NC_old1180: Control of Endemic, Emerging and Re-emerging Poultry Respiratory Diseases in the United States

(Multistate Research Project)

Status: Inactive/Terminating

Project Menu

SAES-422 Reports

12/01/2014

01/11/2016

12/16/2016

12/08/2017

01/01/1970

Publications:

-S. Farjana, F. Eldemery, R.A. Zegpi, S.L. Gulley, A. Mishra, V.L. van Santen, H. Toro. Early

vaccination of chickens induces suboptimal immunity against infectious bronchitis virus. Avian

Diseases (submitted for publication August 2018).

-Zegpi, R.A., A. Mishra, S. Gulley, V.L. van Santen, K.S. Joiner, H. Toro. Enhanced cross

protection to infectious bronchitis viruses upon vaccination postponed beyond day one of age.

Avian Diseases (submitted for publication October 2018).

-Da Silva A.P., R. Hauck, C. Kern, Y. Wang, H. Zhou, R.A. Gallardo. Susceptibility of

chickens with different MHC haplotypes to infectious bronchitis virus genotypes. 2018. Avian

Dis. Submitted.

-Saelao P., Y. Wang. R. A. Gallardo, S. J. Lamont, J. M. Dekkers, T. Kelly, H. Zhou. Novel

insights into the host immune response of chicken Harderian gland tissue, during Newcastle

disease virus infection and heat treatment. 2018. BMC Vet. Res. In Press.

-Cadena, M., M. Hoffman, R. A. Gallardo, A. Figueroa, M. Lubell, M. Pitesky. Using Social

Network Analysis to Characterize the Collaboration Network of Backyard Poultry Trainers in

California. 2018. Prev. Vet. Med. 158: 129-136 https://doi.org/10.1016/j.prevetmed.2018.07.016

-Rowland K., A. Wolc, R.A. Gallardo, T. Kelly, H. Zhou, J. Dekkers, S. J. Lamont. Genome-

Wide Association Study of a Commercial Egg Laying Line Challenged with Newcastle Disease

Virus. 2018. Frontiers Genetics.

- Kayang, B.B., Amuzu-Aweh, N., Botchway, P.K., Tudeka, C.K., Naazie, A., Aning, K.G.,

Dekkers, J., Lamont, S.J., Gallardo, R., Kelly, T.R., Bunn, D., & Zhou, H. (2018). Performance of three local chicken ecotypes of Ghana naturally exposed to velogenic Newcastle disease virus.

Proceedings of the World Congress on Genetics Applied to Livestock Production, 11.846.

- Botchway, P.K., Amuzu-Aweh, N., Naazie, A., Aning, K.G., Zhou, H., Dekkers, J., Lamont, S.J., Gallardo, R., Kelly, T.R., Bunn, D., & Kayang, B.B. (2018). Genotypic and phenotypic

characterisation of three local chicken ecotypes of Ghana based on principal component analysis

and body measurements. Proceedings of the World Congress on Genetics Applied to Livestock

Production, 11.812.

- Amuzu-Aweh, N., Kayang, B.B., Muhairwa, A.P., Botchway, P.K., Naazie, A., Aning, K.G.,

Gallardo, R., Kelly, T.R., Zhou, H., Lamont, S.J., & Dekkers, J.C.M. (2018). Genetic parameters

and genomic regions associated with growth rate and response to Newcastle disease in local

chicken ecotypes in Ghana and Tanzania. Proceedings of the World Congress on Genetics Applied to Livestock Production, 11.774.

-Mushi, J.R., Muhairwa A. P. G.C. Chieanga. P. M Msoffe, Dekkers, J., Lamont, S.J., Gallardo,

R., Kelly, T.R., Bunn, D., & Zhou, H. (2018).Phenotypic and genotypic diversity of selected Free Range Local Chickens in Tanzania. Proceedings of the World Congress on Genetics Applied to Livestock Production, 11.812.

- Muhairwa A. P. G.C. Chieanga. J.R. Mushi, P. M Msoffe, N. Amuzu-Aweh, M. Walugembe,

Dekkers, J., Lamont, S.J., Gallardo, R., Kelly, T.R., & Zhou, H. (2018). Investigation of Genetic

Resistance to Newcastle Disease in Local Chickens in Tanzania using Natural Challenge by Field Velogenic NDV Strains. Proceedings of the World Congress on Genetics Applied to Livestock Production, 11.812.

-Aleuy O.A., M. Pitesky, R. A. Gallardo. Using Multinomial and Space -Time Permutation

Models to Understand the Epidemiology of Infectious Bronchitis in California Between 2008

and 2012. Avian Dis. 2018. 62:2. 226-232.

-Deist M.S., D.A. Bunn, T.R. Kelly, J.C.M. Dekkers, H. Zhou, S.J. Lamont. Novel Analysis of

the Harderian Gland Transcriptome response to Newcastle Disease Virus in two Inbred Chicken

Lines. Sci. Reports. 2018.

-Zhang J., M. Kaiser, Deist M.S., R. A. Gallardo, D.A. Bunn, T.R. Kelly, J.C.M. Dekkers, H.

Zhou, S.J. Lamont. Transcriptome Analysis in Spleen Reveals Differential Regulation of Response to Newcastle Disease Virus in Two Chicken Lines. Sci. Rep. 2018. 8:1278. DOI:10.1038/s41598-018-19754-8

-Wilson B. K, T. Kelly, D.A. Bunn, and H. Zhou. 2018. Key criteria and models for implementing a sustainable chicken breeding and distribution program for smallholder poultry producers. Livestock Research for Rural Development. 30 (4), Article #67. Retrieved April 13, 2018, http://www.lrrd.org/lrrd30/4/wils30067.html.

- Wang, Y., P. Saelao, K. Chanthavixay, R. A. Gallardo, D. Bunn, S. Lamont, J. Dekkers, T.

Kelly, H. Zhou. Physiological Responses to Heat Stress in Two Genetically Distinct Chicken

Inbred Lines. Poultry Science. 2017. https://doi.org/10.3382/ps/pex363

-Deist M.S., R. A. Gallardo, D.A. Bunn, T.R. Kelly, J.C.M. Dekkers, H. Zhou, S.J. Lamont.

