W3171: Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock

(Multistate Research Project)

Status: Inactive/Terminating

SAES-422 Reports



Rorie, R.W., A. J. Davis, T. D. Lester, and J. G. Powell. 2014. Comparison of two estrous synchronization protocols for use with X sorted semen in lactating beef cows. Prof. Anim. Sci. 30:620-624.

Rorie, R.W., P.A. Delgado and T.D. Lester. 2014. Variation among beef bulls in the ratio of X- to Y-chromosome bearing spermatozoa. Adv. Reprod. Sci. 2:69-75.

Rowe, M.P., J.G. Powell, E.B. Kegley, T.D. Lester and R.W. Rorie. 2014. Effect of supplemental trace mineral source on bull semen quality. Prof. Anim. Sci. 30:68-73.

Bakhtari A, Ross PJ. DPPA3 prevents cytosine hydroxymethylation of the maternal pronucleus and is required for normal development in bovine embryos. Epigenetics. 2014 Sep;9(9):1271-9. doi: 10.4161/epi.32087. Epub 2014 Aug 1.

Berger, T., and A. Conley. 2014. Reduced endogenous estrogen and hemicastration interact synergistically to increase porcine sertoli cell proliferation. Biology of reproduction 90: 114.

Reyes JM, Chitwood JL, Ross PJ. RNA-Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation. Mol Reprod Dev. 2015 Jan 5. doi: 10.1002/mrd.22445.

Campos-Chillon F, Farmerie TA, Bouma GJ, Clay CM, Carnevale EM. The effect of aging on gene expression and mitochondrial DNA in the equine oocyte and follicle. Reprod Fertil & Develop, 2015 Mar 19. doi: 10.1071/RD14472.

da Silveira JC, Winger QA, Bouma GJ, Carnevale EM. Age effects on follicular fluid exosomal miRNAs and TGF? signaling during follicle development in the mare. Reprod Fertil & Develop, Accepted.

Cleys ER, Halleran JL, Enriquez VA, da Silveira JC, West RC, Winger QA, Anthony RV, Bruemmer JE, Clay CM, Bouma GJ. Androgen Receptor and Histone Lysine Demethylases in Ovine Placenta. PLoS ONE 2015 Feb 12;10(2):e0117472..

Hatzel JN, Bouma GJ, Cleys ER, Bemis LT, Ehrhart EJ, McCue PM. Identification of heat shock protein 10 within the equine embryo, endometrium, and maternal peripheral blood mononuclear cells. Theriogenology 2015 Mar 15;83(5):832-9..

Nemere I, Garbi N, Winger Q. The 1,25D3 -MARRS Receptor/PDIA3/ERp57 and Lifespan. J Cell Biochem. 2014 Oct 6. doi: 10.1002/jcb.24986. [Epub ahead of print]

Cleys ER, Halleran JL, McWhorter E, Hergenreder J, Enriquez VA, da Silveira JC, Bruemmer JE, Winger QA, Bouma GJ. Identification of microRNAs in exosomes isolated from serum and umbilical cord blood, as well as placentomes of gestational day 90 pregnant sheep. Mol Reprod Dev. 2014 Nov;81(11):983-93.

da Silveira JC, Carnevale EM, Winger QA, Bouma GJ. Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare. Reprod Biol Endocrinol. 2014 May 26;12:44.

Szerlong H, Herman JA, Krause CM, Deluca JG, Skoultchi A, Winger QA, Prenni JE, Hansen JC. Proteomic characterization of the nucleolar linker histone H1 interaction network. Journal of Molecular Biology, J Mol Biol. 2015 Jan 10. pii: S0022-2836(15)00006-6.

Jeckel KM, Bouma GJ, Hess AM, Petrilli EB, Frye MA. Dietary fatty acids alter left ventricular myocardial gene expression in Wistar rats. Nutr Res. 2014 Aug;34(8):694-706.

Le CH, Mulligan CM, Routh MA, Bouma GJ, Frye MA, Jeckel KM, Sparagna GC, Lynch JM, Moore RL, McCune SA, Bristow M, Zarini S, Murphy RC, Chicco AJ. Delta-6-desaturase links polyunsaturated fatty acid metabolism with phospholipid remodeling and disease progression in heart failure. Circ Heart Fail. 2014 Jan;7(1):172-83.

Lin, CJ; Amano, T; Tang Y; TianXC. 2014. Improved derivation efficiency and pluripotency of stem cells from the refractory inbred C57BL/6 mouse strain by small molecules. PLoSONE 9(9): e106916 published online Sept 11, 2014.

Jiang Z, Sun J, Dong H, Luo O, Zheng X, Obergfell C, Tang Y, Bi J, O’Neill R, Ruan Y, Chen J, Tian XC. (2014) Complete Transcriptional Profiles of Bovine In Vivo Pre-implantation Development. BMC Genomics 15:756; published online Sept 4, 2014.

Tang Y, Jiang Z, Luo Y, Zhao X, Wang L, Tian XC. (2014). Differential Effects of Akt Isoforms on Somatic Cell Reprogramming J Cell Sci 127:3998-4008.

Zopf D.A., Flanagan, C.L., Wheeler, M.B., Hollister, S.J., Green, G.E. 2013. Treatment of severe porcine tracheomalacia with a 3-dimensionally printed, bioresorbable, external airway splint. JAMA Otolaryngol Head Neck Surg. 2014 Jan;140(1):66-71.

Yuan, Y., Paczkowski, M., Wheeler, M.B., Krisher, R.L. 2014. Use of a novel polydimethylsiloxane well insert to successfully mature, culture and identify single porcine oocytes and embryos. Reproduction, Fertility and Development 26:375-384.

Monzani, P.S., Guemra, S., Adona, P.A., Ohashi, O.M., Meirelles, F.V., Wheeler, M.B. 2014. MAC-T cells as a tool to evaluate lentiviral vector construction targeting recombinant protein expression in milk. Animal Biotechnology, 26:2, 136-142.

Zopf D.A., Mitsak, A.G., Flanagan, C.L., Wheeler, M.B., Green, G.E., Hollister, S.J. 2014. Computer-Aided Designed, 3-Dimensionally Printed Porous Tissue Bioscaffolds For Craniofacial Soft Tissue Reconstruction. J Otolaryngol Head Neck Surg. Online: DOI: 10.1177/0194599814552065

Bondioli, K.R. Cryopreservation of Bovine Embryos. In: Hopper, R. (ed) Bovine Reproduction. John Wiley and Sons, 2015 pp. 718-722.

Chen, H., Fu, J., Chen, H., Hu, Y., Soroka, D. N., Prigge, J. R., Schmidt, E. E., Yan, F., Major, M. B., Chen, X. and Sang, S. (2014) Ginger compound [6]-shogaol and its cysteine-conjugated metabolite (M2) activate Nrf2 in colon epithelial cells in vitro and in vivo. Chemical research in toxicology. 27, 1575-1585.

Sonsteng, K. M., Prigge, J. R., Talago, E. A., June, R. K. and Schmidt, E. E. (2014) Hydrodynamic delivery of Cre protein to lineage-mark or time-stamp mouse hepatocytes in situ. PLoS ONE. 9, e91219

Gorrini, C., Gang, B. P., Bassi, C., Wakeham, A., Baniasadi, S. P., Hao, Z., Li, W. Y., Cescon, D. W., Li, Y. T., Molyneux, S., Penrod, N., Lupien, M., Schmidt, E. E., Stambolic, V., Gauthier, M. L. and Mak, T. W. (2014) Estrogen controls the survival of BRCA1-deficient cells via a PI3K-NRF2-regulated pathway. Proc Natl Acad Sci USA. 111, 4472-4477

Saunders G, Stevens JR, and Isom SC. A shortcut for multiple testing on the directed acyclic graph of Gene Ontology. BMC Bioinformatics 2014, 15:349 DOI: 10.1186/s12859-014-0349-3

Li Q, Suasnavas E, Xiao L, Heywood S, Qi X, Zhou A, Isom SC. Label-free and non-invasive monitoring of porcine trophoblast derived cells: differentiation in serum and serum-free media. J Biophotonics. 2014 Sep 22;9999(9999). doi:10.1002/jbio.201400062.

Suasnavas E, Heywood S, Ward A, Cox L, O’Grady M, Zhao Y, and Isom SC. Characterization of trophoblast-derived stem-like cells from peri-implantation porcine embryos. Animal Reproduction Science. In Press.

Ni W., Qiao J., Hu S., Zhao X., Regouski M., Yang M., Polejaeva I.A.*, Chen C.* Efficient gene knockout in goats using CRISPR/Cas9 system. PLoS One 2014, 9 (9), e106718.

Gong J., Chen G., Wang Z., Polejaeva I., Silisga R., Chen C-T., Kao C-M., and Chen L. Activating the expression of human K-rasg12d stimulates oncogenic transformation in transgenic goat fetal fibroblast cells. PLoS ONE. 2014, 9(3): e90059.

