide quantitative data to analyze fly borne spread of pathogens from animal production systems into the urban environment. The project will develop new control technologies for biting and nuisance flies and will assess the fly resistance to insecticides that are currently available or under development. New technological innovations and comprehensive pest management information will be disseminated to producers through a multistate coordinated effort to provide the broadest reach for project outcomes thereby increasing the health and quality of livestock and reducing the economic impact of these pest flies.
The expertise to accomplish the objectives of this project exists within the university and USDA-ARS systems. However, expertise is widely dispersed with few states having more than one livestock entomologist and many having none. A Multistate Project will serve to coordinate this research effort, maximizing synergy and minimizing duplicated effort.
This project will replace the existing S-1030 project Flies impacting livestock, poultry and food safety. Most of the recent work cited below has been the result of previous projects in this series.
Related, Current and Previous Work
See attachment for complete Related, Current and Previous Work section due to character limit
New technologies for management of biting and nuisance flies in organic and conventional systems
Insecticide resistance detection and management
Investigation of the microbial ecology, epithelial immunity, and vector competence of biting and nuisance flies
Characterize population biology of biting and nuisance flies
Community and stakeholder engagement
Methods1. New technologies for management of biting and nuisance flies in organic and conventional systems a. Novel push-pull strategies (NE, NC, CA) b. Evaluation of improved monitoring systems (AR, NE, CA) c. Novel toxicants and delivery systems (TX, FL, NE) d. Non-pesticide management options (biological, cultural and mechanical) (FL, WA, CA, MN, TN, AR, NC, USDA) 2. Insecticide resistance detection and management a. Assessment of insecticide resistance (NY, FL, USDA) b. Leveraging the Stomoxys and Musca genomes for novel control measures (NY, USDA) 3. Investigation of the microbial ecology, epithelial immunity, and vector competence of biting and nuisance flies a. Identification of the key bacterial strains and their metabolites playing a major role in oviposition and larval development of stable flies (TX, OK, KS, USDA) b. Investigation of the midgut epithelial immunity of house flies, stable flies, and biting midges (KS, USDA) c. Animal and human pathogen acquisition, dispersal and deposition by house flies (NC, OK, CA, MD, KS) 4. Characterize population biology of biting and nuisance flies a. Characterize effects of climate and landscape features on dispersal (FL, OK, NE, NC, USDA, Canada) b. Phenological and environmental effects on biting and nuisance fly populations (MN, NE, FL, USDA, Canada) c. Larval developmental habitat source identification (NE, FL, MN, WA, TN, USDA) 5. Community and stakeholder engagement a. Compile database of registered pesticides b. Maximize the exposure of our livestock entomology research and extension information to our stakeholders through electronic and print communication. c. Educate our stakeholders and funding decision-makers. d. Seek funding to support these extension/outreach efforts by developing proposals that will be submitted to various granting agencies including our Regional IPM Centers.
Measurement of Progress and Results
- Develop the first in depth evaluation of the natural product GRAS repellents for use on livestock. We will develop technology to increase the longevity of natural repellents and we will further understand of how flies respond to repellents.
- Develop and evaluate recognition software to estimate fly population densities on livestock
- Add more than a dozen new compounds not currently registered for use on livestock to the current list of potential registrations. Identify new compounds to use in novel application technology such as the VetCap system. Develop a refined novel IGR autodissemination method for use in livestock systems and sugar baits for control of stable flies.
- Develop more efficacious use and methods of evaluation for biological control agents, particularly the entomopathogenic fungi and hymenopteran parasitoids in livestock systems. Provide recommendations for alternative bedding materials to minimize fly development and optimal trapping strategies for target species.
- We will produce research and extension publications describing the mechanism(s) of resistance to insecticides in house flies, stable flies and horn flies. A database of resistance mechanisms by fly species and geographic distribution will be made available to support management efforts directed at house flies and stable flies.
- 2b. We will develop a publicly-accessible database of the house fly and stable fly genomes that can be queried by fly researchers from various scientific disciplines.
- 3a. Identification of attractive and repellent compounds from stable fly larval development substrates and from bacteria associated with flies and their development sites. Attractive lures will be developed and tested based upon fly response to these compounds. A database of bacterial, viral, and fungal taxa associated with blow flies will be developed.
- 3b. We will produce research and extension publications describing infection of house flies by bacteria and other pathogens of concern to humans
- 3c. The geographic distribution and incidence of salivary gland hypertrophy virus infection of house flies in the U.S. will be plotted. We will evaluate, develop and recommend selected essential oils with documented antimicrobial or microstatic activity against mastitis in dairy cattle.
- 4a. Datasets will be produced and made available describing the dispersal distance of stable flies with additional recorded data on environmental factors that affect dispersal.
- 4b. A population growth model for stable fly using time-series datasets will be provided to collaborators for use in further research or extension to growers and other extension clientele.
- 4c. A database of larval development site characteristics will be produced and made available.
- 5a. A searchable, national database of all pesticides registered in the US for use in animal agriculture will be compiled and updated annually. To maximize usefulness for the end-user, pesticide products will be searchable by a number of descriptors, including active ingredient, product name, state registrations, usage area, mode of action, and target pest.
- 5b. A national website with links to all currently available livestock entomology research and extension information from across the US will be made available to our stakeholders.
- 5c. We will hire a temporary "popular science" writer to prepare articles for distribution to our stakeholders. We will prepare displays and written materials and exhibit/distribute these at state, regional and national meetings/conferences to increase stakeholder awareness of our livestock entomology research and extension efforts. We will collaborate on developing regular state, regional and national updates for user groups (Extension agents, producer groups, veterinarians, etc.) through conference calls and/or on-line conferencing utilities. We will conduct pest specific webinars for farmers/stakeholders (conventional and organic), private practice and st
Outcomes or Projected Impacts
- 1a. Adoption of the push pull strategy for the management of pasture flies will result in significant reduction of insecticide use on animals and in the environment. This will reduce the amount of insecticide entering our food chain and greater food safety.
- 1b. Development of efficient pest monitoring tools for livestock will result in increased precision in evaluation of implemented treatment measures.
- 1c. Results of these studies will bring new insecticidal products and repellents to the fore front for use in livestock pest management. Insect growth regulators used in the autodissemination studies will provide a novel treatment for the control of insects using hormone mimics. Results should provide information for use of treated fence for protection of animals from biting and other nuisance flies. It is anticipated that the use of this innovative fencing would help reduce the fly dissemination of pathogens associated with livestock. Publications will include descriptions for use and how to evaluate results. Bait testing results will give producers another method for stable fly control, either alone or in conjunction with attractive traps or targets.