Resistant and susceptible chicken lines show distinctive responses to Newcastle disease virus

infection in the lung transcriptome. BMC Genomics. 18:989. 2017. https://doi.org/10.1186/s12864-017-4380-4

-Derksen T.J., Lampron R., Hauck R., M. Pitesky, R. A. Gallardo. Biosecurity Assessment and

Seroprevalence of Respiratory Diseases in Backyard Poultry Flocks Located Close and Far from

Commercial Premises. Avian Diseases. 2017. 62:1 1-5.

http://www.aaapjournals.info/doi/pdf/10.1637/11672-050917-Reg.1

-Da Silva A.P., Hauck R., H. Zhou, R. A. Gallardo, Understanding Immune Resistance to

Infectious Bronchitis Using Major Histocompatibility Complex Chicken Lines. Avian Diseases.

61(3):358-365. 2017.

-Deist M.S., R. A. Gallardo, D.A. Bunn, T.R. Kelly, J.C.M. Dekkers, H. Zhou, S.J. Lamont. NovelMechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines Following Infection with Newcastle Disease Virus. Clinical and vaccine immunology. 2017. http://cvi.asm.org/content/24/5/e00027-17.short

-Li, J., Zeinab H. Helal, Brian Ladman, Christopher Karch, Jack Gelb Jr, Peter Burkhard and

Mazhar I. Khan. Nanoparticle vaccine for avian influenza virus: A challenge study against

highly pathogenic H5N2 subtype. J Virol Antivir Res. 6:4:1-5: doi: 10.4172/2324-8955.

December 30, 2017.

-Ebrahimi-Nik, H., M. R. Bassami, M. Mohri, M. Rad and M. I. Khan. Bacterial ghost of

avian pathogenic E. coli (APEC) serotype O78:K80 as a homologous vaccine against avian

colibacillosis. PLOS-one: 1-16: doi.org/10.1371/journal.pone.0194888. March 22, 2018.

-Li J, Helal ZH, Karch CP, Mishra N, Girshick T, Garmendia A, et al. (2018). A selfadjuvanted

nanoparticle based vaccine against infectious bronchitis virus. PLoS ONE 13(9):

e0203771. https://doi.org/10.1371/journal. pone.0203771

-Li, J., Z. H. Helal and M.I. Khan. Self-assembling protein nanoparticles open a new avenue

for next generation veterinary vaccines. J Nanomed Res. 7: (2), 82-84. 2018.

-Li J, Helal Z, Ladman B, Karch C, Gelb J, et al. (2018) Nanoparticle Vaccine for Avian

Influenza Virus: A Challenge Study against Highly Pathogenic H5N2 Subtype. J Virol

Antivir Res 7:1. doi: 10.4172/2324-8955.1000179

-Ponnuraj NP, Tien YT, Vega Rodriguez W, Krieter A, and Jarosinski, KW. The Herpesviridae conserved multifunctional infected cell protein 27 (ICP27) is important, but not required for replication and oncogenicity of Marek’s disease alphaherpesvirus (MDV). JVI (in revisions).

-Jang H, Elaish M, Kc M, Abundo MC, Ghorbani A, Ngunjiri JM, Lee CW. Efficacy and synergy of

live-attenuated and inactivated influenza vaccines in young chickens. PLoS One.

13(4):e0195285. 2018.

-Ghorbani A, Ngunjiri JM, Xia M, Elaish M, Jang H, KC M, Abundo MC, Jiang X, Lee CW.

Heterosubtypic protection by live attenuated and chimeric norovirus P-particle-M2e vaccines in

chickens. Submitted.

-Lee, D., Bertran, K., Kwon, J., Swayne, D.E. 2017. Evolution, global spread, and pathogenicity of highly pathogenic avian influenza H5Nx clade 2.3.4.4. Journal of Veterinary Science. 18(S1):269-280. https://doi.org/10.4142/jvs.2017.18.S1.269.

-Kapczynski, D.R., Sylte, M.J., Killian, M.L., Torchetti, M.K., Chrzastek, K., Suarez, D.L. 2017. Protection of commercial turkeys following inactivated or recombinant H5 vaccine application against the 2015 U.S. H5N2 clade 2.3.4.4 highly pathogenic avian influenza virus. Veterinary Immunology and Immunopathology. 191(2017):74-79. http://dx.doi.org/10.1016/j.vetimm.2017.08.001.

-Bertran, K., Balzli, C.L., Lee, D., Suarez, D.L., Kapczynski, D.R., Swayne, D.E. 2017. Protection of White Leghorn chickens by U.S. emergency H5 vaccines against clade 2.3.4.4 H5N2 high pathogenicity avian influenza virus. Vaccine. 35:6336-6344.

http://dx.doi.org/10.1016/j.vaccine.2017.05.051.

-Santos, J., Obadan, A., Cardenas Garcia, S., Carnaccini, S., Pantin Jackwood, M.J., Suarez, D.L., Kapczynski, D.R., Perez, D.R. 2017. Short- and long-term protective efficacy against clade 2.3.4.4 H5N2 highly pathogenic avian influenza virus following primeboost vaccination in turkeys. Vaccine. 35:5637-5643. http://dx.doi.org/10.1016/j.vaccine.2017.08.059.

-Stephens, C., Spackman, E. 2017. Thermal inactivation of avian influenza virus in poultry litter as a method to decontaminatepoultry houses. Preventive Veterinary Medicine. 145:73–77. https://doi.org/10.1016/jpreventmed.2017.06.012.

-Bertran, K., Swayne, D.E., Pantin Jackwood, M.J., Kapczynski, D.R., Spackman, E., Suarez, D.L. 2016. Lack of chicken adaptation of newly emergent Eurasian H5N8 and reassortant H5N2 high pathogenicity avian influenza viruses in the U.S. is consistent with restricted poultry outbreaks in the Pacific flyway during 2014-2015. Virology. 494:190-197.

https://doi.org/10.1016/j.virol.2016.04.019.

-Lee, D., Bahl, J., Torchetti, M.K., Killian, M.L., Ip, H.S., Swayne, D.E. 2016. Highly pathogenic avian influenza virus and generation of novel reassortants, United States, 2014-2015. Emerging Infectious Diseases. 22(7):1283-1285.

http://dx.doi.org/10.3201/eid2207.160048.