Zhou W.*, Gosch G., Guerra T., Broek D., Wu G., Walker S., Polejaeva I*. Amino acid profiles in first trimester amniotic fluids of healthy cloned pregnancies are similar to those of IVF pregnancies, but not nonviable cloned pregnancies. Theriogenology. 2014, 81 (2), 225-229


W-3171 Publications


Chen H, Fu J, Chen H, Hu Y, Soroka DN, Prigge JR, Schmidt EE, Yan F, Major MB, Chen X, Sang S. 2014.  Ginger compound [6]-shogaol and its cysteine-conjugated metabolite (M2) activate Nrf2 in colon epithelial cells in vitro and in vivo. Chem Res Toxicol. 2014 Sep 15;27(9):1575-85.

Godke, RA, M Sansinena, and CR Youngs. 2014.  Assisted reproductive technologies and embryo culture methods for farm animals. In (CA Pinkert, Ed.) Transgenic Animal Technology: A Laboratory Handbook, 3rd Ed., Elsevier, London, pp. 581-638.  DOI: http://dx.doi.org/10.1016/B978-0-12-410490-7.00022-0

Vivanco, W, D Ponce, M Miguel, C Youngs, E Huamán, S. León, and M Asparrin.  2014.  Repeatability of the ovarian response and embryo production in superovulated Huacaya alpacas. Spermova 4(1):77-79.

Youngs CR. 2014. Embryo development and survival in ruminant livestock species. In (G. Gutierrez and M Rothschild, Eds.). Proceedings of the International Seminar on Advances in Animal Production in Latin America, November 5-7, 2014, Lima Peru, pp. 40-51.


Abedal-Majed, M.A., M.P. Magamage, R.J. Vinton, R.A. Cushmann, A.K. McNeel, H. Freetly, J.R. Wood, and A.S. Cupp. 2015. Vascular Endothelial Growth Factor A 165 (VEGFA165), angiogenic isoform, promotes while VEGFA165b antagonizes VEGFA165 stimulated follicular progression in bovine ovarian cortical pieces cultured from pre-pubertal heifers. Society for the Study of Reproduction 47th Annual Meeting. San Juan, Puerto Rico. p. 246.

Bionaz, M., Monaco, E.  and Wheeler, M.B. 2015. Transcription Adaptation during In Vitro Adipogenesis and Osteogenesis of Porcine Mesenchymal Stem Cells: Dynamics of Pathways, Biological Processes, Up-Stream Regulators, and Gene Networks. PLoS One. 2015 Sep 23;10(9):e0137644. doi: 10.1371/journal.pone.0137644. eCollection 2015.

Bondioli, K.R.  Cryopreservation of Bovine Embryos. 2015.  In: Hopper, R. (ed) Bovine Reproduction. John Wiley and Sons, pp. 718-722.

Campos-Chillon F, Farmerie TA, Bouma GJ, Clay CM, Carnevale EM. 2015. The effect of aging on gene expression and mitochondrial DNA in the equine oocyte and follicle. Reprod Fertil & Develop, Mar 19. doi: 10.1071/RD14472. [Epub ahead of print].

Canovas S and Ross PJ. 2015.  Mechanisms of epigenetic remodeling during preimplantation development. Reproduction, Fertility and Development. 28(2): 25-40.

Cebula M, Schmidt EE, Arnér ES.  2015.  TrxR1 as a potent regulator of the Nrf2-Keap1 response system.. Antioxid Redox Signal. 23(10):823-53.

Cederberg, R.A., J.E. Smith, E.A. McDonald, C. Lee, A.R. Perkins, and B.R. White. 2015. Activity of the porcine gonadotropin-releasing hormone receptor gene promoter is partially conferred by a distal gonadotrope specific element (GSE) within an upstream enhancing region, two proximal GSEs and a retinoid X receptor binding site. Reprod. Biol. Endocrinol. 13:45.

Center, K., D. Dixon, and R. Rorie.  2015.  Anti-mullerian hormone and follicle counts as predictors of superovulatory response and embryo production in beef cattle. Arkansas Agri. Exp. Sta. Res. Series 628:31-33.

Cleys ER, Halleran JL, Enriquez VA, da Silveira JC, West RC, Winger QA, Anthony RV, Bruemmer JE, Clay CM, Bouma GJ. 2015. Androgen Receptor and Histone Lysine Demethylases in Ovine Placenta. PLoS ONE 10(2):e0117472. [PMID: 25675430]

Cox L, Hintze KJ, and ISOM SC.  June 2015.  Impact of a Total Western Diet on oocyte quality in swine.  48th Annual Meeting of the Society for the Study of Reproduction.  San Juan, PR. 

Cox L, ISOM SC, and Hintze KJ.  April 2015.  Formulation of the Total Western Diet (TWD) for Pigs and Phenotypic Differences After a 12-week Feeding Trial.  29th Annual Meeting of the Federation of American Societies for Experimental Biology.  Boston, MA. 

Cushman, R.A., R.G. Tait Jr., A.K. McNeel, E.D. Forbes, O.L. Amundson, C.A. Lents, A.K. Lindholm-Perry, G.A. Perry, J.R. Wood, A.S. Cupp, T.P. Smith, H.C. Freetly, and G.L. Bennett. 2015. A polymorphism in myostatin influences puberty but not fertility in beef heifers, whereas µ-calpain affects first calf birth weight. J. Anim. Sci. 93:117-26.

da Silveira JC, Winger QA, Bouma GJ, Carnevale EM. 2015.  Age effects on follicular fluid exosomal miRNAs and TGFβ signaling during follicle development in the mare. Reprod Fertil & Develop, May 7. doi: 10.1071/RD14452. [Epub ahead of print].

Davis, A.J., T.D. Lester, E.A. Backes and R.W. Rorie.  2015.  Sequential use of Estrotect estrous detection patches as a reproductive management tool.  Prof. Anim. Sci. 31:50-56. 

Davis, A.J., T.D. Lester, E.A. Backes and R.W. Rorie.  2015.  Serial use of Estrotect estrous detection patches as a reproductive management tool.  J. Anim. Sci., So. Sec. (abstr 38, pg 16)

Desaulniers, A.T., R.A. Cederberg, G.A. Mills, C.A. Lents and B.R. White. 2015. A putative role for GnRH-II and its receptor in spermatogenic function of boars. Society for the Study of Reproduction 47th Annual Meeting. San Juan, Puerto Rico. p. 210.

Desaulniers, A.T., R.A. Cederberg, G.A. Mills, J.J. Ford, C.A. Lents, and B.R. White. 2015. LH-independent testosterone secretion is mediated by the interaction between GNRH2 and its receptor within porcine testes. Biol. Reprod. 93:45.

Diaz, FA, EJ Gutierrez, BA Foster, PT Hardin and KR Bondioli.  2015.  Effect of the Repeated Use of Open System Vitrification Devices on MII Stage and Cleavage Rates of Bovine Cumulus-oocyte Complexes.  Reproduction, Fertility and Development 28:145.

Dodson, M.V.,  R.E. Allen, M. Du, W.G. Bergen, S.G. Velleman, S.P. Poulos, M. Fernyhough-Culver, M.B. Wheeler, S.K. Duckett, M.R.I. Young, B.H. Voy, Z. Jiang and G.J. Hausman, 2015. Invited Review: Evolution of meat animal growth research during the past 30 years: Adipose and muscle stem cells. J. Anim. Sci. 2015.93:457–481.

Douthit, C.J., R.A. Cederberg, A.T. Desaulniers, J.M. Kouba, T.L. Douthit, D.M. Grieger and B.R. White. 2015. Identification of GnRH isoforms and their cognate receptors in the equine testis. Equine Science Society Annual Meeting. St. Pete Beach, Florida. p. 138.

Ebramzadeh, E., Sangiorgio, S.N., Wheeler, M.B.,  Green, G.E. 2015. Design Control for Clinical Translation of 3D Printed Modular Scaffolds. Annals of Biomedical Engineering, Vol. 43, No. 3,774–786.

Enriquez VA, Cleys ER, da Silveira JC, Spillman MA, Winger QA, Bouma GJ. 2015. High LIN28A Expressing Ovarian Cancer Cells Secrete Exosomes That Induce Invasion and Migration in HEK293 Cells. BioMed Res Int 2015, Article ID 701390.

Eriksson S, Prigge JR, Talago EA, Arnér ES, Schmidt EE. 2015.  Dietary methionine can sustain cytosolic redox homeostasis in the mouse liver.  Nat Commun. 6:6479.

Eriksson, S, and Schmidt, E.E.  2015.  “Thioredoxin reductase: A coordinator of metabolic activities”. In “Selenium, its molecular biology and role in human health”. Edited by Brigelieus-Flohe, R. Within series entitled: “Oxidative stress in health and disease”. CRC Press.