- 1d. Results will represent novel contributions to the knowledge of dairy fly management. In the short term, we expect that dairy farmers will have increased awareness and knowledge of IPM on the dairy. We expect, in the longer term, that there will be increased producer adoption of reduced-risk fly management methods and reduced reliance on pyrethroids and organophosphate insecticides to manage dairy fly pests. A practical method to monitor flies around dairy calves and a broader understanding of the impact flies have on calves. Data from bedding treatment trials will be analyzed and compiled and initially presented as technical reports from which extension and research publications will be developed. This project will provide a thorough comparative evaluation of walk through fly traps. Results will provide producers with data specific to efficacy, bionomics, and economics of using walk through fly traps.
- See attachment for complete Outcomes/Impacts section due to character limit
Milestones(0): attachment for complete Milestones section due to character limit
Projected ParticipationView Appendix E: Participation
Ahmad A, Nagaraja TG, Zurek L. 2007. Transmission of Escherichia coli O157: H7 to cattle by house flies. Prevent. Vet. Med. 80:74-81.
Alam M.J. and L. Zurek 2004. Association of Escherichia coli O157:H7 with house flies (Musca domestica) on a cattle farm. Applied Environ. Microbiol. 70: 7578-7580.
Andress, E. R. and J. B. Campbell. 1994. Inundative releases of pteromalid parasitoids (Hymenoptera: Pteromalidae) for the control of stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae) at confined cattle installations in west central Nebraska. J. Econ. Entomol. 87: 714-722.
Annan, I.B, J.M. Alvarez, H.E. Portillo, R. Edoliya, J. Wiles. 2011. DuPont Cyazypyr" (DPX-HGW86, cyantraniliprole): a novel insecticide for aphid pest management and plant protection. Proceeding of Entomological Society of America.
Axtell, R.C. 1986. Fly management in poultry production cultural biological and chemical. Poultry Science 65: 657-667.
Bach, S.J., T.A. McAllister, D.M. Veira, V.P.J. Gannon, and R.A. Holley. 2002. Transmission and control of Escherichia coli O157:H7- A review. Can J. Anim. Sci. 82: 475-490.
Barraud, N., M.V. Storey, Z.P. Moore, J.S. Webb, S.A. Rice, and S. Kjelleberg. 2009. Nitric oxide-mediated dispersal in single- and multi-species biofilms of clinically and industrially relevant microorganisms. Microbial Biotechnology. 2:370-378.
Barros, ATM, Alison, MW, Jr. and Foil, LD (1999) Evaluation of a yearly insecticidal ear tag rotation for control of pyrethroid-resistant horn flies (Diptera: Muscidae). Vet Parasitol 82: 317-325.
Beerwinkle, K.R., I.L. Berry, and S.E. Kunz. 1978. Prediction models for mortality of immature stable flies caused by cold temperatures. Environ. Entomol. 7:273-277.
Beresford, D. V. and J. F. Sutcliffe. 2010. Assessing pest control using changes in instantaneous rate of population increase: treated targets and stable fly populations case study. J. Dairy Sci. 93: 25172524.
Berkebile, D.R., and G.D. Thomas. 1995. Overwintering and dispersal of the stable fly. Pp. 110-118. In: Thomas, G. D., and S. R. Skoda (Eds.). The Stable Fly: A Pest of Humans and Domestic Animals. Proc. Symposium, 1992 Ann. ESA Meeting. Neb. Agric. Exp. Sta. Misc. Pub. MP 64. 148 pp.
Berkebile, D.R., G.D. Thomas, and J.B. Campbell. 1994. Overwintering of the stable fly (Diptera, Muscidae) in southeastern Nebraska. J. Econ. Entomol. 87:1555-1563.
Berry, I.L., K.W. Foerster, and J.B. Campbell. 1978. Overwintering behavior of stable flies in manure mounds. Environ. Entomol. 7:67-72.
Berry, I.L., K.W. Foerster, E.H. Ilcken. 1976. Prediction model for development time of stable flies. Trans. Am. Soc. Agric. Eng. 19:123-127.
Berry, I. L., S. E. Kunz, and K. W. Foerster. 1977. A dynamic model of the physiological development of immature stable flies. Ann. Entomol. Soc. Am. 70:173-176.
Bloomcamp, CL, Patterson, RS and Koehler, PG (1987) Cyromazine resistance in the house fly (Diptera: Muscidae). J Econ Entomol 80: 352-357.
Broce, A.B., J. Hogsette, and S. Paisley. 2005. Winter feeding sites of hay in round bales as major developmental sites of Stomoxys calcitrans (Diptera: Muscidae) in pastures in Spring and Summer. J. Econ. Entomol. 98: 2307-2312.
Bruce, W.G. 1938. A practical trap for the control of horn flies on cattle. J. Kansas Entomol. Soc. 11: 88-93.
Bruce, W.G. 1940. A cattle trap for the control of horn flies. U.S. Dept. of Agri. E-498.
Bruce, W.G. 1964. The history and biology of the horn fly, Haematobia irritans (L.) with comments on control. N. C. Agric. Exp. Stn. Tech. Bull. 157.
Bull, D.L. and Meola, R.W. 1994. Efficacy and toxicodynamics of pyriproxifen after treatment of insecticide-susceptible and -resistant strains of the house fly (Diptera: Muscidae). J. Econ. Entomol. 87, 1407-1415.
Byford, RL, Craig, ME, Derouen, SM, Kimball, MD, Morrison, DG, Wyatt, WE and Foil, LD (1999) Influence of permethrin, diazinon and ivermectin treatments on insecticide resistance in the horn fly (Diptera: Muscidae). Intern J Parasitol 29: 125-135.
Campbell, J.B., S.R. Skoda, D.R. Berkebile, and G.D. Thomas. 2001a. Research on stable flies and house flies at Nebraska. Res. Bull. #341, Agr. Res. Div., IANR, Univ. of Nebraska-Lincoln. 22 pp.
Campbell, J.B., R.G. White, J.E. Wright, R. Crookshank, and D.C. Clanton. 1977. Effects of stable flies on weight gains and feed efficiency of calves on growing or finishing rations. J. Econ. Entomol. 70:592-594.
Cancado, F.C., Effio, P. C., Terra, W.R. and S.R. Marana. 2008. Cloning, purification and comparative characterization of two digestive lysozymes from Musca domestica larvae. Braz. J. Med. Biol. Res. 41: 969-977.
Castro, E., A. Gil, M. A. Solari, and N. A. Farias. 2005. Validation of a subjective counting method for horn flies (Haematobia irritans irritans) ( Diptera: Muscidae) populations in a cattle herd. Vet. Parasitol. 133:363-367.