-Reeves, A.B., Poulson, R.L., Muzyka, D., Ogawa, H., Imai, K., Bui, V., Hall, J.S., Pantin Jackwood, M.J., Stallknecht, D.E., Ramey, A.M. 2016. Limited evidence of intercontinental dispersal of avian paramyxovirus serotype 4 by migratory birds. Infection, Genetics and Evolution. 40:104-108. http://dx.doi.org/10.1016/j.meegid.2016.02.031.

-Dimitrov, K.M., Zarkov, I.S., Dinev, I., Goujgoulova, G.V., Miller, P.J., Suarez, D.L. 2016. Histopathological characterization and shedding dynamics of guineafowl (Numida meleagris) intravenously infected with a H6N2 low pathogenicity avian influenza

virus. Avian Diseases. 60(1s):279-85. https://doi.org/10.1637/11141-050815-Reg.

-Xu, Y., Bailey, E., Spackman, E., Li, T., Wang, H., Long, L., Baroch, J.A., Cunningham, F.L., Lin, X., Jarman, R.G., Deliberto, T.J., Wan, X. 2016. Limited antigenic diversity in contemporary H7 avian-origin influenza A viruses from North America. Scientific Reports. 6:20688. http://doi.org/10.1038/srep20688.

-Sims, L., Swayne, D.E. 2016. Avian influenza control strategies. In: Swayne, D.E., editor. Animal Influenza. 2nd edition. Ames, IA: Wiley- Blackwell. p.363-377. Swayne, D.E., Kapczynski, D.R. 2016. Vaccines and vaccination for avian influenza in poultry. In: Swayne, D.E., editor. Animal Influenza. 2nd edition. Ames, IA: Wiley- Blackwell. p.378-434.

-Chrzastek, K., Lee, D., Gharaineh, S., Zsak, A., Kapczynski, D.R. 2018. Characterization of H9N2 avian influenza viruses from the Middle East demonstrates heterogeneity at amino acid position 226 in the hemagglutinin and potential for transmission to

mammals. Virology. 518: 195-2017. https://doi.org/10.1016/j.virol.2018.02.016.

-Spackman, E. 2018. Reovirus infections. In: Saif, Y.M., Toro, H., editors. Diagnosis of Major Poultry Diseases. Zaragoza, Spain: Editorial Servet. P. 35-37.

-Bertran, K., Balzli, C.L., Kwon, Y., Tumpey, T.M., Clark, A., Swayne, D.E. 2017. Airborne transmission of highly pathogenic influenza virus during processing of infected poultry. Emerging Infectious Diseases. 23(11):1806-1814.

https://doi.org/10.3201/eid2311.170672.

-Kapczynski, D.R., Pantin Jackwood, M.J., Spackman, E., Chrzastek, K., Suarez, D.L., Swayne, D.E. 2017. Homologous and

heterologous antigenic matched vaccines containing different H5 hemagglutinins provides variable protection of chickens from

the 2014 U.S. H5N8 and H5N2 clade 2.3.4.4 highly pathogenic avian influenza viruses. Vaccine. 35:6345-6353.

http://dx.doi.org/10.1016/j.vaccine.2017.04.042.

-Bertran, K., Lee, D., Pantin Jackwood, M.J., Spackman, E., Balzli, C.L., Suarez, D.L., Swayne, D.E. 2017. Pathobiology of clade 2.3.4.4 H5Nx high pathogenicity avian influenza virus infections in minor gallinaceous poultry supports early backyard flock introductions in Western U.S., 2014-2015. Journal of Virology. 91(21):e00960-17. https://doi.org/10.1128/JVI.00960-17.

-Chen,L., Lee, D., Liu, Y., Li, W., Swayne, D.E., Chang, J., Chen, Y., Lee, F., Tu, W., Lin, Y. 2018. Reassortant clade 2.3.4.4 of highly pathogenic avian influenza A (H5N6) virus, Taiwan, 2017. Emerging Infectious Diseases. 24(6):1147-1149.

https://dx.doi.org/10.3201/eid2406.172071.

-Balzli, C.L., Bertran, K., Lee, D., Killmaster, L.F., Pritchard, N., Linz, P., Mebastsion, T., Swayne, D.E. 2018. The efficacy of

recombinant turkey herpesvirus vaccines targeting the H5 of highly pathogenic avian influenza virus from the 2014/2015 North

American outbreak. Vaccine. 36:84-90. https://doi.org/10.1016/j.vaccine.2017.11.026.

-Ramey, A.M., Deliberto, T.J., Berhane, Y., Swayne, D.E., Stallknecht, D.E. 2018. Lessons learned from research and surveillance directed at highly pathogenic influenza A viruses in wild birds inhabiting North America. Virology. 518:55-63.

https://doi.org/10.1016/j.virol.2018.02.002.

-Muzyka, D., Pantin Jackwood, M.J., Starick, E., Fereidouni, S. 2015. Evidence for genetic variation of Eurasian avian influenza

viruses, subtype H15: The first report of an H15N7 virus. Archives of Virology. 161(3):605-612. https://doi.org/10.1007/s00705-

015-2629-2.

-Spackman, E. 2018. Newcastle disease and other avian paramyxoviruses. In: Saif, Y.M., Toro, H., editors. Diagnosis of Major Poultry Diseases. Zaragoza, Spain: Editorial Servet. P. 7-10.

-Liu, Y., Lee, D., Chen, L., Lin, Y., Li, W., Hu, S., Chen, Y., Swayne, D.E., Lee, M. 2018. Detection of reassortant H5N6 clade 2.3.4.4 highly pathogenic avian influenza virus in a black-faced spoonbill (Platalea minor) found dead, Taiwan, 2017. Infection, Genetics and Evolution. 62:275-278. https://doi.org/10.1016/j.meegid.2018.04.026.

-Lee Dong-Hun, Torchetti, M.K., Killian, M.L., Berhane, Y., Swayne, D.E. 2017. Highly pathogenic avian influenza A(H7N9) virus, Tennessee, USA, March 2017. Emerging Infectious Diseases. 23(11):1860-1863. https://dx.doi.org/10.3201/eid2311.171013.

-Dimitrov, K. M., Afonso, C. L., Yu, Q., Miller, P. J. Newcastle disease vaccines—A solved problem or a continuous challenge?

Veterinary Microbiology, 206:126-136. 2017. http://dx.doi.org/10.1016/j.vetmic.2016.12.019. Log No. 334453.