Ferré LB, Bogliotti Y, Chitwood JL, Rodríguez CF ,Ortega HH, Kjelland ME and Ross PJ.  2015. Comparison of different fertilization media for an in vitro maturation/fertilization/culture system using flowcytometrically sorted X-chromosome-bearing sperm for bovine embryo production. Reproduction, Fertility and Development. May 13 Epub ahead of print.

Ferreira, C.R., Jarmusch, A.K., Pirro, V., Alfaro, C.M., González-Serrano, A.F., Niemann, H., Wheeler, M.B., Rabel, R.A.C., Hallett, J.E., Kaufmann, A. and Cooks, R.G.  2015.  Ambient Ionization Mass Spectrometry for Lipid Profiling and Structural Analysis of Oocytes, Preimplantation Embryos and Stem Cells. Reproduction, Fertility and Development, 27:621–637.

Foster, BA, FA Diaz, PT Hardin, EJ Gutierrez and KR Bondioli.  2015.  Characterization of Bovine Oocyte Cytoplasmic Maturation with Common IVM Protocols.  Reproduction, Fertility and Development  28:232

Hatzel JN, Bouma GJ, Cleys ER, Bemis LT, Ehrhart EJ, McCue PM. 2015. Identification of heat shock protein 10 within the equine embryo, endometrium, and maternal peripheral blood mononuclear cells. Theriogenology 83(5):832-839. [PMID: 25542459]

Herzog, K.K., Milner, D.J., Johnson, S.J., Wheeler, M.B.  2015. Comparison of Chondrogenic Potential of Porcine Adipose-Derived Stem Cells, Chondrocytes, Periosteal Cells, and Fibroblasts in a Pellet Culture System. Reproduction, Fertility and Development 27(1) 253-253.

Hollister, S.J., Flanagan, C.L., Zopf, D.A., Morrison, R.J., Nasser, H.,  Patel, J.J., Hughes JR, Berger T.  2015.  Testicular fluid regulates apical blebbing in the porcine epididymis. American Society of Andrology.

Hughes JR, Berger T. 2015. Development of apical blebbing in the boar epididymis. PLOS One 2015.

Jahnke MM, JK West, and CR Youngs.  2015.  Evaluation of in vivo derived bovine embryos. In (RM Hopper, Ed) Bovine Reproduction, Wiley & Sons, Hoboken NJ, pp 733-748.

Jiang Z, Harrington P, Zhang M, Marjani SL, Park J, Kuo L, Pribenszky C, Tian XC. 2015. Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts (accepted by Scientific Reports, Dec 2015).

Jiang Z, Tang Y, Zhao X, Zhang M, Donovan DM, Tian XC. 2015.  Knockdown of Brm and Baf170, Components of Chromatin Remodeling Complex, Facilitates Reprogramming of Somatic Cells. Stem Cells and Development 24:2328-36.

Katleba K, Legacki EL, Conley AJ, Berger T.  2015.  Steroid Regulation of Early Postnatal Development in the Corpus Epididymidis of Pigs. Journal of Endocrinology 225(3): 125-134.

Klohonatz KM, Hess AM, Hansen TR, Squires EL, Bouma GJ, Bruemmer JE. 2015. Equine endometrial gene expression changes during and after maternal recognition of pregnancy. J Anim Sci 93(7):3364-3376.

Lambe-Steinmiller, J.C.  2015.  The Use of Assisted Reproductive Technologies to Improve Pregnancy Rates in White-tailed Deer.  Louisiana State University. PhD dissertation

Legacki E, Conley AJ, Nitta-Oda BJ, Berger T.  Porcine Sertoli Cell Proliferation after Androgen Receptor Inactivation. Biology of Reproduction. 2015; 92(4): 93.

Li Q, Suasnavas E, Heywood S, Xiao L, Zhou A, Isom SC.  2015.  Biochemical, biophysical and genetic changes of porcine trophoblast-derived stem-like cells during differentiation as evaluated using Raman microspectroscopy, Atomic force  microscopy and quantitative polymerase chain reaction. Genesis. 2015 Oct 28. doi: 10.1002/dvg.22907. [Epub ahead of print] PubMed PMID: 26509257

Li Q, Suasnavas E, Williams S, Li R, ISOM SC, and Zhou A.  2015  Atomic force microscopy (AFM), Raman Microspectroscopy (RM) and gene chip monitoring of porcine trophoblast derived cell differentiation.  SPIE BiOS Conference on Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII.  San Francisco, CA

Meyer, L., T. Devine, M. Looper, D. Philipp, D. Hubbell, III, R. Rorie, and C. Rosenkrans, Jr.  2015.  Associations between heifer endocrine profiles and reproductive efficiency.  Arkansas Agri. Exp. Sta. Res. Series 628:38-39.

Nemere I, Garbi N, Winger Q. 2015. The 1,25D3 -MARRS receptor/PDIA3/ERp57 and lifespan. J Cell Biochem. 116(3):380-5. doi: 10.1002/jcb.24986. PMID: 25283641

Non-Invasive Quantification of Cartilage Using a Novel In Vivo Bioluminescent Reporter Mouse. Mailhiot SE, Zignego DL, Prigge JR, Wardwell ER, Schmidt EE, June RK. 2015.  PLoS One. 2015 Jul 7;10(7):e0130564.

Park JP, Lai L, Samuel MS, Wax D, Prather RS, Tian XC. 2015. Disruption of mitochondria-to-nucleus interaction in deceased cloned piglets. PLoS ONE 2015

Prigge JR, Hoyt TR, Dobrinen E, Capecchi MR, Schmidt EE, Meissner N. 2015. Type I IFNs Act upon Hematopoietic Progenitors To Protect and Maintain Hematopoiesis during Pneumocystis Lung Infection in Mice.  J. Immunol. 195(11):5347-57.

Romereim, S., A.F. Summers, B.E. Pohlmeier, R.M. McFee, R. Spuri-Gomes, S.G. Kurz, A.K. McNeel, R.A. Cushmann, J.S. Davis, J.R. Wood, and A.S. Cupp. 2015. Granulosa cell cycle regulation and steroidogenesis in a high androstenedione follicular microenvironment. Society for the Study of Reproduction 47th Annual Meeting. San Juan, Puerto Rico. p. 245.

Rubessa, M., Ambrosi, A., Polkoff, K.M., Stewart, J.W., Herzog, K.K., Denmark, S.E. Wheeler, M.B. 2015. Metabolic Profiling of Male and Female Bovine Embryos Using Nuclear Magnetic Resonance (NMR) Imaging. Reproduction, Fertility and Development 27(1) 158–159.


Rubessa, M., Herzog, K.K., Ambrosi, A., Stewart, J.W., Polkoff, K.M., Denmark, S.E. Wheeler, M.B. 2015. Non-Invasive Analysis of Embryo Metabolites Using Nuclear Magnetic Resonance. Reproduction, Fertility and Development  27(1) 157–157.

Rubessa, M., Nasser, L.F., Wheeler, M.B. 2015. Practical Applications of In Vitro Embryo Production in Latin America. Proceedings 11th Simposium Internacional de Reproduction Animal, Cordoba, AR.

Rutigliano HM, Wilhelm A, Hall J, Shi B, Meng Q, Stott R, Bunch TD, White KL, Davies CJ, Polejaeva IA. Cytokine gene expression at the maternal-fetal interface after somatic cell nuclear transfer pregnancies in small ruminants. Reprod Fertil Dev. 2015 Oct 14. doi: 10.1071/RD15103. [Epub ahead of print] PubMed PMID: 26463834.

Ryu J and Lee K.  2015. Complete disruption of RAG2 gene during porcine embryogenesis utilizing CRISPR/Cas9 system. Biol Reprod. (Suppl. 1): 377.

Sanz-Fernandez MV, Johnson JS, Abuajamieh M, Stoakes SK, Seibert JT, Cox L, Kahl S, Elsasser TH, Ross JW, ISOM SC, Rhoads RP, Baumgard LH.  2015.  Effects of heat stress on carbohydrate and lipid metabolism in growing pigs.  Physiol Rep. 3(2) . pii: e12315. doi: 10.14814/phy2.12315. Print 2015 Feb 1.  PMID: 25716927

Sargent, K.A., R. Spuri-Gomes, J.R. Essink, S.G. Kurz, W.E. Pohlmeier, N. Ferrara, G.J. Bouma, D.J. McLean, and A.S. Cupp.  2015. Sertoli cell-specific elimination of Vegfa impairs spermatogonial stem cell (SSC) maintenance and reduces fertility in male mice. Society for the Study of Reproduction 47th Annual Meeting. San Juan, Puerto Rico. p. 266-267.

Sargent, K.M., N. Lu, D.T. Clopton, W.E. Pohlmeier, V.M. Brauer, N. Ferrara, D.W. Silversides, and A.S. Cupp. 2015. Loss of vascular endothelial growth factor A (VEGFA) isoforms in granulosa cells using pDmrt-1-Cre or Amhr2-Cre reduces fertility by arresting follicular development and by reducing litter size in female mice. PLoS One. 10:e0116332.