Castro, B.G., M.M.S. de Souza, and A.J. Bittencourt. 2007. Aerobic bacterial microbiota in Stomoxys calcitrans: Preliminary study in Brazil. Rev. Bras. Parasitol. Vet. 16: 193-197.
Castro, B.G., M.M.S. de Souza, A.H. Regua-Mangia, and A.J. Bittencourt. 2010. Transbound. Emerg. Dis. 57: 22-24.
Cook, S.M., Z.R. Khan, and J.A. Pickett. 2007. The use of push-pull strategies in integrated pest management. Annu. Rev. Entomol. 52: 375-400.
Crespo, D.C., E.E. Lecuona and J.A. Hogsette. 2002. Strategies for controlling house fly populations resistant to cyromazine. Neotrop. Entomol. 31: 141-147.
Crespo, D.C., R.E. Lecuona and J.A. Hogsette. 1998. Biological control: an important component in integrated management of Musca domestica (Diptera: Muscidae) in caged-layer poultry houses in Buenos Aires, Argentina. Biol. Control 13: 16-24.
Denis, M., J. Refregier-Petton, M. Laisney, G. Ermel and G. Salvat. 2001. Campylobacter contamination in French chicken production from farm to consumers. Use of a PCR assay for detection and identification of Campylobacter jejuni and Campylobacter coli. J. Appl. Microbiol. 91: 255-267.
Devine, G. J., E. Z. Perea, G. F. Killeen, J. D. Stancil, S. J. Clark, and A. C. Morrison. 2009. Using adult mosquitoes to transfer insecticides to Aedes aegypti larval habitats. Proc. Natl. Acad. Sci. USA 106: 11530-11534.
Doucet, D and Retnakaran, A (2012) Insect chitin: metabolism, genomics and pest management. Advances in Insect Physiology 43: 437-511.
Doud, C. and L. Zurek. 2012. Enterococcus faecalis OG1RF:pMV158 survives and proliferates in the house fly digestive tract. J. Med. Entomol. 49: 150-155.
Drummond, R. O., G. Lambert, H. E. Smalley, and S. E. Terrill.1981. Estimated losses of livestock to pests. In: D. Pimentel (Ed.) CRC Handbook of Pest Management in Agriculture. CRC Press, Boca Raton, FL.
Drummond, R. 1988. Economic aspects of ectoparasites of cattle in North America. pp. 9-24. In The economic impact of parasitism in cattle. Proceedings World Vet. Cong. Lawrenceville, NJ.
Dryden, M.W. 1998. Laboratory evaluations of topical flea control products. In: Proc. 1998 British Veterinary Dermatology Study Group. April 1, 1998. Birmingham, United Kingdom. pp. 14-17.
Edwards, J. F., S. E. Wikse, R. W. Field, C. C Hoelscher and D. B. Herd. 2000. Bovine teat atresia associated with horn fly (Haematobia irritans irritans L.) induced dermatitis. Vet. Pathol. 37: 360-364.
Elder, R.O., J.E. Keen, G.R. Siragusa, G.A. Barkocy-Gallagher, M. Koohmaraie, and W.W. Laegreid. 2000. Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. PNAS 97: 2999-3003
Erickson, M.C., M. Islam, C. Sheppard, J. Liao, and M.P. Doyle. 2004. Reduction of Escherichia coli O157:H7 and Salmonella enterica Serovar enteritidis in chicken manure by larvae of the black soldier fly. J. Food Prot. 67:685-690.
Ferreira de Almeida, M.A., and A. Pires do Prado. 1999. Aleachara spp. (Coleoptera: Staphylinidae) and pupal parasitoids (Hymenoptera: Pteromalidae) attacking symbovine fly pupae (Diptera: Muscidae, Sarcophagidae and Otitidae) in Southeastern Brazil. Biol. Control 14: 77-83.
Floate, K, B. Khan, and G. Gibson. 1999. Hymenopterous parasitoids of filth fly (Diptera: Muscidae) pupae in cattle feedlots. Can. Entomol. 131: 347-362.
Foil, L.D. and C.D. Younger. 2006. Development of treated targets for the control of stable flies (Diptera: Muscidae). Vet. Parasitol. 137: 311-315.
Foil, L.D., Guerrero, F., Alison, M.W. and Kimball, M.D. (2005) Association of the kdr and superkdr sodium channel mutations with resistance to pyrethroids in Louisiana populations of the horn fly, Haematobia irritans irritans (L.). Vet Parasitol 129: 149-158.
Foil, L.D., Guerrero, F.D. and Bendele, K.G. (2010) Detection of target site resistance to pyrethroids and organophosphates in the horn fly using multiplex PCR. J Med Ent 47: 855-861.
Fradin, M.S. and J.F. Day. 2002. Comparative efficacy of insect repellents against mosquito bites. New Engl. J. Med. 347: 13-18
Gannon, V. P., S. DSouza, T. Graham, R. K. King. K. Rahn, and S. Read. 1997. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J. Clinic. Microbiol. 35: 656-662.
Gaugler, R., D. Suman and Y. Wang. 2011. An autodissemination station for the transfer of an insect growth regulator to mosquito oviposition sites. Med. Vet. Entomol. doi: 10.1111/j.1365-2915.2011.00970.x
Geden, C.J. 2005. Methods for monitoring outdoor population of house flies, Musca domestica L. (Diptera: Muscidae). J. Vector Ecol. 30: 244-250.
Geden, C.J. 2012. Status of biopesticides for control of house flies. Journal of Biopesticides.5, Issue SUPPL., 1-11
Geden, C.J. and J.A. Hogsette [eds.]. 1994. Research and Extension Needs for Integrated Pest Management of Arthropods of Veterinary Importance. Proceedings of a Workshop in Lincoln, Nebraska (Last Updated October 2001). Accessible at: http://www.ars.usda.gov/Services/docs.htm?docid=10139
Geden, C. J. and J. A. Hogsette. 2006. Suppression of house flies (Diptera: Muscidae) in Florida poultry houses by sustained releases of Muscidifurax raptorellus and Spalangia cameroni (Hymenoptera: Pteromalidae). Environ. Entomol. 35: 75-82.
Geden, C.J., D.A. Rutz, R.W. Miller, and D.C. Steinkraus. 1992a. Suppression of house flies (Diptera: Muscidae) on New York and Maryland dairies using releases of Muscidifurax raptor (Hymenoptera: Pteromalidae) in an integrated management program. Environ. Entomol. 21: 1419-1426.
Geden, C. J., D. C. Steinkraus, R. W. Miller and D. A. Rutz. 1992b. Suppression of house flies on New York and Maryland dairies using Muscidifurax raptor in an integrated management program. Environ. Entomol. 21: 1419-1426.