-Ji, Y., Liu, T., Jia, Y., Liu, B., Yu, Q., Cui, X., Guo, F., Chang, H., Zhu, Q. Two single mutations in the fusion protein of Newcastle disease virus confer hemagglutinin-neuraminidase independent fusion promotion and attenuate the pathogenicity in chickens. Virology, 509:146-151. 2017. doi: 10.1016/j.virol.2017.06.021.

-Hu, H., Roth, J., Zsak, L., Yu. Q. Engineered Newcastle disease virus expressing the F and G proteins of aMPV-C confers protection against challenges in turkeys. Scientific Reports, 2017 Jun 22;7(1):4025. doi: 10.1038/s41598-017-04267-7. Log No. 338700.

-Eldemery, F., Li, Y., Yu, Q., van Santen, V., Toro, H. Infectious bronchitis virus S2 of 4/91 expressed from recombinant virus does not protect against Ark-type challenge. Avian Diseases, 61(3):397-401, 2017.

-Yu, Q. Metapneumovirus infections. In Diagnosis of Magior Poultry Diseases, pp. 38-40. Edited by Y. M. Saif & H. Toro. Zaragoza, Spain: Servet. 2017.

-Ji, Y., Tao Liu, T., Du, Y., Cui, X., Yu, Q., Wang, Z., Zhang, J., Li, Y., Zhu, Q. A novel genotype VII Newcastle disease virus vaccine candidate generated by mutation in the L and F genes confers improved protection in chickens. Veterinary Microbiology,

216:99-106. 2018. https://doi.org/10.1016/j.vetmic.2018.01.021.

-Butt, S. L., Taylor, T. L., Volkening, J., Dimitrov, K. M., Williams-Coplin, D., Lahmers, K. K., Rana, A. M., Suarez. C. L., Afonso, C. L., Stanton, J. B., 2018. Rapid and sensitive virulence prediction and identification of Newcastle disease virus genotypes using third-generation sequencing. Virology Journal, Accepted manuscript.

-He, Y., Taylor, T. L., Dimitrov, K. M.*, Butt, S. L., Stanton, J. B., Goraichuk, I. V., Fenton, H., Poulson, R., Zhang, J., Brown, C. C., Ip, H. S., Isidoro-Ayza, M., Afonso, C. L., 2018. Whole-genome sequencing of genotype VI Newcastle disease viruses from formalin fixed paraffin-embedded tissues from wild pigeons reveals continuous evolution and previously unrecognized genetic diversity in the U.S. Virology Journal. 15:9. https://doi.org10.1186/s12985-017-0914-2.

-Susta, L., Segovia, D., Olivier, T. L., Dimitrov, K. M., Shittu, I., Marcano, V., Miller, P. J. 2018. Newcastle Disease Virus Infection in Quail. Veterinary Pathology. 55, 682-692, https://doi.org/10.1177/0300985818767996.

-Dimitrov, K. M., Sharma, P., Volkening, J. D., Goraichuk, I. V., Wajid, A., Rehmani, S. F., Basharat, A., Shittu, I., Joannis, T. M., Miller, P. J., Afonso, C. L., 2017. A robust and cost-effective approach to sequence and analyze complete genomes of small RNA viruses. Virology Journal 14, 72. https://doi.org/10.1186/s12985-017-0741-5.

-Ramey, A. M., Goraichuk, I. V., Hicks, J.T., Dimitrov, K. M., Poulson, R. L., Stallknecht, D. E., Bahl, J., Afonso, C. L., 2017. Assessment of contemporary genetic diversity and inter-taxa/inter-region exchange of avian paramyxovirus serotype 1 in wild birds sampled in North America. Virology Journal 14, 43. https://doi.org/10.1186/s12985-017-0714-8.

-Sabra, M., Dimitrov, K. M., Goraichuk, I. V., Wajid, A., Sharma, P., Williams-Coplin, D., Basharat, A., Rehmani, S. F., Muzyka, D. V., Miller, P.J., Afonso, C. L., 2017. Phylogenetic assessment reveals continuous evolution and circulation of pigeon-derived virulent avian avulaviruses 1 in Eastern Europe, Asia, and Africa. BMC Veterinary Research 13, 291. https://doi.org/10.1186/s12917-017-1211-4.

-Wajid, A., Dimitrov, K. M., Wasim, M., Rehmani, S. F., Basharat, A., Bibi, T., Arif, S., Yaqub, T., Tayyab, M., Ababneh, M., Sharma, P., Afonso, C. L., 2017. Repeated isolation of virulent Newcastle disease viruses in poultry and captive non-poultry avian species in Pakistan from 2011 to 2016. Preventive Veterinary Medicine 142, 1-6. http://dx.doi.org/10.1016/j.prevetmed.2017.04.010.

-Taylor, T. L., Dimitrov, K. M., Afonso, C. L., 2017. Genome-wide analysis reveals class and gene specific codon usage adaptation in avian paramyxoviruses 1. Infection, genetics and evolution 50, 28-37. http://dx.doi.org/10.1016/j.meegid.2017.02.004.

-Taylor, T. L., Miller, P. J., Olivier, T. L., Montiel, E., Cardenas Garcia, S., Dimitrov, K. M., Williams-Coplin, D., Afonso, C. L., 2017. Repeated Challenge with Virulent Newcastle Disease Virus Does not Decrease the Efficacy of Vaccines. Avian Diseases, 61:245- 249. https://doi.org/10.1637/11555-120816-ResNote.1.

-Goraichuk, I. V., Dimitrov, K. M., Sharma, P., Miller, P. J., Swayne, D. E., Suarez, D. L., Afonso, C. L., 2017. Complete Genome Sequences of Four Avian Paramyxoviruses of Serotype 10 Isolated from Rockhopper Penguins on the Falkland Islands. Genome Announcements 5, e00472-00417. http://dx.doi.org/10.1128/genomeA.00472-17.

-Fuller, C., Londt, B., Dimitrov, K. M., Lewis, N., van Boheemen, S., Fouchier, R., Coven, F., Goujgoulova, G., Haddas, R., Brown, I., 2017. An Epizootiological Report of the Re-emergence and Spread of a Lineage of Virulent Newcastle Disease Virus into Eastern Europe. Transboundary and Emerging Diseases 64, 1001-1007. http://dx.doi.org/10.1111/tbed.12455.