Sargent, K.M., R.M. McFee, R. Spuri Gomes, and A.S. Cupp. 2015. Vascular endothelial growth factor A: just one of multiple mechanisms for sex-specific vascular development within the testis? J. Endocrinol. 227:R31-50.

Schmidt EE.  2105.  Interplay between cytosolic disulfide reductase systems and the Nrf2/Keap1 pathway. Biochem Soc Trans. 43(4):632-8.

Spuri-Gomes, R., S. Tenley, M.A. Abedal-Majed, S.G. Kurz, J.R. Wood, J. Bergman, and A.S. Cupp. 2015. Cows with intrafollicular androgen excess have lower sex hormone binding globulin and appear to be chronic or sporadic anovulatory. Society for the Study of Reproduction 47th Annual Meeting. San Juan, Puerto Rico. p. 250.

Suasnavas E, Heywood S, Ward A, Cox L, O’Grady M, Zhao Y, and ISOM SC.  Isolation and characterization of trophoblast-derived stem-like cells from peri-implantation porcine embryos. Anim Reprod Sci. 2015 Mar; 154:128-41. doi: 10.1016/j.anireprosci.2015.01.012. Epub 2015 Jan 31.  PMID: 25660622. 

Szerlong HJ, Herman JA, Krause CM, DeLuca JG, Skoultchi A, Winger QA, Prenni JE, Hansen JC. 2015. Proteomic characterization of the nucleolar linker histone H1 interaction network. J Mol Biol. 427(11):2056-71. doi:10.1016/j.jmb.2015.01.001. Epub 2015 Jan 10. PMID: 25584861

Talbot, H.A., P. Zhang, X. Hou, E. Keiser, F. Yu, A.S. Cupp, and J.S. Davis. 2015. Identification of IL-17 as a potential mediator of luteolysis: Inhibition of progesterone secretion. Society for the Study of Reproduction 47th Annual Meeting. San Juan, Puerto Rico. p. 243.

Tanphaichitr N,  Kongmanas K,Kruevaisayawan H, Saewu A, Sugeng C, Fernandes J, Souda P, Angel JB, Faull KF, Aitken RJ, Whitelegge J, Hardy D, Berger T, Baker M. 2015. Remodeling of the plasma membrane in preparation for sperm-egg recognition: roles of acrosomal proteins. Asian Journal of Andrology 17(4): 1-9.

Thorson, J.F., A.T. Desaulniers, C. Lee, B.R. White, J.J. Ford, C.A. Lents. 2015. The role of RFamide-related peptide 3 (RFRP3) in regulation of the neuroendocrine reproductive and growth axes of the boar. Anim. Reprod. Sci. 159:60-65.

Tian XC. 2015. Establishing the standards of gene expression during bovine pre-implantation development. (presented in the 12th Annual meeting of the Asian Reproductive Biotechnology Society; November 26-29, 2015, Hanoi, Vietnam). P.8

Vivanco-Mackie HW, MDP Salazar, M Miguel, C Youngs, and M Asparrin.  2015.  Embryo survival to calving according to type of embryo and embryo transfer method in alpacas. Reprod Fertil Dev 27(1):173 (abstr).

Wheeler, M.B., Kim, D., Beebe, D. US Patent "Microfluidic Systems and Methods", patent # 9,157,550, issued October 13, 2015

White, B.R. 2015.  Can a “reproductive hormone” predict appetite in first parity, lactating sows? Nebraska Pork Talk, Nebraska Pork Producers Association. 47(3):6-7.

Xie, F., C.L. Anderson, K.R. Timme, S.C. Fernando, and J.R. Wood. 2015. Increased firmicutes in the cecum of obese female mice is correlated with increased Pou5f1 and Dppa3 mRNAs in growing oocytes. Society for the Study of Reproduction 47th Annual Meeting. San Juan, Puerto Rico. p. 248.

Youngs CR and KL Klemesrud. 2015. Update on bovine pregnancy testing technologies [Actualización sobre tecnologías de pruebas de gestación bovina]. Proceedings of the 38th Annual Scientific Meeting of the Peruvian Association of Animal Production, October 7-9, Ayacucho, Peru, pp. 23-28.

Youngs CR, HW Vivanco-Mackie, DP Salazar, M Miguel, M Asparrin. 2015. Recent research on the cryopreservation of preimplantation embryos from South American camelids [Investigaciones recientes sobre la criopreservación de embriones de preimplantación de camélidos sudamericanos]. Proceedings of the 38th Annual Scientific Meeting of the Peruvian Association of Animal Production, October 7-9, Ayacucho, Peru, pp. 18-22.

Youngs CR. 2015. Importance of animal production in global food security [Importancia de la producción animal en la seguridad alementaria mundial]. Proceedings of the 38th Annual Scientific Meeting of the Peruvian Association of Animal Production, October 7-9, Ayacucho, Peru, pp. 14-17.


Acharya, A., J.M. Burke, K. Coffey, and R.W. Rorie.  2016.  Effect of semen extender and storage temperature on ram sperm motility over time.  J. Anim. Sci., So. Sec. (accepted).

Center, K., D. Dixon, and R. Rorie.  2016.  Anti-mullerian hormone as a predictive endocrine marker for superovulatory response and embryo production in beef cattle.  Reprod. Fertil. Dev. (in press)

Cox L, Vanderwall DK, Parkinson KC, Sweat L, and ISOM SC.  Expression profiles of select genes in cumulus-oocyte complexes from young and aged mares.  Reprod Fertil Dev. IN PRESS.  2015 May 15.  doi:  10.1071/RD14446.  PMID:  25976356

Duan JE, Jue NK, Jiang Z, O’Neill R, Wolf E, Blomberg LA, Dong H, Zheng X, Chen J, and Tian XC. 2015. (to be presented in the 42nd Annual Meeting of the International Embryo Transfer Society, Louiville, Kentucky, USA Jan 22-26, 2016).

Jiang Z, Dong H, Zheng X, Donovan DM, Chen J, Tian XC. 2015. mRNA Levels of Imprinted Genes in Bovine In Vivo Oocytes, Embryos and Cross Species Comparisons in Humans, Mice and Pigs (accepted by Scientific Reports, Nov 30, 2015)

Jiang Z, Harrington P, Zhang M, Marjani SL, Park J, Kuo L, Pribenszky C, Tian XC. 2015. Effects of high hydrostatic pressure on expression profiles of in vitro produced, vitrified bovine blastocysts. (to be presented in the 42nd Annual Meeting of the International Embryo Transfer Society, Louiville, Kentucky, USA Jan 22-26, 2016).

Johnson, S., Milner, D., Lopez-Lake, H., Wheeler, M.B.  2016. Chondrogenic pellet cultures for cartilage tissue engineering grow by deposition of matrix and not by cellular proliferation. Reproduction, Fertility and Development, 28, 254-254.

Long JA, HD Blackburn, A Martin, FG Silversides, R Taylor, Jr, and CR Youngs.  2016. The need for agricultural innovation to sustainably feed the world by 2050: Protecting food animal gene pools for future generations. CAST Publication (under review).

Lotti, S.N., Tasan, I., Zhao, H., Wheeler, M.B. 2016.  MAC-T cells as a tool to examine genome editing using clustered regularly interspaced short palindromic repeats (CRISPR). Reproduction, Fertility and Development, 28, 251-251.

Polkoff, K.M., Rubessa, M., Winters, R., Lopez, N.A., Wheeler, M.B. 2016. Comparison of two embedding systems for follicular culture. Reproduction, Fertility and Development, 28, 192-192.

Rodriguez, S.J.R. Ramirez, Y.E., Gomes Jr., E., Nasser, L.F., Pontes, J.H.F., Rubessa, M., Wheeler, M.B. 2016. Comparison of in vitro embryo production in Panama using Holstein oocytes with Bos indicus sexed semen from donors in different locations: field data. Reproduction, Fertility and Development, 28,183-183.

Rorie, R.W., C.L. Williams, and T.D. Lester.  2016.  Association of osteopontin gene promoter single nucleotide polymorphisms with bull semen quality.  Adv. Reprod. Sci. (accepted).

Rubessa, M., Ambrosi, A., Polkoff, K.M., Denmark, S.E., Wheeler, M.B. 2016. Embryo sugar consumption during in vitro development. Reproduction, Fertility and Development, 28, 175-175.

Rubessa, M., Rocha, R., Lima, L., Winters, R., Figueiredo, J.R., Wheeler, M.B. 2016. Comparison of NCSU-23 and alpha-minimal essential media in the development of isolated porcine preantral follicles in vitro. 2016. Reproduction, Fertility and Development, 28,198-198.

Sommer MM and CR Youngs. 2016. Impact of embryo transfer technology on the production of artificial insemination sires for the US dairy cattle industry. Animal Industry Report, Iowa State University (submitted).