Gerry, AC and Zhang, D (2009) Behavioral resistance of house flies, Musca domestica (Diptera: Muscidae) to imidacloprid. United States Army Med Depart J: 54-59.
Gerry, A.C., G. E. Higginbotham, L. N. Periera, A. Lam, and C.R. Sheton. 2011. Evaluation of surveillance methods for monitoring house fly abundance and activity on large commercial dairy operations. J. Med. Entomol. 104:1093-1102.
Gilles J., I. Litrico, P. Sourrouille, G. Duvallet. 2004. Microsatellite DNA markers for the stable fly, Stomoxys calcitrans (Diptera : Muscidae). Molecular Ecology Notes 4: 635-637.
Gillespie, B. E., W. E. Owens, S. C. Nickerson and S. P. Oliver. 1999. Deoxyribonucleic acid fingerprinting of Staphylococcus aureus from heifer mammary secretions and from horn flies. J. Dairy Sci 82: 1581-1585.
Glass, H.W., R.R. Gerhardt and W.H. Greene. 1982. Survival of Moraxella bovis in the Alimentary Tract of the Face Fly. J Econ. Entomol. 75: 545-546
Glass, H.W. and R.R. Gerhardt. 1984. Transmission of Moraxella bovis by Regurgitation from the Crop of the Face Fly (Diptera: Muscidae). J. Econ. Entomol. 77: 399-401.
Goulson, D., L.C. Derwtby, M.E. Hanley, D.W. Dunn, and S.R. Abolins. 2005. Predicting calyptrate fly populations form the weather, and probable consequences of climate change. J. Appl. Ecol. 42: 795804.
Graczyk, T.K., et al., The role of non-biting flies in the epidemiology of human infectious diseases. Microbes and Infection, 2001. 3(3): p. 231-235.
Greenberg, B. 1971. Flies and disease, Vol. 1. Princeton University Press, Princeton, New Jersey, 856 pp.
Greenberg, B. 1973. Flies and disease, Vol. II. Princeton Univ. Press, Princeton, New Jersey, 447 pp.
Greenberg, B., J.A. Kowalski, and J.M. Klowden. 1970. Factors affecting the transmission of Salmonella by flies: natural resistance to colonization and bacterial interference. Infect. Immun. 2: 800-809.
Greene, G.L. 1989. Seasonal population trends of adult stable flies. Pp. 1217 in Petersen J.J. and G.L. Greene (eds), Current status of stable fly (Diptera: Muscidae) research. Misc. Publ. Entomol. Soc. Am., No. 74. 53 pp.
Guerra, L., J.G. Stoffolano, Jr., G. Gambellini, V. Laghezza Masci, M.C. Belardinelli and A.M. Fausto. 2012. Ultrastructure of the salivary glands of non-infected and infected glands in Glossina pallidipes by the salivary glands hypertrophy virus. J. Invert. Pathol., in press.
Guo, Yu-Jie, J. A. Hogsette, G. L. Greene, and C. J. Jones. 1997. Survey report on pupal parasites of filth flies in livestock and poultry facilities in China. Chinese J. Biol. Control 13: 106-109. (in Chinese).
Hall, R.D. and K.E. Doisy. 1989. Walk-through trap for control of horn flies (Diptera: Muscidae) on pastured cattle. J. Econ. Entomol. 82: 530-534.
Hall, K.D., R.L. Holloway, C.E. Hoelscher, and J.C. Paschal. 1996. Texas Beef Cattle (Cow / Calf and Stocker) 1994 Pest Control Survey. B-6045. Texas Agricultural Extension Service, Texas A&M University. 14 pp.
Hamm, R.L., T. Shono, and J.G. Scott. 2005. A North-South cline in frequency of autosomal males is not associated with insecticide resistance in housefly, Musca domestica L. J. Econ. Entomol. 98: 171-176.
Hammer. K. A., C. F. Carlson, and T. V. Riley. 1999. Antimicrobial activity of essential oils and other plant extracts. J. Appl. Microbiolo. 86: 985-990.
Hansens, E.J. 1951. The stable fly and its effect on seashore recreational areas in New Jersey. J. Econ. Entomol. 44: 482-487.
Hatakoshi, M., H. Kawada, S. Nishida, H. Kisida, and I. Nakayama. 1987. Laboratory evaluation of 2-[1-methyl-2-(4-phenoxyphenoxy)-ethoxy] pyridine against larvae of mosquitoes and housefly. Jpn. J. Sanit. Zool. 38: 271-274.
Havron, A. and J. Margalit. 1991. Pupal parasitoids (Hymenoptera: Pteromalidae) of muscoid filth flies in Israel. Med. Vet. Entomol 5: 267-275.
Hinkle, N.C. and L.A. Hickle. 1999. California caged layer pest management evaluation. J. Appl. Poult. Res. 8: 327-338.
Hogsette, J., R. Jacobs, and R. Miller. 1993. The Sticky Card: Device for Studying the Distribution of Adult House Fly (Diptera: Muscidae) Populations in Closed Poultry Houses" (1993). J. Econ. Entomol. 86:450-454.
Hogsette, J. A., R. Farkas, and C. Thuroczy. 2001. Hymenopteran pupal parasitoids recovered from house fly and stable fly (Diptera: Muscidae) pupae collected on livestock facilities in Southern and Eastern Hungary. Environ. Entomol. 30: 107-111.
Hogsette, J.A., and J.P. Ruff. 1985. Stable fly (Diptera: Muscidae) migration in northwest Florida. Environ. Entomol. 14: 170-175.
Hu, Y., Q. Zhang, and J.C. Meitzer. 1999. Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by multiplex PCR. J. Appl. Microbiol. 87: 867-876.
Invest, J. F. and J .R. Lucas. 2008. Pyriproxyfen as a mosquito larvicide. In Proceedings of the Sixth International Conference on Urban Pests, pp. 239-245.
Edited by W. H. Robinson & D. Bajomi. Veszprem, Hungary: OOK-Press Kft.
Iseki, A and Georghiou, GP (1986) Toxicity of cyromazine to strains of the housefly (Diptera: Muscidae) variously resistant to insecticides. J. Econ. Entmol. 79: 1192-1195.
Jones, C. J. and R. A. Weinzierl. 1997. Geographical and temporal variation in pteromalid (Hymenoptera: Pteromalidae) parasitism of stable fly and house fly (Diptera: Muscidae) pupae collected from Illinois cattle feedlots. Environ. Entomol. 42: 421-432.
Jonsson, N. N., and D. G. Mayer. 1999. Estimation of the effects of buffalo fly (Haematobia irritans exigua) on the milk production of dairy cattle based on a meta-analysis of literature data. Med. Vet. Entomol. 13: 372376.