-Vagnozzi, A. E., G. Beltran, G., Zavala, L. R. Read, S. Sharif, and M. García. Cytokine gene

transcription in the trachea, harderian gland, and trigeminal ganglia of chickens inoculated with

virulent infectious laryngotracheitis virus (ILTV) strain. Avian Pathology. doi.org/10.1080/03079457.2018.1492090. 2018.

-Loncoman, C. A., Hartley, C.A., Coppo, M.J.C., Browning, G.F., Beltran, G., Riblet, S., Devlin,

M. and García M. (2018). The snp genotyping assay shows that vaccination can limit the

number and diversity of recombinant progeny of infectious laryngotracheitis viruses from the

USA. Appl Environ Microbiol. doi: 10.1128/aem.01822-18

-García, M. and G. Zavala. Commercial vaccines and vaccination strategies against infectious

laryngotracheitis (ILT): What we have learned and knowledge gaps that remain. Submitted

Avian Diseases.

-Spatz, S. J., M. Garcia, S. M. Ribletb, T. A. Ross, J. D.Volkening, T. L. Taylor, T. Kim and C. L. Afonso. MinION sequencing to genotype US strains of Infectious laryngotracheitis virus.

Submitted Avian Pathology.

-Dunn, J. R., K. M. Dimitrov, P. J. Miller, M. García, A. Brown, A. Hartman. Evaluation of

protective efficacy when combining HVT vector vaccines. Submitted Avian Diseases.

-Aston, E.J., B.J Jordan, S.M. Williams, M. Garcia, M.W. Jackwood. Effect of pullet vaccination

development on development and longevity of immunity. Submitted 2018 Avian Diseases.

-Aston, E.J., M.W. Jackwood, R.M. Gogal Jr., D. J. Hurley, B.D. Fairchild, D.A. Hilt, S. Cheng,

L.R. Tensa, M. Garcia and B.J Jordan. Ambient ammonia does not inhibit the immune response

to infectious bronchitis virus vaccination and protection from homologous challenge in broiler

chickens. Submitted 2018 Veterinary Immunology and Immunopathology.

Presentations:

-Farjana, S., Eldemery, F., Zegpi, R.A., Gulley, S.L., Mishra, A., van Santen, V.L., H. Toro (2018) Early Vaccination of Chickens Induces Suboptimal Immunity to Infectious Bronchitis Virus. 2^nd International Avian Respiratory Disease Conference. Athens, GA.

-Van Santen, V., H. Toro (2018). Role of S1 N-terminal domain amino acid differences among

ArkDPI IBV vaccine subpopulations in differential binding to chicken tissues and selection in

chickens. 2nd International Avian Respiratory Disease Conference. Athens, GA.

-Zegpi, R., K. Joiner, A. Mishra, S. Gulley and H. Toro (2018). Age of Vaccination Influences

Infectious Bronchitis Virus Cross-protection. Annual Convention AVMA/AAAP, July 13-17,

Denver, CO.

-H. Toro Vicky van Santen, Kellye S. Joiner, Russell Cattley (2018). Early Vaccination Induces

Suboptimal Immunity against Avian Infectious Bronchitis Virus. Conference of Research Workers in Animal Diseases (CRWAD), Dec 1-4, Chicago, IL.

-Vicky L. van Santen, Fatma E. Eldemery, Kellye S. Joiner (2018). Role of S1 N-terminal Domain Amino Acid Differences Among ArkDPI IBV Vaccine Subpopulations in Differential Binding to Chicken Tissues and Selection in Chickens. AAAP meeting, Denver, CO

-G.C. Chiwanga, A. P. Muhairwa, J.R. Mushi, P.L.M Msoffe, R.A. Max, E.N. Amuzu-Aweh, J.

Dekkers, R. Gallardo, S.J. Lamont, T. R. Kelly, and H. Zhou. 2018. Viral clearance in Free-range local chicken in Tanzania following challenge by Lentogenic Newcastle Disease virus. Avian Immunology Research Group meeting, Oxford, UK.

-Princess K. Botchway, Esinam N. Amuzu-Aweh, Muhammed Walugembe, Augustine Naazie,

George K. Aning, Perot Saelao, Ying Wang, Huaijun Zhou, Jack Dekkers, Sue J. Lamont, Rodrigo Gallardo, Terra R. Kelly, and Boniface B. Kayang. Genome Wide Association Analysis for Response to Newcastle Disease Virus in Local Chicken Ecotypes of Ghana. Avian Immunology Research Group meeting, Oxford, UK.

-Mushi. J.R., A. P. Muhairwa, G.C. Chiwanga, P.L.M Msoffe, J. Dekkers, R. Gallardo, S.J.

Lamont, T. R. Kelly, and H. Zhou, M. Walugembe. 2018. Immunological phenotypes of Tanzanian free range local chickens (FRLC) to lentogenic Newcastle disease virus. Avian Immunology Research Group meeting, Oxford, UK.

-K. Rowland. H. Zhou, R. Gallardo, T. Kelly, A. Wolc. J. Dekkers, Lamont. S.J. 2018. Genomewide association study of complex traits in response to Newcastle disease virus in chickens. Poultry Science Annual Meeting, San Antonio, TX

-Karen Tracy, Perot Saelao, Ying Wang, Rodrigo A. Gallardo, Susan J. Lamont, and Huaijun

Zhou. 2018.Dynamics of antiviral response to Newcastle Disease Virus (NDV) and heat stress in

chicken spleen. Poultry Science Annual Meeting, San Antonio, TX

-Karen Tracy, Stephen Preston, Perot Saelao, Ying Wang, Rodrigo A. Gallardo, Susan J. Lamont, Adrian Smith, and Huaijun Zhou. 2018. Immune Repertoire Analysis and the Systemic Immune Response during Newcastle Disease Virus Infection in Chickens. Avian Immunology Research Group meeting, Oxford, UK

-Karen Tracy, Stephen Preston, Perot Saelao, Ying Wang, Rodrigo A. Gallardo, Susan J. Lamont, Adrian Smith, and Huaijun Zhou. 2018. Immune repertoire analysis during Newcastle Disease Virus infection in two genetically distinct chicken lines. The 14th Congress of the International Society for Developmental and Comparative Immunology, Santa Fe, NX, US.