Stewart, J., Rubessa, M., Polkoff, K.M., Lotti, S.N., Wheeler, M.B. 2016. Risk of transmission of bovine leukosis virus (BLV) using seropositive bulls for in vitro fertilization embryo production. Reproduction, Fertility and Development, 28, 255-255.

Tavares, K.C.S., Lazzarotto, C.R., Neto, S.G., Martins, L.T., Aguiar, L.H., Calderon, C.E.M., Teixeira, L.P.R., Lopes, F.E.M., Wheeler, M.B., Long, C.R., Whitelaw, B., Bertolini, M., Bertolini, L.R. 2016. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 associated with transient depletion of non-homologous end-joining pathway increased gene-targeting efficiency in goat fibroblasts. Reproduction, Fertility and Development, 28, 252-252.



Baker, C.M., L.N. Goetzmann, J.D. Cantlon, K.M. Jeckel, Q.A. Winger, and R.V. Anthony RV. 2016. Development of ovine chorionic somatomammotropin hormone-deficient pregnancies. Am. J. Physiol. Regul. Integr. Comp. Physiol. 310:R837-R846.


Bogliotti, Y.S., M. Vilarino, and P.J. Ross. 2016. Laser-assisted cytoplasmic microinjection in livestock zygotes. J. Vis. Exp. 116.


Brauer, V.M., J.R. Wiarda-Bell, A.T. Desaulniers, R.A. Cederberg, and B.R. White. 2016.

Functional activity of the Gnrhr2 gene promoter in testis-derived cells is partially conferred

by nuclear factor-kkB, specificity protein 1 and 3 (SP1/3) and overlapping early growth

response 1/SP1/3 binding sites. Gene 587:137-146.


Chio, I.I., S.M. Jafarnejad, M. Ponz-Sarvise, Y. Park, K. Rivera, W. Palm, J. Wilson, V. Sangar,

  1. Hao, D. Öhlund, K. Wright, D. Filippini, E.J. Lee, B. Da Silva, C. Schoepfer, J.E. Wilkinson,

J.M. Buscaglia, G.M. DeNicola, H. Tiriac, M. Hammell, H.C. Crawford, E.E. Schmidt, C.B. Thompson, D.J. Pappin, N. Sonenberg, and D.A. Tuveson. 2016. NRF2 promotes tumor maintenance by modulating mRNA translation in pancreatic cancer. Cell 166:963-976.


Davis, K.A., K.M. Klohonatz, G.J. Bouma, and J.E. Bruemmer. 2016. Androgen receptor in the term equine placenta. J. Eq. Vet. Sci. 54:8-11.


Diaz, F., K. Bondioli, D. Pacamonti, and G.T. Gentry. 2016 .Cryopreservation of Day 8 equine

embryos after blastocyst micromanipulation and vitrification Theriogenology 85:894-903.


Dóka, É., I. Pader, A. Bíró, K. Johansson, Q. Cheng, K. Ballagó, J.R. Prigge, D. Pastor-Flores, T.P. Dick, E.E. Schmidt, E.S. Arnér, and P. Nagy. 2016. A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems. Science Advances. 2:e1500968.


Gladyshev, V.N., E.S. Arnér, M.J. Berry, R. Brigelius-Flohé, E.A. Bruford, R.F. Burk, B.A. Carlson, S. Castellano, L. Chavatte, M. Conrad, P.R. Copeland, A.M. Diamond, D.M. Driscoll, A. Ferreiro, L. Flohé, F.R. Green, R. Guigó, D.E. Handy, D.L. Hatfield, J. Hesketh, P.R. Hoffmann, A. Holmgren, R.J. Hondal, M.T. Howard, K. Huang, H.Y. Kim, I.Y. Kim, J. Köhrle, A. Krol, G.V. Kryukov, B.J. Lee, B.C. Lee, X.G. Lei, Q. Liu, A. Lescure, A.V. Lobanov, J. Loscalzo, M. Maiorino, M. Mariotti, P.K. Sandeep, M.P. Rayman, S. Rozovsky, G. Salinas, E.E. Schmidt, L. Schomburg, U. Schweizer, M. Simonović, R.A. Sunde, P.A. Tsuji, S. Tweedie, F. Ursini, P.D. Whanger, and Y. Zhang. 2016. Selenoprotein gene nomenclature. J. Biol. Chem. 291:24036-24040.


Hill, R., A . Canal, K. Bondioli, R. Morell, and M.D. Garcia. 2016. Molecular markers

located on the DGAT1, CAST, and LEPR genes and their associations with milk production

and fertility traits in Holstein cattle. Genet. Mol. Res. 15:gmr.15017794.


Hollister, S. J., C.L. Flanagan, D.A. Zopf, R.J. Morrison, H. Nasser, M.B. Wheeler, and G.E.

Green. 2016. Design and quality control for translating 3D-printed scaffolds. In:  Essentials of 3D Biofabrication and Translation. Elsevier, Inc. pp. 43-59.


Hollister, S.J., C.L. Flanagan, R.J. Morrison, J.J. Patel, M.B. Wheeler, S.P. Edwards, and G.E. Green. 2016. Integrating image-based design and 3D biomaterial printing to create patient

specific devices within a design control framework for clinical translation. ACS Biomater.

Sci. Eng. 2:1827−1836.


Klohonatz, K.M., A.D. Cameron, J.R. Hergenreder, J.C. da Silveira, A.D. Belk, D.N.R. Veeramachaneni, G.J. Bouma, and J.E. Bruemmer. 2016. Circulating miRNAs as potential alternative cell signaling associated with maternal recognition of pregnancy in the mare. Biol. Reprod. 95:124.


Lan, A., W. Li, Y. Liu, Z. Xiong, X. Zhang, S. Zhou, O. Palko, H. Chen, M. Kapita, J.R. Prigge, E.E. Schmidt, X. Chen, Z. Sun, and X.L. Chen. 2016. Chemoprevention of oxidative stress associated oral carcinogenesis by sulforaphane depends on NRF2 and the isothiocyanate moiety.

Oncotarget. 7:53502-53514.


Legacki, E.L., E.L. Scholtz, B.A. Ball, S.D. Stanley, T. Berger, and A.J. Conley. 2016. The dynamic steroid landscape of equine pregnancy mapped by mass spectrometry. Reproduction 151:421-430.


Lyman, J.T., D.K. Oh, G. Torres, R. Magin, M.B. Wheeler. 2016. The use of nuclear magnetic

resonance spectroscopy for viability predictions on mouse preimplantation embryos. Int. J. New Tech. Res. 2:14-20.


Martins, L.T., S. Gaudêncio Neto, K.C.S. Tavares, C.E.M. Calderón, L.H. Aguiar, C. Lazzarotto, F.L. Ongaratto, V.H.V. Rodrigues, F.E. Lopes, L.P.R. Teixeira, I.S. Carneiro, R. Rossetto, A.P. Almeida, S.S.L. Fernandes, D. Rondina, I.A. Polejaeva, J.L. Rodrigues, F. Forell, L.R. Bertolini, and M. Bertolini. 2016. Developmental outcome and related abnormalities in goats: comparison between somatic cell nuclear transfer- and in vivo-derived concepti during pregnancy through term. Cell. Reprogram. 18:264-279.


McDonald, E.A., J.E. Smith, R. A. Cederberg, and B.R. White. 2016. Divergent activity of

the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is

partially conferred by nuclear factor (NF)-kkB, specificity protein (SP)1-like and GATA-4

binding sites. Reprod. Biol. Endocrinol. 14:36.


Mizell, S., S. Miller, K. Bondioli, and M.D. Garcia. 2016. A comparison of F1 steers sired by

one of three paternal breed types for growth and carcass traits. EC Veterinary Science 2.4:177-181.


Monzani, P.S., P.R. Adona, F.V. Meirelles, O.M. Ohashi, and M.B. Wheeler. 2016. Transgenic

bovine as bioreactor: challenges and perspectives. Bioengineered 7:123-131.


Newberry, H.R., T.D. Lester, B. Kegley, C. Rosenkrans, and R.W. Rorie. 2016. Use of anti-mullerian hormone to select for fertility in beef heifers. Discovery 17:79-84.


Polejaeva, I.A., H.M. Rutigliano, and K. Wells. 2016. Livestock in biomedical research: history,

current status and future prospective. Reprod. Fertil. Dev. 28:112-124.


Polejaeva, I.A., R. Ranjan, C.J. Davies, M. Regouski, J. Hall, A.L. Olsen, Q. Meng, H.M. Rutigliano, D.J. Dosdall, N.A. Angel, F.B. Sachse, T. Seidel, A.J. Thomas, R. Stott, K.E. Panter, P.M. Lee, A.J. Van Wettere, J.R. Stevens, Z. Wang, R.S. MacLeod, N.F. Marrouche, and K.L. White. 2016. Increased susceptibility to atrial fibrillation secondary to myocardial fibrosis in transgenic goats with cardiac specific overexpression of transforming growth factor- B1. J. Cardiovasc. Electrophysiol. 27:1220-1229.