Juan, L.W., Zerba, E.N., Mariategui, P., Speicys, C., Tarelli, G., Demyda, S., Masuh, H.M. 2010. New spot-on formulation containing chlorpyrifos for controlling horn flies on cattle: Laboratory model of insecticide release and field trial. Parasitology Research. 107(4), 967-974.
Kaufman, P.E., and D.A Rutz. 2001. Susceptibility of house flies (Diptera: Muscidae) exposed to commercial insecticides on painted and unpainted plywood panels. Pest. Manag. Sci. 58:174-178.
Kaufman, PE, AC Gerry, DA Rutz, and JG Scott. 2007. Monitoring susceptibility of house flies (Musca domestica L.) in the United States to imidacloprid. J. Agric. Urban Entomol. 23: 195-200.
Kaufman, P. E., Long, S. J., Rutz, D. A. and J. K Waldron. 2001. Parasitism rates of Muscidifurax raptorellus and Nasonia vitripennis (Hymenoptera: Pteromalidae) after individual and paired releases in New York poultry facilities. J. Econ. Entomol. 94: 593-598.
Kaufman, PE, SC Nunez, CJ Geden, and ME Scharf. 2010. Selection for resistance to imidacloprid in the house fly (Diptera: Muscidae). J. Econ. Entomol. 103: 1937-1942.
Kawada, H., K. Dohara, and G. Shinjo. 1987. Evaluation of larvicidal potency of insect growth regulator, 2- 1-methyl-2-(4-phenoxyphenoxy)ethoxy pyridine, against the housefly, Musca domestica. Jpn. J. Sanit. Zool. 38: 317-322.
Khater, H.F. , Hanafy, A., Abdel-Mageed, A. D., Ramadan, M. Y., El-Madawy, R. S. 2011. Control of the myiasis-producing fly, Lucilia sericata, with Egyptian essential oils. International Journal of Dermatology. 50(2), Pages 187-194.
Kinzer, H. G., J. M. Reeves, and J. W. Atmar. 1978. Host location by the horn fly: Field evaluation of an artificial device for measuring attraction to various stimuli. Environ. Entomol. 7: 375-378.
Kobayashi, M., Sasaki, T., Saito, N., Tamura, K., Suzuki, K., Watanabe, H., and N. Agui. 1999. Houseflies: not simple mechanical vectors of enterohemorrhagic Escherichia coli O157:H7. Am. J. Trop. Med. Hyg. 61: 625-629.
Kozaki, T., SG Brady, and JG Scott. 2009. Frequencies and evolution of organophosphate insensitive acetylcholinesterase alleles in laboratory and field populations of the house fly, Musca domestica L. Insect. Mol. Biol. 95: 6-11.
Krafsur, E.S. and R.D. Moon. 1997. Bionomics of the face fly, Musca autumnalis. Annu. Rev. Entomol. 42: 503-23.
Kristensen, M. and J.B. Jespersen. 2003. Larvicide resistance in Musca domestica (Diptera: Muscidae) populations in Denmark and establishment of resistant laboratory strains. J. Econ. Entomol. 96: 1300-1306.
Kunz, S.E., I.L. Berry, and K.W. Foerster. 1977. The development of the immature forms of Stomoxys calcitrans. Ann. Entomol. Soc. Am. 70:169-172.
Kunz, S.E., K.D. Murrel, G. Lambert, L.F. James, and C.E. Terrill. 1991.
Estimated losses of livestock to pests, In D. Pimentel [ed.], CRC Handbook of Pest Management in Agriculture, vol. I. CRC Press, Boca Raton, FL. pp. 69-98.
Legner, E. F. and G. S. Olton. 1971. Distribution and relative abundance of dipterous pupae and their parasitoids in accumulations of domestic animal manure in the southwestern United States. Hilgardia 40:505-535.
Lemaitre, B., and J.A. Hoffmann. 2007. The host defense of Drosophila melanogaster. Annu. Rev. Immunol. 25:697-743.
Levot, G. and N. Sales. 2004. Insect growth regulator cross-resistance studies in eld- and laboratory-selected strains of the Australian sheep blowy, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae). Austral. J. Entomol. 43: 374377.
Li, AY, KH Lohmeyer, and JA Miller. 2009. Dynamics and mechanisms of permethrin resistance in a field population of the horn fly, Haematobia irritans irritans. Insect Sci. 16: 175-184.
Li, A.Y., D. Ross, and A. A. Perez De Leon. 2011. Evaluation of the VetCap® treatment method for horn fly control in cattle. Int. J. of Appl. Res. Vet. Med. 9(2):198-203.
Li X, Degain BA, Harpold VS, Marçon PG, Nichols RL, Fournier AJ, Naranjo SE, Palumbo JC, Ellsworth PC.2012. Baseline susceptibilities of B- and Q-biotype Bemisia tabaci to anthranilic diamides in Arizona. Pest Manag. Sci. 68(1):83-91.
Likirdopulos, C.A., O.D. Simmons, III, D.W. Watson, and M.D. Sobsey. 2005. Collection methods for evaluating microbial indicator concentrations of house flies (Musca domestica) on swine farms in eastern North Carolina. Proceedings on the Development of Alternative Technologies for the Processing and Use of Animal Waste. 2005 Animal Waste Symposium, Oct. 5-7 Research Triangle Park, NC http://www.cals.ncsu.edu/waste_mgt/05wastesymposium/proceedings.htm
Liscia, A., P. Solari, S.T. Gibbons, A. Gelperin and J.G. Stoffolano, Jr. 2012. Effect of serotonin and calcium on the supercontractile muscles of the adult blowfly crop. Journal of Insect Physiology 58: 356-366.
Liu, Q., J.K. Tomberlin, J.A. Brady, M.R. Sanford, and Z. Yu. 2008. Black Soldier Fly (Diptera: Stratiomyidae) larvae reduce Escherichia coli in dairy manure. Environ. Entomol. 37:1525-1530.
Lohmeyer, KH and Pound, JM (2012) Laboratory evaluation of novaluron as a development site treatment for controlling larval horn flies, house flies, and stable flies (Diptera: Muscidae). J Med Entomol 49: 647-51.
Lole, M.J. 2005. Nuisance flies and landfill activities: an investigation at a West Midlands landfill site. Waste Management Res. 23: 420-428.
Lysyk, T.J. 1993. Adult resting and larval developmental sites of stable flies and house flies (Diptera: Muscidae) on dairies in Alberta. J. Econ. Entomol. 86:1746-1753.
Lysyk T.J. 1998. Relationships between temperature and life-history parameters of Stomoxys calcitrans (Diptera: Muscidae). J. Med. Entomol. 35: 107119.