-K. Chanthavixay,Y. Wang, Saelao, P., T.R. Kelly, J.M. Dekkers, R. Gallardo, S.J. Lamont. Zhou, H. 2018. Transcriptome Analysis in Two Inbred Chicken Lines Showing Sex-Differential

Response to NDV Infection Under Heat Stress. Plant & Animal Genome XXVI, San Diego, CA.

-Y. Wang, X. Li, Saelao, P., K. Chanthavixay, T.R. Kelly, J.M. Dekkers, R. Gallardo, S.J. Lamont. Zhou, H. 2018. Transcriptome Analysis of Host Response to NDV Infection under Heat Stress in Two Genetically Distinct Chicken Inbred Lines. Plant & Animal Genome XXVI, San Diego, CA

-Y. Wang, Saelao, P., K. Chanthavixay, T.R. Kelly, J.M. Dekkers, R. Gallardo, S.J. Lamont. Zhou, H. 2018. Genomic Analysis of Responses to Heat Stress in Three Chicken Genetic Lines. Plant & Animal Genome XXVI, San Diego, CA

-Saelao, P., Y. Wang, K. Chanthavixay, D. Bunn, R. Gallardo, T.R. Kelly, S.J. Lamont. Zhou, H.

Proteomic Analysis of Liver Tissue from Heat Stressed and Newcastle Disease Virus

Infected Inbred Chicken Lines.

-S.J. Lamont., C. Schmidt, and Zhou, H. 2018. Role of Animal Genetic Improvement in Enhancing Food Security? Plant & Animal Genome XXVI, San Diego, CA

- A.P. Da Silva, K.A. Schat, R.A. Gallardo. (2018). Understanding Innate Responses to IBV

Using Tracheal Organ Cultures. American Veterinary Medical Association / American

Association of Avian Pathologists (AVMA/AAAP) Annual Meeting, Denver, CO.

-R.A. Gallardo, A.P. Da Silva, H. Zhou, C. Kern. (2018). Tracheal Immune Pathways and its

Virome in Chickens Challenged with Different IBV Genotypes. merican Veterinary Medical

Association / American Association of Avian Pathologists (AVMA/AAAP) Annual Meeting,

Denver, CO.

- Derksen, T., R. Lampron, R.A. Gallardo. (2018). California Backyard Poultry as a Reservoir

for Respiratory Diseases and their Seroprevalence of Salmonella sp. American Veterinary

Medical Association / American Association of Avian Pathologists (AVMA/AAAP) Annual

Meeting, Denver, CO.

- R.A. Gallardo, A.P. Da Silva, S. Egaña, S. Stoute, A. Mete, C, K. Clothier, C. Corsiglia, G.

Cutler, C. Kern, H. Zhou. Coryza Outbreaks in Chickens: Persistence, Molecular and Pathogenic

Characterization. (2018). American Veterinary Medical Association / American Association of

Avian Pathologists (AVMA/AAAP) Annual Meeting, Denver, CO.

-S. Egaña, H. Roh, C. Corsiglia, S. Stoute, H.L. Shivaprasad, B. Crossley, R.A. Gallardo. (2018).

Molecular Surveillance of Avian Reoviruses and Efforts Towards a Comprehensive Classification Method. American Veterinary Medical Association / American Association of Avian Pathologists (AVMA/AAAP) Annual Meeting, Denver, CO.

-S. Egaña, H. Roh, H. Zhou, C. Corsiglia, B. Crossley, R.A. Gallardo. Attempts Towards a

Better Classification of Avian Reovirus Variants. (2018). 67th Western Poultry Disease

Conference (WPDC) Salt Lake City, UT.

-A. Figueroa, D. Rejmanek, T. Derksen, R. Lampron, B. Crossley, R.A. Gallardo. (2018).

Persistence of LPAI and HPAI in Treated and Reused Poultry Litter. 67th Western Poultry

Disease Conference (WPDC) Salt Lake City, UT.

-R.A. Gallardo, C. Corsiglia, S. Stoute, A. Mete, A.P. Da Silva, K. Clothier, C. Kern, H. Zhou.

(2018). Understanding Coryza Outbreaks, Persistence and Molecular Biology. 67th Western

Poultry Disease Conference (WPDC) Salt Lake City, UT.

-Derksen, T., R. Lampron, R.A. Gallardo. (2018). Backyard Poultry Flocks Salmonella sp.

Seroprevalence. 67th Western Poultry Disease Conference (WPDC) Salt Lake City, UT.

-Da Silva, A.P., R. Hauck, H. Zhou, R.A. Gallardo. (2018). Understanding Immune Resistance

to Different Infectious Bronchitis Virus Genotypes. 67th Western Poultry Disease Conference

(WPDC) Salt Lake City, UT.

-Li, J., Z. H. Helal, C. Karch, P. Burkhard and M.I. Khan. The Development of a Vaccine

Prototype for Infectious Bronchitis Virus Using Peptide Nanoparticles AVMA, Convention,

Indianapolis, IN, July 22-25, 2017.

-Khan, M. I, Li, J., Z. H. Helal, C. Karch, P. Burkhard. Peptide Nanoparticle-based Vaccine

Induced Immune Responses against Infection of High Pathogenicity Avian Influenza Virus

in Chickens. AVMA, Convention, Indianapolis, IN, July 22-25, 2017.

-Ortega-Heinly Nastassja, T. Girshick, M. I. Khan. Expression of Chicken Parvovirus

Page 3 Structural Proteins VP1 and VP2 Using a Pichia pastoris System. AVMA, Convention,

Indianapolis, IN, July 22-25, 2017.

-Lopardo Roxanne, N. Ortega-Heinly, T. Girshick and M. I. Khan. Enzyme-Linked

Immunosorbent Assay Using Chicken Parvovirus Recombinant VP1 and VP2 derived from a

Pichia pastoris Expression System. AVMA, Convention, Indianapolis, IN, July 22-25, 2017.

-Khan, M. I. Molecular diagnostic tests and novel vaccines for poultry diseases. Beijing

Academy of Agriculture Seminar series. Beijing China, August 18, 2017.