Rorie, R.W., C.L. Williams, and T.D. Lester. 2016. Association of osteopontin gene promoter single nucleotide polymorphisms with bull semen quality. Adv. Reprod. Sci. 4:1-7.


Rubessa, M., A. Ambrosi, D. Gonzalez-Pena, K.M. Polkoff, S.E. Denmark, and M.B. Wheeler.

  1. Non-invasive analysis of bovine embryo metabolites during in vitro embryo culture

using nuclear magnetic resonance. AIMS Bioengineering 3:538-551.


Rubessa, M., A. Ambrosi, S.E. Denmark, and M.B. Wheeler. 2016. Non-invasive analysis of

gamete metabolites during in vitro embryo production using nuclear magnetic resonance.

Int. J. New Tech. Res. 2:54-58.


Rubessa, M., A. Gaja, and M.B. Wheeler. 2016. Separation of motile bovine spermatozoa for in vitro fertilization by electrical charge. Andrology (Los Angel) 5:153.


Sun, J., Z. Jiang, X.C. Tian, and J. Bi. 2016. A cross-species bi-clustering approach to identifying conserved co-regulated genes. Bioinformatics 32:i137-i146.


Shepardson, K.M., K. Larson, R.V. Morton, J.R. Prigge, E.E. Schmidt, V.C. Huber, and A. Rynda-Apple. 2016. Differential type I interferon signaling is a master regulator of susceptibility to postinfluenza bacterial superinfection. MBio 7(3).


Twenter, H.M., A.D. Belk, K.M. Klohonatz, L.D. Bass, G.J. Bouma, and J.E. Bruemmer. 2016. An investigation into miRNAs in the equine epididymis as potential regulators of spermatozoal

maturation. J. Eq. Vet. Sci. 48:61-68.


Vansandt, L.M., J.L. Livesay, J.L., M.J. Dickson, L. Li, B.S. Pukazhenthi, and C.L. Keefer.

  1. Conservation of spermatogonial stem cell marker expression in undifferentiated

felid spermatogonia. Theriogenology 86:1022-1035.


Yang, M., J. Hall, Z. Fan, M. Regouski, Q. Meng, H.M. Rutigliano, R. Stott, K.A. Rood,

K.E. Panter, and I.A. Polejaeva. 2016. Oocytes from small and large follicles exhibit similar

development competence following goat cloning despite their differences in meiotic and

cytoplasmic maturation. Theriogenology 86:2302-2311.


Youngs, C.R. 2016. Modern reproductive technologies to improve cattle production. Ceiba



Berger, T., and B. J. Nitta-Oda. 2018. Increased testicular estradiol during the neonatal interval reduces Sertoli cell numbers.  Anim. Reprod. Sci. 189:146-151.

Bishop, M.O., J.R. Stevens, and S.C. Isom. 2017. Statistical methods for assessing individual oocyte viability through gene expression profiles. Proceedings of the Kansas State University Conference on Applied Statistics in Agriculture 2017.

Bogliotti, Y.S., J. Wu, M. Vilarino, D.A. Soto, J.C. Izpisua Belmonte, and P.J. Ross. 2018. Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts. Proc. Natl. Acad. Sci. 115:2090-2095.

Bondioli, K.R., Cloning of livestock by somatic cell nuclear transfer. 2018. In: Neiman, H. and Wrenzycki, C. (eds). Animal Biotechnology 2: Emerging Breeding Technologies. Springer International Publishing AG. (In Press).

Cadenas, J., C. Maside, A.C.A Ferreira, L.A. Vieira, J. Leiva-Revilla, V.M. Paes, B.G. Alves, F.Z. Brandão, A.P.R. Rodrigues, M.B. Wheeler, and J.R. Figueiredo. 2018. Relationship between follicular dynamics and oocyte maturation during in vitro culture as a non-invasive sign of caprine oocyte meiotic competence. Theriogenology 107:95-103.

Center, K., D. Dixon, C. Looney, and R. Rorie.  2018.  Anti-mullerian hormone and follicle counts as predictors of superovulatory response and embryo production in beef cattle.  Adv. Reprod. Sci. 6:22-33.

Chung N, Y. Bogliotti, W. Ding, M. Vilarino, K. Takahashi, J. Chitwood, R. Schultz, and P.J. Ross. 2018. Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos. Epigenetics 16:1-9.

Daigneault B.W., S. Rajput, G.W. Smith, and P.J. Ross. 2018. Embryonic OCT4 is required for expanded bovine blastocyst formation. Sci. Rep. 8:7753.

da Silveira J.C., A.C.F.C.M. de Avila, H.L. Garrett, J. Bruemmer, Q. Winger, and G.J.  Bouma.  2017. Cell-secreted vesicles containing microRNAs as regulators of gamete maturation. J. Endocrinol. 236:R15-R27

Delgado, P.A., T.D. Lester, and R.W. Rorie.  2018.  Effect of a low-sodium, choline-based semen diluent on viability of bovine sperm stored at 4° C.  Adv. Reprod. Sci. 6:12-21.

Desaulniers, A.T., R.A. Cederberg, C.A. Lents, and B.R. White.  2017.  Expression and role of gonadotropin-releasing hormone 2 and its receptor in mammals.  Front. Endocrinol. 8:269.

Desaulniers, A.T., R.A. Cederberg, G.A. Mills, C.A. Lents, and B.R. White.  2017.  Production of a gonadotropin-releasing hormone 2 receptor knockdown (GnRHR2 KD) swine line.  Transgenic Res. 26:567-575.

Diaz, F.A., E.J. Gutierrez, E. Cramer, D.L. Paccamonti, G.T. Gentry, and K.R. Bondioli. 2018. Pregnancy rates following low-temperature storage of large equine embryos prior to vitrification. J. Equine Vet. Sci. 64:12-16.

Fan Z., M. Yang, M. Regouski, and I.A. Polejaeva. 2017. Effects of three different media on in vitro maturation and development, intracellular glutathione and reactive oxygen species levels, and maternal gene expression of abattoir-derived goat oocytes. Small Rumin. Res. 147:106-114.

Feltrin, C., L.H. de Aguiar, C.E.M. Calderón, I. de Sá Carneiro, F. de Jesus Moraes, J. da Silva Quetz., A.A.M. Lima, M.B. Wheeler, D. Rondina, J.L. Rodrigues, J.D. Murray, E.A. Maga, L.R. Bertolini, and M. Bertolini. 2017. Effects of oocyte source, cell origin, and embryo reconstruction procedures on in vitro and in vivo embryo survival after goat cloning. Anim. Reprod. 14:1110-1123.

Ferre L, J. Chitwood, C. Fresno, H. Ortega, M. Kjelland, and P.J. Ross. 2017. Effect of different mini-volume colloid centrifugation configurations on flow-cytometrically-sorted sperm recovery efficiency and quality using a computer-assisted semen analyzer. Reprod. Domest. Anim. 53:26-33.

de Lima L.F., M. Rubessa, R.M.P. Rocha, R. Winters, D.J. Milner, C.C. Campell, J.R. Figueiredo, and M.B. Wheeler. 2017. High diluted and dynamised follicle stimulating hormone modulates steroid production in isolated porcine preantral follicles cultured in vitro. Homeopathy 106:87-92.

de Lima, L.F., R.M.P. Rocha, A.B.G. Duarte, I.R. Brito, G.M. Silva, G.Q. Rodrigues, A.D. Sales, C.H. Lobo, A.A. Moura, M.B. Wheeler, A.P.R. Rodrigues, C.C. Campello, and J.R. Figueiredo. 2017. Unexpected effect of the vehicle of homeopathic FSH (grain ethanol) on the in vitro survival and development of isolated ovine preantral follicles. Microsc. Res. Tech. 80:406-418.

Geisert R.D., J.J. Whyte, A.E. Meyer, D.J. Mathew, M.C. Lucy, R.S. Prather, and T.E. Spencer. 2017. Rapid conceptus elongation in the pig: Interleukin 1 beta 2 and estrogen regulated phenomena. Mol. Reprod. Dev. 84:760-774.

Geisert R.D. and M.C. Lucy. 2018. Comparative Mammalian Female Reproduction-Pig. In: The Encyclopedia of Reproduction Vol 2., Chapter 91.

Geisert R.D., M.F. Smith, and J.A. Green. 2018. Utilizing a rat delayed implantation model to teach integrative endocrinology and reproductive biology. Adv. Physiol. Educ. 42:56-63.

Huang, D., L. Wang, J. Duan, C. Huang, X. Tian, M. Zhang, and Y. Tang. 2017. LIF-activated Jak signaling determines Esrrb expression during late-stage reprogramming. Biology Open. 7:29264.

Hughes, J.R., and T. Berger.  Regulation of apical blebbing in the porcine epididymis. J. Anat. 232:515-522.