Lysyk, T. J. 1999. Effect of temperature on time to eclosion and spring emergence of diapausing horn flies, Haematobia irritans (L.) (Diptera: Muscidae). Environ. Entomol. 28: 387-397.
Lysyk, T. J., and R. C. Axtell. 1986. Field evaluation of three methods for monitoring populations of house flies (Musca domestica) (Diptera: Muscidae) and other filth flies in three types of poultry housing systems. J. Econ. Entomol. 79: 144-151.
Lysyk, T.J., and R.C. Axtell. 1996. Movement and distribution of house flies (Diptera: Muscidae) between two habitats in two livestock farms. J. Econ. Entomol. 79: 993-998.
Lysyk, T.J., L. Kalischuk-Tymensen, L.B. Selinger, R.C. Lancaster, L.Wever, and K.-J. Cheng. 1999. Rearing stable fly larvae (Diptera: Muscidae) on an egg yolk medium. J. Med. Entomol. 36: 382-388.
McKay, T. and T.D. Galloway. 1999. Survey and release of parasitoids (Hymenoptera) attacking house and stable flies (Diptera: Muscidae) in dairy operations. Can. Entomol. 131. no. 6, pp. 743-756.
Mead, P.S., L. Slutsker, V. Dietz, L. McCaig, J. Bresee, C. Shaprio, P. Griffin, and R. Tauxe. 1999. Food-related illness and death in the United States. Emerg. Infect. Dis. 5: 607-625.
Meyer, H. J., D. R. Carey, D. D. Kopp, and V. L. Anderson. 1989. A Walk-through fly trap to reduce horn flies on pastured cattle. North Dakota State University Extension, August 1989.
Meyer, J.A., G.P. Georghiou, and M.K. Hawley. 1987. Housefly (Diptera, Muscidae) resistance to permethrin on southern California dairies. J. Econ. Entomol. 80: 636-640.
Meyer, J.A., B.A. Mullens, T.L. Cyr, and C. Stokes. 1990. Commercial and naturally occurring fly parasitoids (Hymenoptera: Pteromalidae) as biological control agents of stable flies and house flies (Diptera: Muscidae) on California dairies. J. Econ. Entomol. 83: 799-806.
Meyer, J.A., T.A. Shultz, C. Collar, and B.A. Mullens. 1991. Relative abundance of stable fly and house fly (Diptera: Muscidae) pupal parasites (Hymenoptera: Pteromalidae; Coleoptera: Staphylinidae) on confinement dairies in California. Environ. Entomol. 20: 915-921.
Mihok, S., E. K. Kangethe, G. K. Kamau. 1995. Trial of traps and attractants for Stomoxys spp. (Diptera: Muscidae). J. Med. Entomol. 32: 283-289.
Miller, R.W. and D.A. Rutz. 1990. Survey of house fly pupal parasitoids on dairy farms in Maryland & New York, pp 59-66. In D.A. Rutz & R.S. Patterson (eds.), Biocontrol of Arthropods Affecting Livestock & Poultry. Westview Press, Boulder, CO. 316 pp.
Monteiro, M.R. and A. Pires do Prado. 2000. Trichopria sp. (Hymenoptera: Diapriidae) attacking pupae of Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae) in a poultry facility. An. Soc. Entomol. Bras. 1: 159-167.
Moon, R.D. 2002. Muscid Flies (Muscidae), pp. 279-303. In G. R. Mullen and L. A. Durden [eds.], Medical and Veterinary Entomology. Academic Press, San Diego.
Moon, R.D., D.M. Noetzel, and L.J. Johnston. 1993. Intake and efficacy of methoprene and stirofos mineral blocks for control of horn flies (Diptera: Muscidae) on pastured beef cattle. J. Econ. Entomol. 86: 1738-1745.
Moore, S.J., A. Lenglet and N. Hill. 2007. Plant-based insect repellents. In Insect Repellents: Principles, methods and uses. Eds. Mustapha Debboun, Stephen Frances and Daniel Strickman. CRC Press. pp. 275-304.
Moore, S.J., and M. Debboun. 2007. History of insect repellents. In Insect Repellents: Principles, methods and uses. Eds. Mustapha Debboun, Stephen Frances and Daniel Strickman. CRC Press. pp. 3-30.
Moreland, T.W., L.G. Pickens, and R.W. Miller. 1995. Livestock walkthrough fly trap. Patent No. 5:419076. Assignee. The United States of America (Washington, DC) and the University of Maryland. (College Park, MD). U.S. Patent and Trademark Office, Washington, DC.
Morgan, P.B. and R.S. Patterson. 1990. Efficiency of target formulations of pesticides plus augmentative releases of Spalangia endius Walker (Hymenoptera: Pteromalidae) to suppress populations of Musca domestica L. (Diptera: Muscidae) at poultry ranches in the southeastern United States, pp. 69-78 In: D. A. Rutz & R. S. Patterson (eds). Biocontrol of arthropods affecting livestock and poultry, Westview, Boulder.
Mullens, B.A., and N.G. Peterson. 2005. Relationship between rainfall and stable fly (Diptera: Muscidae) abundance on California dairies. J. Med. Entomol. 42: 705708.
Olafson, PU, JB Pitzer, and PE Kaufman. 2011. Identification of a mutation associated with permethrin resistance in the para-type of sodium channel of the stable fly (Diptera: Muscidae). J. Econ. Entomol. 104: 250-257.
Oliver, S.P., B.E. Gillespie, S.J. Headrick, M.J. Lewis and H.H. Dowlen. 2005. Prevalence, risk factors and strategies for controlling mastitis in heifers during the periparturient period. Intern J. Appl. Res. Vet Med. 3: 150-162.
Owens, C.B., and A.L. Szalanski. 2005. Filter paper for preservation, storage and distribution of insect and pathogen DNA samples. J. Med. Entomol. 42: 709-711.
Ozoe Y, Asahi M, Ozoe F, Nakahira K, Mita T. 2010. The antiparasitic isoxazoline A1443 is a potent blocker of insect ligand-gated chloride channels. Biochem. Biophys. Res. Commun. 391:744-749.
Petera, C. 2010. Biofilms are a key challenge in treating chronic wounds. Microbe. 5:414-416.
Petersen, J. J. and D. W. Watson. 1992. Comparison of sentinel and naturally occurring fly pupae to measure field parasitism by pteromalid parasitoids (Hymenoptera). Biol. Control 2:224-248.
Petersen, J. J. and J. A. Meyer. 1983. Host preference and seasonal distribution of pteromalid parasites (Hymenoptera: Pteromalidae) of stable flies and house flies (Diptera: Muscidae) associated with confined livestock in eastern Nebraska. Environ. Entomol. 12: 567-571.