-Khan, M. I. & P. Burkhard. Development of novel nanoparticle-based vaccines for infectious

bronchitis virus in chickens. PRD-CAP, 3rd Annual PRD-CAP meeting, San Diego,

California. October 8, 2017.

-Khan M. I. Novel nanoparticle based IBV vaccine. Shandong Veterinary Research Institute,

Jinan city, Shandong, China. August 23, 2018.

-In vitro replication of Marek’s disease virus (MDV) does not require infected cell protein 27

(ICP27). 43rd Annual International Herpesvirus Workshop. University of British Columbia,

Vancouver, Canada, July 21-25, 2018.

-Regulation of Marek’s disease virus (MDV) glycoprotein C (gC) by infected cell protein 27

(ICP27). 43rd Annual International Herpesvirus Workshop. University of British Columbia,

Vancouver, Canada, July 21-25, 2018.

-Determining the role Marek’s disease virus (MDV) conserved herpesvirus protein kinase

(CHPK) plays in host-to-host transmission. 43rd Annual International Herpesvirus Workshop.

University of British Columbia, Vancouver, Canada, July 21-25, 2018.

-Reynolds, Donald L. Oral presentation. Developing and Evaluating a Biosecurity Plan.

Nebraska Poultry Industries Annual Meeting. February 21-22, 2018. Columbus, NE.

-Reynolds, Donald L. Wayne Woldt and Marcia Oetjen. The Big Red Biosecurity Program.

Oral presentation with abstract. 69th North Central Avian Disease Conference, Minneapolis,

March 12-13, 2018.

-Reynolds, Donald L. Wayne Woldt and Marcia Oetjen. The Big Red Biosecurity Program

for Poultry Develops Drone Technology for Biosurveillance. Poster presentation.

American Association of Avian Pathologists / American Veterinary Medical Association

annual convention meeting. Denver, CO. July 13 – 17, 2018.

-Woldt, Wayne E., Ph.D.,P.E., Reynolds, Donald L. DVM, Ph.D., Oetjen, M., and Bhatti, S.

Unmanned Aircraft Systems for Enhanced Biosecurity Through Aerial Surveillance. Oral

paper presentation. Session title: Biosecurity for Animal Facilities. Annual International

Meeting of the American Society of Agricultural and Biological Engineers, Detroit, MI –

July 29-Aug 3, 2018.

-Habanineza, Algarde Jefferson and Donald L. Reynolds. Big Red Biosecurity Program.

Poster presentation. To be presented at the College of Agriculture and Natural Resource’s

UNL/Reynolds 5 Undergraduate Scholars Program. August 17, 2018.

-Ngunjiri JM, Abundo MC, Jang H, Elaish M, KC M, Ghorbani A, Youmans BP, Johnson TJ, Lee

Understanding the respiratory microbiome of commercial turkeys and chicken layers. 98th

Annual Conference of Research Workers in Animal Diseases meeting. Chicago, IL. December

3-5, 2017.

-Jang H, Ngunjiri JM, Elaish M, Lee CW. Efficacy and Synergy of Live-attenuated and

Inactivated Influenza Vaccines in Young Chickens. 98th Annual Conference of Research

Workers in Animal Diseases meeting. Chicago, IL. December 3-5, 2017.

-Michael C. Abundo, John M. Ngunjiri, Hyesun Jang, Mohamed Elaish, Mahesh KC, Amir

Ghorbani, Bonnie P. Youmans, Timothy J. Johnson, Chang-Won Lee. Successional changes in

respiratory microbiome in clinically healthy chicken layers. 98th Annual Conference of Research

Workers in Animal Diseases meeting. Chicago, IL. December 3-5, 2017.

-Abundo MC, Ngunjiri JM, Jang H, Elaish M, KC M, Ghorbani A, Youmans BP, Johnson TJ, Lee

Successional changes in respiratory microbiome and variation of microbiome in the upper

and lower respiratory tract in clinically healthy chicken layers. 69th North Central Avian Disease

Conference. Minneapolis, MN. March 12-13, 2018.

-Ngunjiri JM, Elaish M, Abundo MC, Ghorbani A, Jang H, KC M, Lee CW. Influenza virus

infection and respiratory microbiome of turkeys. 10th International Symposium on Avian

Influenza. Brighton, UK. April 15-18, 2018.

-Ngunjiri JM, KC M, Abundo MC, Elaish M, Ghorbani A, Jang H, Lee CW. Influenza virus

infection and respiratory microbiome in chickens infected with infectious bursal disease virus.

10th International Symposium on Avian Influenza. Brighton, UK. April 15-18, 2018.

-Lee CW, Jang H, Ngunjiri JM, Elaish M, Ghorbani A, KC M, Abundo M. Prime-boost vaccination

using live and inactivated virus vaccines for broader protection against avian influenza. 10th

International Symposium on Avian Influenza. Brighton, UK. April 15-18, 2018.

-Abundo MC, Ngunjiri JM, Jang H, Elaish M, KC M, Ghorbani A, Youmans BP, Jonson TJ, Lee

Microbiome in the upper and lower respiratory tract and standardization of sample

collection methods in clinically healthy chicken layers. AVMA/AAAP Annual Convention,

Denver, CO. July 13-17, 2018.

-Maekawa, D., G. Beltrán, S. M. Riblet and M. García. Transmission assessment of infectious

laryngotracheitis virus (ILTV) in recombinant (r)HVT-ILT in ovo vaccinated broilers after

experimental challenge. Annual meeting of the American Association of Avian Pathologists

(AAAP). Oral presentation. Denver, CO., July 13 to 14, 2018.

-Beltrán, G., S. M. Riblet, D. J. Hurley, M. García. Changes in CD4+/CD8+ T cells in the

conjunctiva associated-lymphoid tissue (CALT) and Harderian gland (HG) during infection or

vaccination with Infectious laryngotracheitis virus (ILTV). Annual meeting of the American

Association of Avian Pathologists (AAAP). Poster presentation. Denver, CO., July 13 to 14, 2018.

-Loncoman, C. A., M. García, and S. M. Riblet and J. M. Devlin. Assessment of infectious

laryngotracheitis virus (ILTV) recombination in non-vaccinated and vaccinated chickens after coinfection with genotype V and VI virulent strains of the virus. Annual meeting of the American Association of Avian Pathologists (AAAP). Oral presentation. Denver, CO., July 13 to 14, 2018.