Lents, C.L., J.F. Thorson, A.T. Desaulniers, and B.R. White.  2017.  RFamide-related peptide 3 and gonadotropin-releasing hormone-II are autocrine-paracrine regulators of testicular function in the boar. Mol. Reprod. Dev. 84:994-1003.

Lotti, S.N., K.M. Polkoff, M. Rubessa, and M.B. Wheeler. 2017. Modification of the genome of domestic animals. Anim. Biotechnol. 28:198-210.

Meyer, L.R., J.G. Powell, B.R. Kutz, M.L. Looper, A.H. Brown, Jr., and C.F. Rosenkrans, Jr.  2017. Associations of single nucleotide polymorphisms in the bovine prolactin gene with phenotypic traits in beef cattle. Agri. Gene. 5:7-11.

Nguyen, T.H., M.E. Kandel, M. Rubessa, M.B. Wheeler, and G. Popescu. 2017. Gradient light interference microscopy (GLIM) for 3D imaging of unlabeled specimens. Nat. Commun. 8:210.

Milner, D.J., M. Bionaz, E. Monaco, J.A. Cameron, J and M.B. Wheeler. 2018. Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue. Cell Tissue Res. 372:507-522.

Polkoff, K.M., and M.B. Wheeler. 2017. What’s coming: How biotechnology changes everything! 12th Simposium Internacional de Reproduction Animal Proceedings, pages 338-344.

Rocha, R.M.P., L.F. Lima, I.R. Brito, G.M. Silva, H.H.V. Correia, N.A. Ribeiro de Sá, A.C.A. Ferreira, A.D. Sales, C.H. Lobo, C.C. Campello, J. Smitz, M.B. Wheeler, and J.R. Figueiredo, 2017. Anti-Müllerian hormone (AMH) reduces growth rate without altering follicular survival in isolated caprine preantral follicles cultured in vitro. Reprod. Fertil. Dev. 29:1144-1154.

Rubessa, M., K. Polkoff, M. Bionaz, E. Monaco, D.J. Milner, S.J. Holllister, M.S. Goldwasser, and M.B. Wheeler. 2017. The use of the pig as a model for bone regeneration. Anim. Biotechnol. 28:275-287.

Rubessa, M., and M.B. Wheeler. 2017. Advancing bovine in vitro fertilization and embryo development: New analytics. 2017. 12th Simposium Internacional de Reproduction Animal Proceedings, pages 316-323, Cordoba, AR.

Rutigliano H.M., A. Wilhelm, J. Hall, B. Shi, Q. Meng, R. Stott, T.D. Bunch, K.L. White, C.J. Davies, and I.A. Polejaeva. 2017. Cytokine gene expression in the maternal-fetal interface in somatic cell nuclear transfer pregnancies in small ruminants. Reprod. Fertil. Dev. 29:646-657.

Silva, G.M., I.R. Brito, A.D. Sales, F.L.N. Aguia, A.B.G. Duarte, V.R. Araújo, L.A. Vieira, D.M. Magalhães-Padilha, L.F. Lima, B.G. Alves, L.B.R. Silveira, E.G. Lo Turco, A.P. Rodrigues, C.C. Campello, M.B. Wheeler, and J.R. Figueiredo. 2017. In vitro growth and maturation of isolated caprine preantral follicles: influence of insulin and FSH concentration, culture dish, co-culture and oocyte size on meiotic resumption. Theriogenology. 90:32-41.

Silva, R.F., I.R. Brito, L.F. Lima, F.L.N. Aguiar, G.Q. Rodrigues, I.L.C. Nascimento, R.M.P. Rocha, F.W.S. Cibin, M.B. Wheeler, C.C. Campello, A.P.R. Rodrigues, and J.R. Figueiredo. 2017. Platelet-derived growth factor-BB (PDGF-BB) improved in a concentration-dependent manner follicular survival as well as oocyte and follicular diameters after in vitro culture of goat preantral follicle enclosed in ovarian fragments. Anim. Reprod. 14:1095-1102.

Smith M.F., R.D. Geisert, and J.J. Parrish. 2018. Reproduction in domestic ruminants during the past 50 years: Discovery to application. J. Anim. Sci. 96:2952-2970.

Tribulo, P., J.I. Moss, M. Ozawa, Z. Jiang, X.C. Tian, and P.J. Hansen. 2017. WNT regulation of embryonic development likely involves pathways independent of nuclear CTNNB1. Reproduction. 153:405-419.

Vilarino M, S.T. Rashid, F.P. Suchy, B.R. McNabb, T. van der Meulen, E.J. Fine, S. Ahsan, N. Mursaliyev, V. Sebastiano, S.S. Diab, M.O. Huising, H. Nakauchi, and P.J. Ross. 2017 CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep. Sci. Rep. 7:17472

Wang, L., D. Huang, Z. Jiang, Y. Luo, C. Norris, M. Zhang, X. Tian, and Y. Tang. 2017. Akt3 is responsible for the survival and proliferation of embryonic stem cells. Biol. Open. 6:850-861.

Wang, L., D.L. Huang, C. Huang, Y.X. Yin, K. Vali, M. Zhang, and Y. Tang. 2017. Enhanced human somatic cell reprogramming efficiency by fusion of the MYC transactivation domain and OCT4. Stem Cell Res. 25:88-97.

Wang, L., J. Zongliang, D. Huang, J. Duan, C. Huang, S. Sullivan, K. Vali, Y. Yin, M. Zhang, J. Wegrzyn, X.C. Tian, and Y. Tang. 2018. Jak/Stat3 regulated global gene expression dynamics during late-stage reprogramming process. BMC Genomics. 19:183.

Weisgerber, D.W., D.J. Milner, H. Lopez-Lake, M. Rubessa, S. Lotti, K.M. Polkoff, R.A. Hortensius, C.L. Flanagan, S.J. Hollister, M.B. Wheeler, and B.A.C. Harley. 2017. A mineralized collagen-polycaprolactone composite promotes healing of a porcine mandibular ramus defect. Tissue Eng. Part A. 24:943-954.

Wheeler, M. B., and M. Rubessa. 2017. Integration of microfluidics in animal in vitro embryo production. Mol. Hum. Reprod. 23:248–256.

Whyte W.W., A.E. Meyer, L.D. Spate, J.A. Benne, R. Cecil, M.S. Samuel, C.N. Murphy, R.S. Prather, and R.D. Geisert. 2018. Inactivation of porcine interleukin-1β (IL1B2) results in failure of rapid conceptus elongation. Proc. Natl. Acad. Sci. 115:307-312.

Xu, W., B.A. Foster, M. Richards, K.R. Bondioli, G. Shah, and C.C. Green. 2018. Characterization of prostate cancer cell progression in zebrafish xenograft model. Int. J. Oncol. 52:252-260.


Bai Y, Zhu C, Feng M, Wei H, Li L, Zhao Z, Liu S, Ma N, Zhang X, Shi R, Fu C, Tian X, Wu Z, Zhang S 2018. Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells. Stem Cell Research & Therapy 9:200; https://doi.org/10.1186/s13287-018-0931-0.

Behluli B, Musliu A, Sherifi K, Youngs CR, and Rexhepi A 2017. Risk factors for occurrence of displaced abomasum and their relation to nutritional management of Holstein dairy cattle. Veterinarski Arhiv 87(4):419-430. DOI: 10.24099/vet.arhiv.a60216

Benaissa MH, S Ansel, A Mohamed-Cherif, K Benfodil, D Khelef, CR Youngs, R Kaidi, and K Ait-Oudhia. 2017. Seroprevalence and risk factors for Coxiella burnetii, the causative agent of Q fever in the dromedary camel (Camelus dromedarius) population in Algeria. Onderstepoort Journal of Veterinary Research 84(1):a1461. https://doi.org/10.4102/ojvr.v84i1.1461

Benaissa MH, Faye B, Youngs CR, and Kaidi R  2016.  Slaughterhouse survey of culled female camels (Camelus dromedaries) in southeast Algeria: Fetal wastage and pregnancy characteristics. Emirates Journal of Food and Agriculture 28(11):805-812. 10.9755/ejfa.2016-06-735

Correia, H.H.V., Vieira, L.A., Mielgo, C.M., Paes, V.M., Alves, B.G., Viana, J.R.,

Wheeler, M.B., Rodrigues, A.P.R., Figueiredo, J.R. 2018. Cilostamide affects in a concentration and exposure time-dependent manner the viability and the kinetics of in vitro maturation of caprine and bovine oocytes. Research in Veterinary Science 2018 Nov 12;122:22-28. doi: 10.1016/j.rvsc.2018.11.002.

CR Youngs 2018. Embryo transfer technologies in pigs In (H. Niemann and C. Wryzenski, Eds.) Animal Biotechnology 1: Reproductive Biotechnologies, Springer, pp 167- 178.