Petersen, J. J. and J. K. Cawthra. 1995. Release of a gregarious Muscidifurax species (Hymenoptera: Pteromalidae) for the control of filth flies associated with confined beef cattle. Biol. Control 5: 279-284.
Pickett, J. A. et al. 1997. Developing sustainable pest control from chemical ecology. Ag. Eco. & Environ. 64: 149-156.
Pitzer, JB, PE Kaufman, and SH TenBroeck. 2010. Assessing permethrin resistance in the stable fly (Diptera: Muscidae) in Florida using laboratory selections and field evaluations. J. Econ. Entomol. 103: 2258-2263.
Regnault-Roger, C., C. Vincent, and J. T. Arnason. 2012. Essential oils in insect control: Low-risk products in a high-stakes world. Ann. Rev. Entomol. 57, 405-424.
Rinkevich, FD, RL Hamm, CJ Geden, and JG Scott. 2007. Dynamics of insecticide resistance alleles in two different climates over an entire field season. Insect Biochem. Mol. Biol. 37: 550-558.
Rinkevich, FD, Hedtke, SM, Leichter, CA, Harris, SA, Su, C, G., BS, Taskin, V, Qiu, X and Scott, JG (2012) Multiple origins of kdr-type resistance in the house fly, Musca domestica. PLoS One 7: e52761.
Rinkevich, FD, L Zhang, RL Hamm, SG Brady, BP Lazzaro, and JG Scott. 2006. Frequencies of the pyrethroid resistance alleles of Vssc1 and CYP6D1 in house flies from the eastern United States. Insect. Mol. Biol. 15: 157-167.
Roberts, T. 2007. WTP estimates of the societal costs of U.S. food-borne illness. Amer. J. Agr. Econ. 89:1183-1188.
Romero , A., A. Broce, and L. Zurek. 2006. Role of bacteria in the oviposition behavior and larval development of stable flies. Med. Vet. Entomol. 20: 115-121.
Romero, A., J. A. Hogsette, and A. Coronado. 2010. Distribution and abundance of natural parasitoid (Hymenoptera: Pteromalidae) populations of house flies and stable flies (Diptera: Muscidae) at the University of Florida Dairy Research Unit. Neotrop. Entomol. 39: 424-429.
Romeo, Y. and B. Lemaitre. Drosophila immunity. Methods Mol. Biol. 415: 379-394.
Rueda, L. M., P. U. Roh and J. L. 1997. Pupal parasitoids (Hymenoptera: Pteromalidae) of filth flies (Diptera: Muscidae, Calliphoridae) breeding in refuse and poultry and livestock manure in South Korea. J. Med. Entomol. 34: 82-85.
Rutz, D. A and R. C. Axtell. 1981. House fly (Musca domestica L.) control in broiler-breeder poultry houses by pupal parasites (Hymenoptera: Pteromalidae): indigenous parasite species and releases of Muscidifurax raptor. Environ. Entomol. 10: 343-345.
Rutz, D. A. and R. C. Axtell. 1980. House fly parasites (Hymenoptera: Pteromalidae) associated with poultry manure in North Carolina. Environ. Entomol. 9:175-180.
Sabatini, GA, PEM Ribolla, ATM Barros, FD Guerrero, and TTS Schumaker. 2009. Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil. Rev. Bras. Parasitol. Vet. 18: 8-14.
Sanderson MW, JM Sargeant, X Shi, TG Nagaraja, L Zurek, and MJ Alam. 2006. Longitudinal emergence and distribution of Escherichia coli O157 genetic strains in a beef feedlot. Appl. Environ. Microbiol. 72: 7614-7619.
Sasaki, T., M. Kobayashi, and N. Agui. 2000. Epidemiological potential of excretion and regurgitation by Musca domestica (Diptera: Muscidae) in the dissemination of Escherichia coli O157:H7 to food. J. Med. Entomol. 37: 945-949.
Scharff, R.L. 2010. Health-related costs from foodborne illness in the United States, in Produce Safety Project. Washington, D.C.
Schmidtmann, E.T. 1988. Exploitation of bedding in dairy outdoor calf hutches by immature house and stable flies (Diptera: Muscidae). J. Med. Entomol. 25:484-488.
Schmidtmann, E.T., R.W. Miller and R. Muller. 1989. Effect of experimental bedding treatments on the density of Iimmature Musca domestica and Stomoxys calcitrans (Diptera: Muscidae) in outdoor calf hutches. J. Econ. Entomol. 82: 1134-1139.
Schmidtmann, E.T. 1991. Suppressing immature house and stable flies in outdoor calf hutches with sand, gravel, and sawdust bedding. J. Dairy Sci. 74: 3956-3960.
Scholl, P.J., J.J. Petersen, D.A. Stage, and J.A. Meyer. 1981. Open silage as an overwintering site for immature stable flies in eastern Nebraska. Southwest. Entomol. 6: 253-258.
Schurrer, J.A., S.A. Dee, R.D. Moon, K.D. Rossow, C. Mahlum, E. Mondaca, S. Otake, E. Fano, J. Collins, and C. Pijoan. Spatial dispersal of porcine reproductive and respiratory syndrome virus-contaminated flies after contact with experimentally infected pigs. Amer. J. Vet. Res. 65: 1284-1292.
Scott, J.G., R.T. Roush, and D.A. Rutz. 1989. Insecticide resistance of house flies from New York dairies (Diptera: Muscidae). J. Agric. Entomol. 6: 53-64.
Scott J.G., T.G. Alefantis, P.E. Kaufman, and D.A. Rutz. 2000. Insecticide resistance in house flies from caged-layer poultry facilities. Pest. Manag. Sci. 56:147-153.
Seng, C., T. Setha, J. Nealon, D. Socheat, and M. B. Nathan. 2008. Six months of Aedes aegypti control with a novel controlled-release formulation of pyriproxyfen in 32 domestic water storage containers in Cambodia. Southeast Asian J. Trop. Med. Publ. Health 39: 822-826.
Shen, J and FW Plapp Jr. 1990. Cyromazine resistance in the house fly (Diptera: Muscidae): genetics and cross-resistance to diflubenzuron. J. Econ. Entomol. 83: 1689-1697.
Skovgård, H and J. B. Jespersen. 2000. Seasonal and spatial activity of hymenopterous pupal parasitoids (Pteromalidae and Ichneumonidae) of the house fly (Diptera: Muscidae) on Danish pig and cattle farms. Environ. Entomol. 29: 630-637.