-García, M. Infectious laryngotracheitis commercial recombinant vaccines and vaccination

strategies. Annual meeting of the American Association of Avian Pathologists (AAAP). Oral

presentation. Denver, CO., July 13 to 14, 2018.

-S. J. Spatz, García, M., W. Fuchs, T. Ross, S. M. Riblet, T. Kim, C. A. Loncoman and J. Volkening. Reconstitution of the Infectious Laryngotracheitis Virus using Bacterial and Yeast Genomic Assembly. Annual meeting of the American Association of Avian Pathologists (AAAP). Oral presentation. Denver, CO., July 13 to 14, 2018.

-García, M. Infectious laryngotracheitis vaccination strategies. Poultry Health Day. Stratford,

Ontario Canada. June 20, 2018.

-Maekawa, D., G. Beltrán, S. M. Riblet and M. García. Protection efficacy of a Herpesvirus of

Turkey (HVT) recombinant vaccine against Infectious laryngotracheitis virus (ILTV) in broilers

administered in ovo at three standardized doses. 2nd International Avian Respiratory Disease

Conference. Oral presentation. Athens, GA., May 29 to June 1, 2018

-Beltrán, G., S. M. Williams, G. Zavala, J. S. Guy and M. García. The replication patterns of

infectious laryngotracheitis virus (ILTV) pathogenic strain and chicken embryo origin (CEO)

vaccine are ruled by the route of inoculation. 2nd International Avian Respiratory Disease

Conference. Oral presentation. Athens, GA., May 29 to June 1, 2018

-García, M. Vaccination against infectious laryngotracheitis (ILT). What we have learned and the

knowledge gaps that remain. 2nd International Avian Respiratory Disease Conference. Oral

presentation. Athens, GA., May 29 to June 1, 2018.

-Maekawa, D., G. Beltrán, S. M. Riblet and M. García. Protection efficacy of a Herpesvirus of

Turkey (HVT) recombinant vaccine against Infectious laryngotracheitis virus (ILTV) in broilers

administered in ovo at three standardized doses. Southern Conference for Avian Diseases (SCAD) Oral presentation, Atlanta, GA, January 29-30, 2018.

-Investigation of Environmental Fomites and Their Role in the Transmission of Mycoplasma

synoviae. Brandon T. Armwood, Abigail Reith, Luke Baldwin, Nils Schoof, Rosetta Barber,

Rachel Jude, Marianne Dos Santos and Naola Ferguson-Noel). American Veterinary Medical

Association (AVMA) Annual Convention, Denver, CO. July 13th -17th, 2018.

-Development of Mycoplasma gallisepticum F- Strain Vaccine Specific PCR Protocols. Naola

Ferguson-Noel, Marianne Dos Santos, and John Maurer. American Veterinary Medical

Association (AVMA) Annual Convention, Denver, CO. July 13th -17th, 2018.

-The Development of Real time PCR protocols to Differentiate Mycoplasma synoviae Vaccine

and Field Strains. Mohammadreza Ehsan, Marianne Dos Santos, Amanda Olivier and Naola

Ferguson-Noel. American Veterinary Medical Association (AVMA) Annual Convention,

Denver, CO. July 13th -17th, 2018.

-Tracheal Lesion Evaluation of broilers co-infected with Mycoplasma synoviae and Infectious

Laryngotracheitis. Valerie C Marcano, Susan M Williams, Maricarmen García, Marianne Dos

Santos, and Naola Ferguson-Noel. American Veterinary Medical Association (AVMA) Annual

Convention, Denver, CO. July 13th -17th, 2018.

-Aston, E.J., B. J. Jordan, M. Garcia and M.W. Jackwood. Effect of pullet Vaccination on

Development and Longevity of Immunity. American Association of Avian Pathologists,

AVMA Convention. Indianapolis, IN, July 2017

Reported Grants:

-Toro H., Q. Yu, V.L. van Santen, F.W. van Ginkel, K. Joiner. Infectious Bronchitis Virus S2

Expressed from Recombinant Virus to Confer Protection across Serotypes in Chickens. USDA/AFRI Ohio State University, 2015-2020. $246,158 (Collaboration with SEPRL)

-Toro, H., V.L. van Santen, R. Cattley, K. Joiner. Early Vaccination of Chickens Significantly

Decreases Induction of Infectious Bronchitis Virus Specific Immunity. USDA NIFA #2016-

67015-24916 2016-2019, $498,988

-The project titled “Improving food security in Africa by enhancing resistance to disease and heat in chickens; Feed the future innovation lab for genomics to improve poultry”, USAID AID-OAA-A- 13-00080 H. Zhou, T. Kelly, R. Gallardo, S. J. Lamont, J. Dekkers etc.

-Jarosinski, K.W. (PD) and Grose, C. (Co-PD). The role of the conserved alphaherpesvirus

glycoprotein C in host-to-host transmission. NIH/USDA-NIFA-AFRI #2018-08536; 2019-

2023, $1,624,996.

-Jarosinski, K.W. (PD). Determining the role of Marek’s disease virus UL13 protein kinase in

horizontal transmission. USDA-NIFA-AFRI #2016-67015-26777; 2016-2019, $499,838.

-Jarosinski, K.W. (PD. Determining viral factors important for generation of cell-free Marek’s

disease vaccines. USDA-NIFA-AFRI #2013-67015-26787; 2013-2016, $499,807.

-Reynolds, D. L. and W. Woldt. USDA contract for the Poultry Respiratory Disease Coordinated

Agricultural Program. $25,000/ year for 2 years. Big Red Biosecurity Program: A Comprehensive

Educational Biosecurity Program for Nebraska Poultry That Explores Use of Unmanned Aircraft

Systems (Drone) Technology. 2018. 2nd year funded Sept. 2018.

-Reynolds, D. L. and Jay Reddy. USDA contract for the Poultry Respiratory Disease Coordinated

Agricultural Program. $25,000. Immune Conditioning for Better Respiratory Disease Protection.

Funded July, 2018.

-Reynolds, D. L. Nebraska Poultry Industries. Biosecurity Assessment of In-House Composting for

Use with Routine Mortalities. $5,000. Sept. 2018.

-Lee CW. USDA contract (SCA58-6040-7-005). $65,000. Transmission of influenza viruses

to turkeys. 2018.

12/26/2018