Daigneault BW, Vilarino M, Rajput S, Frum  T, Smith GW, Ross PJ 2018. CRISPR editing validation, immunostaining and DNA sequencing of individual fixed bovine embryos. Biotechniques 65(5):281-283. doi: 10.2144/btn-2018-0051.

Degefa T, A Lemma, E Demissie, S Ali, A Funga, and CR Youngs 2018. Superovulation response and in vivo embryo production potential of Boran cows in Ethiopia. Ethiop. J. Agric. Sci. 28(1):71-80.

Degefa T, Lemma A, Jemal J, Mamo G, Tegegne A, and Youngs CR 2016.  Ovarian follicular dynamics in purebred and crossbred cows in Ethiopia. African Journal of Biotechnology 15(33):1763-1770. DOI: 10.5897/AJB2016.15267

Degefa T, Lemma A, Tegegne A, and Youngs CR 2016.  Effects of genotype and FSH dose on estrous and ovarian response of Boran and Boran X Holstein Friesian cows in Ethiopia. Livestock Research for Rural Development Vol. 28, article 169. http://www.lrrd.org/lrrd28/9/dege28169.html

Duan JE, Flock K, Jue N, Zhang M, Jones A, Seesi S, Mandoiu I, Pillai S, Hoffman M, O’Neill R, Zinn S, Govoni K, Reed S, Jiang H, Jiang Z, Tian XC 2018. Dosage compensation and gene expression of the X chromosome in sheep. G3:Genes, Genomes, Genetics. 9(1):305-314. doi: 10.1534/g3.118.200815.

Duan JE, Zhang M, Flock K, Seesi SA, Mandoiu I, Jones A, Johnson E, Pillai S, Hoffman M, McFadden K, Jiang H, Reed S, Govoni K, Zinn S, Jiang Z, Tian XC 2018. Effects of Maternal Nutrition on the Expression of Genomic Imprinted Genes in Ovine Fetuses. Epigenetics 13(8):793-807. doi: 10.1080/15592294.2018.1503489.

Duan J, Zhu L, Dong H, Zheng X, Jiang Z, Chen J, Tian XC 2019. Analysis of mRNA abundance for histone variants, histone- and DNA-modifiers in bovine in vivo and in vitro oocytes and embryos. Sci Rep 9(1):217. doi: 10.1038/s41598-018-38083-4.

Duan JE, Shi W, Jue N, Jiang Z, Kuo L, O’Neill R, Wolf E, Dong H, Zheng X, Chen J, Tian XC 2019. Dosage Compensation of the X Chromosomes in Bovine Germline, Early Embryos and Somatic Tissues. Genome Biology and Evolution 11(1):242-252. doi: 10.1093/gbe/evy270.

Fan, Z., Perisse, I. V., Cotton, C. U., Regouski, M., Meng, Q., Domb, C., Van Wettere, A., Wang, Z., Harris, A., White, K. L., & Polejaeva, I. 2018. A sheep model of cystic fibrosis generated by CRISPR/Cas9 disruption of the CFTR gene. JCI insight, 3(19).

Huang D, Wang L, Talbot NC, Huang C, Pu L, Zhao X, Tian X, Zhang M, Tang Y 2018. Analyzing bovine OCT4 and NANOG enhancer activity in pluripotent stem cells using fluorescent protein reporters. PLoS ONE 13(10):e0203923. doi: 10.1371/journal.pone.0203923. eCollection 2018.

Jeckel KM, Boyarko AC, Bouma GJ, Winger QA, Anthony RV 2018. Chorionic somatomammotropin impacts early fetal growth and placental gene expression. J Endocrinol. 237(3):301-310.

Jiang Z, Lin J, Dong H, Zheng X, Marjani SL, Duan JE, Ouyang Z, Chen J, and Tian XC 2018. DNA methylomes of bovine gametes and in vivo produced preimplantation embryos. Biol Reprod. 99(5):949-959. doi: 10.1093/biolre/ioy138

Koroghli, J., Floyd, E., Regouski, M., Rood, K., Gash, K., Panter, K., Stott, R. D., Davies, C., Polejaeva, I., & Rutigliano, H 2018. Gene expression and lymphocyte population at the fetal-maternal interface in sheep pregnancies established by somatic cell nuclear transfer. Reproduction, Fertility and Development. doi: 10.1071/RD17224.

Liu, L., Kandel, M., Rubessa, M., Schreiber, S., Wheeler, M.B., Popescu, G. 2018. Topography and refractometry of sperm cells using SLIM. Journal of Biomedical Optics 23(2):1-6. doi: 10.1117/1.JBO.23.2.025003.

Manca, S., B. Upadhyaya, E. Mutai, A.T. Desaulniers, R.A. Cederberg, B.R. White and J. Zempleni. 2018. Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns. Sci. Rep. 8:11321.

McWhorter ES, Russ JE, Winger QA, Bouma GJ. 2018. Androgen and estrogen receptors in placental physiology and dysfunction. Front Biol 13(5):315-326.

Milner, D. J., M. Bionaz, E. Monaco, J. A. Cameron and M. B. Wheeler. 2018. Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue. Cell and Tissue Research 372(3): 507-522.

Morrison, R.J., Nasser, H.B., Kashlan, K.N., Zopf, D.A., Flanangan, C.L., Wheeler, M.B., Green, G.E., Hollister, S.J. 2018. Co-Culture of Adipose-Derived Stem Cells and Chondrocytes on Three-Dimensionally Printed Bioscaffolds for Craniofacial Cartilage Engineering. Laryngoscope 128:E251-E257.

Page, R., T. Lester, R. Rorie, and C. Rosenkrans, Jr.  2018.  Ergot Alkaloid Effects on Bovine Sperm Motility In Vitro.  Adv. Reprod. Sci. (accepted).

Ross PJ, Sampaio RV 2018. Epigenetic remodeling in preimplantation embryos: cows are not big mice. Animal Reproduction 15(3):204-2014. doi: 10.21451/1984-3143-AR2018-0068

Rubessa, M., Ambrosi, A., Gonzalez-Pena, D., Polkoff, K.M., Wheeler, M.B.  2018. Non-Invasive Nuclear Magnetic Resonance Analysis of Male and Female Embryo Metabolites During In Vitro Embryo Culture. Metabolomics 14: 113. https://doi.org/10.1007/s11306-018-1414-0.

Rubessa, M., Lotti, S.N., Kandel, M.E., Popescu, G., Wheeler, M.B. 2018. SLIM Microscopy allows for visualization of DNA-containing liposomes designed for sperm-mediated gene transfer in cattle. Molecular Biology Report. doi: 10.1007/s11033-018-4525-9. [Epub ahead of print]

Vilarino M, Rashid ST, Suchy FP, Lindsay H, Reyes J, McNabb BR, van der Meulen T, Huising MO, Nakauchi H, Ross PJ 2018. Mosaicism diminishes the value of pre-implantation embryo biopsies for detecting CRISPR/Cas9 induced mutations in sheep. Transgenic Research. doi: 10.1007/s11248-018-0094-x.

Wang T, Babayev E, Jiang Z, Li G, Zhang M, Esencan E, Horvath T, Seli E 2018. Mitochondrial unfolded protein response gene Clpp is required to maintain ovarian follicular reserve during aging, for oocyte competence, and development of pre-implantation embryos. Aging Cell. e12784. PMID: 29851234

West RC, McWhorter ES, Ali A, Goetzman LN, Russ JE, Anthony RV, Bouma GJ, Winger QA 2018. HMGA2 is regulated by LIN28 and BRCA1 in human placental cells. Biol Repod 100(1):227-238. doi: 10.1093/biolre/ioy183. [Epub ahead of print]

West RC, Bouma GJ, Winger QA. 2018. Shifting perspectives from "oncogenic" to oncofetal proteins; how these factors drive placental development. Reprod Biol Endocrinol 16(1):101.

Yang, M., Perisse, I., Fan, Z., Regouski, M., Meyer-Ficca, M., & Polejaeva, I 2018. Increased pregnancy losses following serial somatic cell nuclear transfer in goats. Reproduction, Fertility and Development. Doi: 10.1071/RD17323.

Yugo DM, Heffron CL, Ryu J, Uh K, Subramaniam S, Matzinger SR, Overend C, Cao D, Kenney SP, Sooryanarain H, Cecere T, LeRoith T, Yuan L, Jue N, Clark-Deener S, Lee K, Meng XJ 2018. Infection dynamics of hepatitis E virus in wild-type and immunoglobulin heavy chain knockout JH (-/-) gnotobiotic piglets. J Virol. pii: JVI.01208-18. doi: 10.1128/JVI.01208-18.

Zhou, G., Wei, H., Wang, X., Yang, M., Bunch, T. D., Polejaeva, I., White, K. L., Wang, Z., & Meng, Q. 2018. Serial Culture Is Critical for In Vitro Development of Parthenogenetic Embryos in the Golden Syrian Hamster. Cellular reprogramming 20(3):187-195. doi: 10.1089/cell.2017.0070

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