Skovgård, H. and G. Nachman. 2004. Biological control of house flies Musca domestica and stable flies Stomoxys calcitrans (Diptera: Muscidae) by means of inundative releases of Spalangia cameroni (Hymenoptera: Pteromalidae). Bull. Entomol. Res. 94: 555567.
Skovgård, H. and J. B. Jespersen. 1999. Activity and relative abundance of hymenopterous parasitoids that attack puparia of Musca domestica and Stomoxys calcitrans (Diptera: Muscidae) on confined pig and cattle farms in Denmark. Bull. Entomol. Res. 89: 263-269.
Skovgård, H. and T. Steenberg. 2002. Activity of pupal parasitoids of the stable fly Stomoxys calcitrans and prevalence of entomopathogenic fungi in the stable fly and the house fly Musca domestica in Denmark. BioControl 47: 45-60.
Somme, L. 1961. On the overwintering of house flies (Musca domestica (L.)) and stable flies (Stomoxys calcitrans (L.)) in Norway. Norsk. Entomol. Tidsskr. 11:191-223.
Srinivasan, R. and N. Balakrishnan. 1989. Preliminary note on the parasitoids of Musca domestica (Diptera: Muscidae) in Pondicherry. Entomon 14: 349-352.
Stoffolano, J.G., Jr., A.M. Fausto, M. Carcupino, G. Gambellini and L. Guerra. 2010. The diverticulated crop of adult Phormia regina. Arthropod Structure & Development. 39: 251-260.
Sulaiman, S., B. Omar, S, Omar, J. Jeffery, I. Ghauth, and V. Busparani. 1990. Survey of microhymenoptera (Hymenoptera: Chalcidoidea) parasitizing filth flies (Diptera: Muscidae, Calliphoridae) breeding in refuse and poultry farms in Peninsular Malaysia. J. Med. Entomol. 27: 851-855.
Surgeoner, G. A., S. M. Butler, L. R. Lindsay And J. D. Heal. 1998. Efficacy and feasibility of a walk through fly trap for control of nuisance flies on dairy cattle in Ontario. Dept. of Environ. Biolo., University of Guelph. Ontario, Canada. http://22.214.171.124/beefupdate/articles96/aefficacy_and_feasibility_of_a_wa.htm
Sutherst, R.W. and R.S. Tozer. 1995. Control of buffalo fly (Haematobia irritans exigua de Meijere) on dairy and beef cattle using traps. Aust. J. Agric. Res. 46: 269-284.
Szalanski, A.L., C.B. Owens, T. McKay and C.D. Steelman. 2004. Detection of Campylobacter and Escherichia coli O157:H7 from filth flies by polymerase chain reaction. Med. Vet. Entomol. 18:241-246.
Talley, J., A. Broce, and L. Zurek. 2009. Characterization of stable fly (Diptera: Muscidae) larval developmental habitat at round hay bale feeding sites. J. Med. Entomol. 46: 1310-1319.
Taylor, D. B., R.D. Moon, J. B. Campbell, D. R. Berkebile, P. J. Scholl, A. B. Broce, and J. A. Hogsette. 2010. Dispersal of stable flies (Diptera: Muscidae) from larval development sites in a Nebraska landscape. Environ. Entomol. 39: 1101-1110.
Taylor, D.B., K. Hale, J.J. Zhu, and K. Sievert. 2012. Efficacy of cyromazine to control immature stable flies (Diptera: Muscidae) developing in winter hay feeding sites. J. Econ. Entomol. 105: 726-35.
Taylor, D.B., R.D. Moon, and D.R. Mark. 2012. Economic impact of stable flies (Diptera: Muscidae) on dairy and beef cattle production. J. Med. Entomol. 49:198-209.
Temeyer, KB, Li, AY, Lohmeyer, KH, Chen, AC, Olafson, PU, Sanson, DW and Foil, LD (2008) Acetylcholinesterase mutation in diazinon-resistant Haematobia irritans (L.) (Diptera: Muscidae). Vet. Parasitol. 154: 300-310.
Thomas, G.D., and S.R. Skoda. [eds.]. 1993. Rural flies in the urban environment? Proceedings of a symposium presented at the Annual Meeting of the Entomological Society of America, December, 1989 San Antonio, Texas. Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, Neb.
Tozer, R.S. and R.W. Sutherst. 1996. Control of horn fly (Diptera: Muscidae) in Florida with an Australian trap. J. Econ. Entomol. 89: 415-420.
Turchin, P., and W. T. Thoeny. 1993. Quantifying dispersal of southern pine beetles and mark-recapture experiments and a diffusion model. Ecol. Appl. 3: 187-198.
US Patent 5205063. 1993. Inventors: R.W. Sutherst and R.S. Tozer.
Voegtline, A.C., G.W. Ozburn, and G.D. Gill. 1965. The relation of weather to biting activity of Stomoxys calcitrans (L.) along Lake Superior. Papers Mich. Acad. Sci. Arts Letters. 50: 107-114.
Watson, D. W., S. M. Stringham, S. S. Denning, S. P. Washburn, M. H. Poore, and A. Meier. 2002. Managing the horn fly, Haematobia irritans (L.), Diptera, Muscidae, using an electric walk-through fly trap. J. Econ. Entomol. 95: 1113-1118.
Watson, D.W., P.A.W. Martin, and E.T. Schmidtmann. 1993. Egg yolk and bacteria growth medium for Musca domestica (Diptera: Muscidae). J. Med. Entomol. 30: 820-823.
Weinzierl, R. A. and C. J. Jones. 1998. Releases of Spalangia nigroaenea and Muscidifurax zaraptor (Hymenoptera: Pteromalidae) increase rates of parasitism and total mortality of stable fly and house fly (Diptera: Muscidae) pupae in Illinois cattle feedlots. J. Econ. Entomol. 91: 1114-1121
Wellington, W.G. 1945. Conditions governing the distribution of insects in the free atmosphere. I-IV. Canad. Entomol. 77: 7-15, 21-28, 44-49, 69-74.
Winpisinger, K.A., A.K. Ferketich, R.L. Berry, and M.L. Moeschberger. 2005. Spread of Musca domestica (Diptera: Muscidae), from two caged layer facilities to neighboring residences in rural Ohio. J. Med. Entomol. 42: 732-73.
WHO. 2009. Food safety and foodborne illness. http://www.who.int/mediacentre/factsheets/fs237/en/.
Zhu, J.J., A.Y. Li, S. Pritchard, K. Tangtrakulwanich, F.P. Baxendale, and G. Brewer. 2011. Contact and fumigant toxicity of a botanical-based feeding deterrent of the stable fly, Stomoxys calcitrans (Diptera: Muscidae). J. Agric. Food Chem. 59:10394-10400.